776 research outputs found

    Distribution and toxicity of Cylindrospermopsis raciborskii (Cyanobacteria) in Portuguese freshwaters

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    The cyanobacterium Cylindrospermopsis racborskii has become increasingly prevalent in freshwaters worldwide. This species is of concern from a water quality perspective due to its known ability to produce a potent hepatotoxic alkaloid cylindrospermopsin, which has been implicated in outbreaks of human sickness and cattle mortality. C. raciborskii isolates from Brazil have also been found to produce the highly toxic paralytic shellfish poisons (PSP's). In this paper we report the toxicity of four isolates of C. raciborskii taken from three reservoirs and one river in Portugal as well as the occurrence of this species in other water bodies used for potable and recreational purposes. All four isolates grown in pure culture in the laboratory were found to be toxic in the mouse bioassay at 8 - 24 hours after intraperitoneal administration of single doses ranging from 1337 to 1572 mg kg-1. Histological examination showed liver damage as the primary lesion, in addition to some inflammation in the intestine. HPLC/MS tests for the presence of cylindrospermopsin, microcystins and PSP toxins were negative. The available evidence suggests that another toxin may be present. This report constitutes the first report of toxic C. raciborskii in Europe and draws attention to our need for increased monitoring of this cyanobacterium in water bodies used for potable and recreational purposes.La cianobacteria Cylindrospermopsis racborskii ha incrementado su presencia en agua dulce por todo el mundo. Esta especie es de gran importancia debido a su conocida capacidad para producir un alcaloide hepatotóxico, cilindrospermopsina. Esta toxina es responsable de enfermedades en humanos y mortalidad en ganado. Cepas de C. raciborskii aisladas de Brasil se ha demostrado que tienen capacidad para producir la toxina paralytic shellfish poisons (PSP's). En este trabajo hemos estudiado la presencia de esta especie en agua dulce con usos recreacionales y de abastecimiento en Portugal. De las cuatro cepas C. raciborskii aisladas de tres embalses y de un río estudiamos la toxicidad con bioensayos en ratones. Todas presentaron toxicidad al cabo de las 8 - 24 horas tras inyección intraperitoneal, la dosis presenta un rango de concentración de 1337 a 1572 mg kg-1. La examinación histológica reveló daños en el hígado y señales inflamatorias en el intestino. Los análisis con HPLC/MS revelaron la ausencia de cilindrospermopsina, microcistinas y PSP, sugiriendo que otra toxina podría estar presente para las cepas aisladas y cultivadas en laboratorio. Este artículo es el primero acerca de la toxicidad de C. raciborskii en Europa y refleja la necesidad de aumentar el monitoreo de esta cyanobacteria en el agua potable y con fines recreativos

    Relaxation bottleneck and its suppression in semiconductor microcavities

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    A polariton relaxation bottleneck is observed in angle-resolved measurements of photoluminescence emission from a semiconductor microcavity. For low power laser excitation, low k polariton states are found to have a very small population relative to those at high k. The bottleneck is found to be strongly suppressed at higher powers in the regime of superlinear emission of the lower polariton states. Evidence for the important role of carrier-carrier scattering in suppression of the bottleneck is presented

    Market segmentation strategies for complex automotive products

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    With the advent of 'big data', the purpose of this empirical study was to take the opportunity to rethink conventional market segmentation strategies. This is particularly relevant for the automotive industry which is going through a period of rapid change with advanced technologies such as electric powered and autonomous vehicles, creating increased concerns as to how this complexity is communicated effectively. A mixed methods approach was utilised to collect data from multiple sources, incorporating in-depth discussion groups, semi-structured interviews, an online survey, and data collection of communication processes through the attendance of new car product launches. The results suggest that marketing departments should rethink their data capture methods to collect more relevant consumer information, not the contemporary trend of needs, attitude, and motivation variables that are difficult to identify and collect, but basic information on their level of familiarity with products through previous experience and exposure. The basic dimensions identified are characterised by a consumer's expertise, involvement, and familiarity with a product. The findings are synthesised into a theoretical framework to define differing levels of product complexity, which would enable manufacturers to provide more closely defined market segmentation strategies when communicating new product information

    Exploring the Impact of Different Durations of Foam Rolling as a Recovery Technique following Intense Exercise in College-aged Males

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    Background: Foam rolling (FR) for recovery from delayed onset muscle soreness (DOMS) and exercise induced muscle damage (EIMD) has received considerable attention because the technique is being a relatively inexpensive and is self-performed. However, there are currently no FR guidelines and the available literature assessing the impact of FR for recovery of muscle soreness is heterogeneous and offers conflicting results. Assessing different FR durations and their impact on recovery may help provide insight to the effectiveness of this technique for recovery from EIMD and DOMS. Purpose: To explore the impact of two different acute durations of FR for the recovery of vertical jump (VJ), sprint speed (SS), agility, range of motion (ROM), and pain/soreness following high intensity exercise. Methods: An experimental, randomized cross-over design was used consisting of twelve college-aged males were randomly assigned to a one- or two-minute FR group (EXP) (N = 6 per group) and served as their own control (CON). Participants completed a familiarization and baseline measure session before completing two, 4-session testing weeks (1-week EXP, 1-week CON) separated by a 1-week washout period. Session one employed the EIMD protocol and immediate post exercise measures taken. Sessions 2-4 were 24, 48, and 72 hr. post measures. FR (either 1 or 2-minutes) was completed during the EXP week on each lower extremity muscle group following the immediate post exercise measures, whereas CON did not complete FR. Results: No significant difference was seen between EXP groups at any time point post exercise for recovery of jump height (F=.007, P=.933), agility (F=.171, P=.681), sprint speed (F=.024, P=.876), ROM (F=.013, P=1.000), or pain/soreness (F=.000, P=.909). Conclusion: Foam rolling for either 1 or 2-minutes per muscle group immediately post exercise did not significantly aid in recovery of muscle soreness as measured by its impact on performance and non-performance outcomes. Therefore, FR using the protocol applied in this study, in the lower extremities immediately following high-intensity exercise may not be beneficial for recovering from DOMS in college-aged males
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