Balancing Selection Pressures, Multiple Objectives, and Neural Modularity to Coevolve Cooperative Agent Behavior

Previous research using evolutionary computation in Multi-Agent Systems indicates that assigning fitness based on team vs.\ individual behavior has a strong impact on the ability of evolved teams of artificial agents to exhibit teamwork in challenging tasks. However, such research only made use of single-objective evolution. In contrast, when a multiobjective evolutionary algorithm is used, populations can be subject to individual-level objectives, team-level objectives, or combinations of the two. This paper explores the performance of cooperatively coevolved teams of agents controlled by artificial neural networks subject to these types of objectives. Specifically, predator agents are evolved to capture scripted prey agents in a torus-shaped grid world. Because of the tension between individual and team behaviors, multiple modes of behavior can be useful, and thus the effect of modular neural networks is also explored. Results demonstrate that fitness rewarding individual behavior is superior to fitness rewarding team behavior, despite being applied to a cooperative task. However, the use of networks with multiple modules allows predators to discover intelligent behavior, regardless of which type of objectives are used

Impact of marine mercury cycling on coastal atmospheric mercury concentrations in the North- and Baltic Sea region

The cycling of mercury between ocean and atmosphere is an important part of the global Hg cycle. Here we study the regional contribution of the air-sea exchange in the North- and Baltic Sea region. We use a newly developed coupled regional chemistry transport modeling (CTM) system to determine the flux between atmosphere and ocean based on the meteorological model COSMO-CLM, the ocean-ecosystem model ECOSMO, the atmospheric CTM CMAQ and a newly developed module for mercury partitioning and speciation in the ocean (MECOSMO). The model was evaluated using atmospheric observations of gaseous elemental mercury (GEM), surface concentrations of dissolved gaseous mercury (DGM), and air-sea flux (ASF) calculations based on observations made on seven cruises in the western and central Baltic Sea and three cruises in the North Sea performed between 1991 and 2006. It was shown that the model is in good agreement with observations: DGM (Normalized Mean Bias NMB=-0.27 N=413), ASF (NMB=-0.32, N=413), GEM (NMB=0.07, N=2359). Generally, the model was able to reproduce the seasonal DGM cycle with the best agreement during winter and autumn (NMBWinter=-0.26, NMBSpring=-0.41, NMBSummer=-0.29, NMBAutumn=-0.03). The modelled mercury evasion from the Baltic Sea ranged from 3400 to 4000 kg/a for the simulation period 1994–2007 which is on the lower end of previous estimates. Modelled atmospheric deposition, river inflow and air-sea exchange lead to an annual net Hg accumulation in the Baltic Sea of 500 to 1000 kg/a. For the North Sea the model calculates an annual mercury flux into the atmosphere between 5700 and 6000 kg/a. The mercury flux from the ocean influenced coastal atmospheric mercury concentrations. Running CMAQ coupled with the ocean model lead to better agreement with GEM observations. Directly at the coast GEM concentrations could be increased by up to 10% on annual average and observed peaks could be reproduced much better. At stations 100km downwind the impact was still observable but reduced to 1–3%

On-chip high-speed sorting of micron-sized particles for high-throughput analysis

A new design of particle sorting chip is presented. The device employs a dielectrophoretic gate that deflects particles into one of two microfluidic channels at high speed. The device operates by focussing particles into the central streamline of the main flow channel using dielectrophoretic focussing. At the sorting junction (T- or Y-junction) two sets of electrodes produce a small dielectrophoretic force that pushes the particle into one or other of the outlet channels, where they are carried under the pressure-driven fluid flow to the outlet. For a 40mm wide and high channel, it is shown that 6micron diameter particles can be deflected at a rate of 300particles/s. The principle of a fully automated sorting device is demonstrated by separating fluorescent from non-fluorescent latex beads

Development and validation of standardized sampling protocols for assessing freshwater mussel populations in Missouri

Freshwater mussels are among the most threatened aquatic fauna in North America. Given the need for continued monitoring of threatened mussel species, an evaluation of the effectiveness of metrics from visually-based sampling methods is an essential step in assessing populations. We surveyed 14 sites in the Meramec River Basin in East-central Missouri to determine the effectiveness of visual sampling methods and to determine the effects of habitat variability on the effectiveness of these methods. At each site, we employed three visual methods for estimating mussel abundance; timed visual searches (TVS), systematic strip transects, and stratified randomly placed visual 0.25 m2 quadrats. We excavated substrate at each 0.25 m2 quadrat to determine baseline densities for each species. We compared the results of density estimates or CPUE of each visual method with density estimates based on excavated quadrat samples, which we considered the most rigorous of our sampling approaches and a useful baseline. Sampling efficiencies (SE) of visual-based quadrats were calculated and used to determine if visual techniques provide reasonable approximations of overall mussel abundances and to investigate factors that influenced SE. Sampling efficiency averaged 0.45 (Std. Err. = 0.05) across species, and varied greatly among taxa and the 3 rivers included in this study, and was influenced by temperature, discharge, and, to a lesser extent, by other factors. Correspondence of TVS and transects with excavation varied amongst taxa, but was typically low, and was affected inconsistently by habitat factors. Species detection probabilities were highest for TVS (0.45 - 1.00, mean = 0.84) and lowest for visual quadrats (0.17 - 1.00, mean = 0.71; strip transects had intermediate values (0.23 - 1.00, mean = 0.75). Detection probabilities for most species were not affected by habitat variability, those that were affected, were inconsistent. These results will be used to produce validated and standardized sampling protocols for freshwater mussels of Missouri

