Early human lung immune cell development and its role in epithelial cell fate

Studies of human lung development have focused on epithelial and mesenchymal cell types and function, but much less is known about the developing lung immune cells, even though the airways are a major site of mucosal immunity after birth. An unanswered question is whether tissue-resident immune cells play a role in shaping the tissue as it develops in utero. Here, we profiled human embryonic and fetal lung immune cells using scRNA-seq, smFISH, and immunohistochemistry. At the embryonic stage, we observed an early wave of innate immune cells, including innate lymphoid cells, natural killer cells, myeloid cells, and lineage progenitors. By the canalicular stage, we detected naive T lymphocytes expressing high levels of cytotoxicity genes and the presence of mature B lymphocytes, including B-1 cells. Our analysis suggests that fetal lungs provide a niche for full B cell maturation. Given the presence and diversity of immune cells during development, we also investigated their possible effect on epithelial maturation. We found that IL-1β drives epithelial progenitor exit from self-renewal and differentiation to basal cells in vitro. In vivo, IL-1β-producing myeloid cells were found throughout the lung and adjacent to epithelial tips, suggesting that immune cells may direct human lung epithelial development

Direction Reconstruction using a CNN for GeV-Scale Neutrinos in IceCube

The IceCube Neutrino Observatory observes neutrinos interacting deep within the South Pole ice. It consists of 5,160 digital optical modules, which are embedded within a cubic kilometer of ice, over depths of 1,450 m to 2,450 m. At the lower center of the array is the DeepCore subdetector. Its denser sensor configuration lowers the observable energy threshold to the GeV-scale, facilitating the study of atmospheric neutrino oscillations. The precise reconstruction of neutrino direction is critical in the measurements of oscillation parameters. This work presents a method to reconstruct the zenith angle of GeV-scale events in IceCube by using a convolutional neural network and compares the result to that of the current likelihood-based reconstruction algorithm

A Search for Neutrino Sources with Cascade Events in IceCube

IceCube has discovered a flux of astrophysical neutrinos, and more recently has used muon-neutrino datasets to present evidence for one source; a flaring blazar known as TXS 0506+056. However, the sources responsible for the majority of the astrophysical neutrino flux remain elusive. Opening up new channels for detection can improve sensitivity and increase the discovery potential. In this work we present a new neutrino dataset relying heavily on Deep-Neural-Networks (DNN) to select cascade events produced from neutral-current interactions of all flavors and charged-current interactions with flavors other than muon-neutrino. The speed of DNN processing makes it possible to select events in near realtime with a single GPU. Cascade events have reduced angular resolution when compared to muon-neutrino events, however the resulting dataset has a lower energy threshold in the Southern Sky and a lower background rate. These benefits lead to an factor of 2-3 improvement in sensitivity to sources in the Southern Sky when compared to muon-neutrino datasets. This dataset is particularly promising for identifying transient neutrino sources in the Southern Sky and neutrino production from the galactic plane

Performance studies for a next-generation optical sensor for IceCube-Gen2

We present performance studies of a segmented optical module for the IceCube-Gen2 detector. Based on the experience gained in sensor development for the IceCube Upgrade, the new sensor will consist of up to eighteen 4 inch PMTs housed in a transparent pressure vessel, providing homogeneous angular coverage. The use of custom molded optical `gel pads\u27 around the PMTs enhances the photon capture rate via total internal reflection at the gel-air interface. This contribution presents simulation studies of various sensor, PMT, and gel pad geometries aimed at optimizing the sensitivity of the optical module in the face of confined space and harsh environmental conditions at the South Pole

Three-year performance of the IceAct telescopes at the IceCube Neutrino Observatory

IceAct is an array of compact Imaging Air Cherenkov Telescopes at the ice surface as part of the IceCube Neutrino Observatory. The telescopes, featuring a camera of 61 silicon photomultipliers and fresnel-lens-based optics, are optimized to be operated in harsh environmental conditions, such as at the South Pole. Since 2019, the first two telescopes have been operating in a stereoscopic configuration in the center of IceCube\u27s surface detector IceTop. With an energy threshold of about 10 TeV and a wide field-of-view, the IceAct telescopes show promising capabilities of improving current cosmic-ray composition studies: measuring the Cherenkov light emissions in the atmosphere adds new information about the shower development not accessible with the current detectors. First simulations indicate that the added information of a single telescope leads, e.g., to an improved discrimination between flux contributions from different primary particle species in the sensitive energy range. We review the performance and detector operations of the telescopes during the past 3 years (2020-2022) and give an outlook on the future of IceAct

Highlights from the IceCube Neutrino Observatory

As IceCube surpasses a decade of operation in the full detector configuration, results that drive forward the fields of neutrino astronomy, cosmic ray physics, multi-messenger astronomy, particle physics, and beyond continue to emerge at an accelerated pace. IceCube data is dominated by background events, and thus teasing out the signal is the common challenge to most analyses. Statistical accumulation of data, along with better understanding of the background fluxes, the detector, and continued development of our analysis tools have produced many profound results that were presented at ICRC2023. Highlights covered here include the first neutrino observation of the Galactic Plane, the first observation of a steady emission neutrino point source NGC1068, new characterizations of the cosmic ray flux and its secondary particles, and a possible new era in measuring the energy spectrum of the diffuse astrophysical flux. IceCube is poised to make more discoveries and drive fields forward in the near future with many novel analyses coming online