Simultaneous and Sensitive Analysis of THC, 11-OH-THC, THC-COOH, CBD, and CBN by GC-MS in Plasma after Oral Application of Small Doses of THC and Cannabis Extract

Besides the psychoactive Δ9-tetrahydrocannabinol (THC), hashish and marijuana as well as cannabis-based medicine extracts contain varying amounts of cannabidiol (CBD) and of the degradation product cannabinol (CBN). The additional determination of these compounds is interesting from forensic and medical points of view because it can be used for further proof of cannabis exposure and because CBD is known to modify the effects of THC. Therefore, a method for the simultaneous quantitative determination of THC, its metabolites 11-hydroxy-Δ9-tetrahydrocannabinol (11-OH-THC) and 11-nor-9-carboxy-Δ9-tetrahydrocannabinol (THC-COOH), CBD and CBN from plasma was developed. The method was based on automatic solid-phase extraction with C18 ec columns, derivatization with N,O-bistrimethylsilyltrifluoroacetamide (BSTFA), and gas chromatography-electron impact ionization-mass spectrometry (GC-EI-MS) with deuterated standards. The limits of detection were between 0.15 and 0.29 ng/mL for THC, 11-OH-THC, THC-COOH, and CBD and 1.1 ng/mL for CBN. The method was applied in a prospective pharmacokinetic study after single oral administration of 10 mg THC alone or together with 5.4 mg CBD in cannabis extract. The maximum plasma concentrations after cannabis extract administration ranged between 1.2 and 10.3 ng/mL (mean 4.05 ng/mL) for THC, 1.8 and 12.3 ng/mL (mean 4.9 ng/mL) for 11-OH-THC, 19 and 71 ng/mL (mean 35 ng/mL) for THC-COOH, and 0.2 and 2.6 ng/mL (mean 0.95 ng/mg) for CBD. The peak concentrations (mean values) of THC, 11-OH-THC, THC-COOH, and CBD were observed at 56, 82, 115, and 60 min, respectively, after intake. CBN was not detected. Caused by the strong first-pass metabolism, the concentrations of the metabolites were increased during the first hours after drug administration when compared to literature data for smoking. Therefore, the concentration ratio 11-OH-THC/THC was discussed as a criterion for distinguishing oral from inhalative cannabis consumptio

PV-0327 Patient-specific motion management and adaptive respiratory gating in Pancreatic SBRT

Digitalitzat per Artypla

Discretisation of the Maxwell equations on tetrahedral grids

The aim of this report is to describe the discretisation of the Maxwell equations on tetrahedral grids with corresponding dual Voronoi cells to explain the resulting program. The symmetry of the coefficients of the matrix is proven. A small example shows an input file and same other details

Measuring the behavior of the acoustic standing wave exiting a flue organ pipe

Correctly predicting the playing frequencies of a musical instrument is dependent on the length of the resonator with the addition of an end correction. There are multiple theories describing this end correction, perhaps the simplest being that the end correction of a pipe is a physical extension of the sinusoidal pressure standing wave inside the pipe. However, recent optical imaging of the flow in a flue organ pipe found an unexpected exponential decay of pressure just outside of the pipe. This work looks to validate those findings acoustically. A flue organ pipe was played at the 1st, 5th, and 7th harmonics and the pressure just inside and immediately outside the end of the pipe played was measured using a zero-degree PU Match Microflown sound intensity probe. These measurements were fit to both exponential and sinusoidal curves and compared to the optical images. While an exponential trend is in fact apparent in some cases, the goodness-of-fit appears to be dependent on which harmonic is sounding. Future work includes exploration of a potential transitional region, assessing the impact of altered pipe geometry (both cross-sectional shape and size), and investigating potential sensor interference by using other measurement equipment

Simulation of microwave and semiconductor laser structures including PML: Computation of the eigen mode problem, the boundary value problem, and the scattering matrix

The properties of microwave circuits and optical structures can be described in terms of their scattering matrix which is extracted from the orthogonal decomposition of the electric field. We discretize the Maxwell' equations with ortogonal grids using the Finite Integration Technique (FIT). Maxwellian grid equations are formulated for staggered nonequidistant rectangular grids and for tetrahedral nets with corresponding dual Voronoi cells. The surface of the computation domain is assumed to be an electric or magnetic wall, open-region problems require uniaxial Perfectly Matched Layer (PML) absorbing boundary conditions. Calculating the excitations at the ports, one obtains eigenvalue problems and then large-scale systems of linear algebraic equations. This paper is a revised version of the preprint no. 987