Long-term retrospective analysis of mackerel spawning in the North Sea: a new time series and modeling approach to CPR data

We present a unique view of mackerel (Scomber scombrus) in the North Sea based on a new time series of larvae caught by the Continuous Plankton Recorder (CPR) survey from 1948-2005, covering the period both before and after the collapse of the North Sea stock. Hydrographic backtrack modelling suggested that the effect of advection is very limited between spawning and larvae capture in the CPR survey. Using a statistical technique not previously applied to CPR data, we then generated a larval index that accounts for both catchability as well as spatial and temporal autocorrelation. The resulting time series documents the significant decrease of spawning from before 1970 to recent depleted levels. Spatial distributions of the larvae, and thus the spawning area, showed a shift from early to recent decades, suggesting that the central North Sea is no longer as important as the areas further west and south. These results provide a consistent and unique perspective on the dynamics of mackerel in this region and can potentially resolve many of the unresolved questions about this stock.lved questions about this stoc

Robust self-replication of combinatorial information via crystal growth and scission

Understanding how a simple chemical system can accurately replicate combinatorial information, such as a sequence, is an important question for both the study of life in the universe and for the development of evolutionary molecular design techniques. During biological sequence replication, a nucleic acid polymer serves as a template for the enzyme-catalyzed assembly of a complementary sequence. Enzymes then separate the template and complement before the next round of replication. Attempts to understand how replication could occur more simply, such as without enzymes, have largely focused on developing minimal versions of this replication process. Here we describe how a different mechanism, crystal growth and scission, can accurately replicate chemical sequences without enzymes. Crystal growth propagates a sequence of bits while mechanically-induced scission creates new growth fronts. Together, these processes exponentially increase the number of crystal sequences. In the system we describe, sequences are arrangements of DNA tile monomers within ribbon-shaped crystals. 99.98% of bits are copied correctly and 78% of 4-bit sequences are correct after two generations; roughly 40 sequence copies are made per growth front per generation. In principle, this process is accurate enough for 1,000-fold replication of 4-bit sequences with 50% yield, replication of longer sequences, and Darwinian evolution. We thus demonstrate that neither enzymes nor covalent bond formation are required for robust chemical sequence replication. The form of the replicated information is also compatible with the replication and evolution of a wide class of materials with precise nanoscale geometry such as plasmonic nanostructures or heterogeneous protein assemblies

On the Theory of Evolution Versus the Concept of Evolution: Three Observations

Here we address three misconceptions stated by Rice et al. in their observations of our article Paz-y-Miño and Espinosa (Evo Edu Outreach 2:655–675, 2009), published in this journal. The five authors titled their note “The Theory of Evolution is Not an Explanation for the Origin of Life.” First, we argue that it is fallacious to believe that because the formulation of the theory of evolution, as conceived in the 1800s, did not include an explanation for the origin of life, nor of the universe, the concept of evolution would not allow us to hypothesize the possible beginnings of life and its connections to the cosmos. Not only Stanley Miller’s experiments of 1953 led scientists to envision a continuum from the inorganic world to the origin and diversification of life, but also Darwin’s own writings of 1871. Second, to dismiss the notion of Rice et al. that evolution does not provide explanations concerning the universe or the cosmos, we identify compelling scientific discussions on the topics: Zaikowski et al. (Evo Edu Outreach 1:65–73, 2008), Krauss (Evo Edu Outreach 3:193–197, 2010), Peretó et al. (Orig Life Evol Biosph 39:395–406, 2009) and Follmann and Brownson (Naturwissenschaften 96:1265–1292, 2009). Third, although we acknowledge that the term Darwinism may not be inclusive of all new discoveries in evolution, and also that creationists and Intelligent Designers hijack the term to portray evolution as ideology, we demonstrate that there is no statistical evidence suggesting that the word Darwinism interferes with public acceptance of evolution, nor does the inclusion of the origin of life or the universe within the concept of evolution. We examine the epistemological and empirical distinction between the theory of evolution and the concept of evolution and conclude that, although the distinction is important, it should not compromise scientific logic