U and Th content in the Central Apennines continental crust: a contribution to the determination of the geo-neutrinos flux at LNGS

The regional contribution to the geo-neutrino signal at Gran Sasso National Laboratory (LNGS) was determined based on a detailed geological, geochemical and geophysical study of the region. U and Th abundances of more than 50 samples representative of the main lithotypes belonging to the Mesozoic and Cenozoic sedimentary cover were analyzed. Sedimentary rocks were grouped into four main "Reservoirs" based on similar paleogeographic conditions and mineralogy. Basement rocks do not outcrop in the area. Thus U and Th in the Upper and Lower Crust of Valsugana and Ivrea-Verbano areas were analyzed. Based on geological and geophysical properties, relative abundances of the various reservoirs were calculated and used to obtain the weighted U and Th abundances for each of the three geological layers (Sedimentary Cover, Upper and Lower Crust). Using the available seismic profile as well as the stratigraphic records from a number of exploration wells, a 3D modelling was developed over an area of 2^{\circ}x2^{\circ} down to the Moho depth, for a total volume of about 1.2x10^6 km^3. This model allowed us to determine the volume of the various geological layers and eventually integrate the Th and U contents of the whole crust beneath LNGS. On this base the local contribution to the geo-neutrino flux (S) was calculated and added to the contribution given by the rest of the world, yielding a Refined Reference Model prediction for the geo-neutrino signal in the Borexino detector at LNGS: S(U) = (28.7 \pm 3.9) TNU and S(Th) = (7.5 \pm 1.0) TNU. An excess over the total flux of about 4 TNU was previously obtained by Mantovani et al. (2004) who calculated, based on general worldwide assumptions, a signal of 40.5 TNU. The considerable thickness of the sedimentary rocks, almost predominantly represented by U- and Th- poor carbonatic rocks in the area near LNGS, is responsible for this difference.Comment: 45 pages, 5 figures, 12 tables; accepted for publication in GC

MicroRNA profile before and after antiviral therapy in liver transplant recipients for Hepatitis C virus cirrhosis

BACKGROUND AND AIM: Management of Hepatitis C virus (HCV) recurrence is a major challenge after liver transplantation. Significant dysregulated expression of HCV receptors (i.e. claudin-1, occludin, CD81, Scavenger-receptor Type B1) has been shown recently during HCV infection. This might facilitate hepatocytic entry and reinfection of HCV. MicroRNAs (miRs) play role in the regulation of gene expression. We aimed to characterize miR expression profiles related to HCV infection and antiviral therapy in adult liver transplant recipients, with special emphasis on microRNAs predicted to target HCV receptors. METHODS: Twenty-eight adult liver transplant recipients were enrolled in the study. Paired biopsies were obtained at the time of HCV recurrence and at the end of antiviral treatment. MicroRNAs for HCV receptors were selected using target prediction software. Expression levels of miR-21, miR-23a miR-34a, miR-96, miR-99a*, miR-122, miR-125b, miR-181a-2*, miR-194, miR-195, miR-217, miR-221 and miR-224 were determined by RT-qPCR. RESULTS: miR-99a* and miR-224 expressions were increased in HCV recurrence samples, while miR-21 and miR-194 were decreased in comparison to normal liver tissue. Increased expressions of miR-221, miR-224 and miR-217 were observed in samples taken after antiviral therapy when compared with HCV recurrence samples. High HCV titer at recurrence was associated with higher level of miR-122. CONCLUSIONS: Samples at recurrence of HCV and after antiviral therapy revealed distinct HCV-related microRNA expression profiles, with significant dysregulation of those miRNAs potentially targeting mRNAs of HCV receptors. In particular, miR-194 and miR-21 might be involved in the regulation of HCV receptor proteins' expression during HCV infection and antiviral therapy

Dinaric up-thrusts in the Pliocene evolution of the Central Apennines thrust belt of Italy: the Montagna dei Fiori structure

none5noopenCalamita, Fernando; Pace, Paolo; Scisciani, Vittorio; Properzi, Fabiana; Francioni, MirkoCalamita, Fernando; Pace, Paolo; Scisciani, Vittorio; Properzi, Fabiana; Francioni, Mirk

MicroRNA-224 Targets SMAD Family Member 4 to Promote Cell Proliferation and Negatively Influence Patient Survival

10.1371/journal.pone.0068744PLoS ONE87-POLN

Molecular analysis of pediatric brain tumors identifies microRNAs in pilocytic astrocytomas that target the MAPK and NF-kappa B pathways

RT-qPCR confirms (a) up-regulation of miR-34a, miR-146a, miR-542-3p and miR-503 in pilocytic astrocytomas. (b) low expression of miR-124*, miR-129 and miR-129* in pilocytic astrocytomas. Relative expression shown as Log2 fold change compared to normal adult cerebellum and frontal lobe (normalized to miR-423-3p). Data represent two technical replicates ± SD. (ZIP 516 kb

Reaction of Acyl Chlorides with In Situ Formed Zinc Selenolates: Synthesis of Selenoesters versus Ring-Opening Reaction of Tetrahydrofuran

Attempting to apply thein situproduction of PhSeZnSePh to the synthesis of selenoesters, an unexpected reaction involving the solvent (tetrahydrofuran) was observed and studied. We reported here some evidences about the mechanism and the possibility to control the chemoselectivity of this new reaction that afforded the formation of interesting selenoderivatives in which the selenium moiety and the carboxylic one are spaced by four carbon units

Genome-wide identification of direct HBx genomic targets

Background: The Hepatitis B Virus (HBV) HBx regulatory protein is required for HBV replication and involved in HBV-related carcinogenesis. HBx interacts with chromatin modifying enzymes and transcription factors to modulate histone post-translational modifications and to regulate viral cccDNA transcription and cellular gene expression. Aiming to identify genes and non-coding RNAs (ncRNAs) directly targeted by HBx, we performed a chromatin immunoprecipitation sequencing (ChIP-Seq) to analyse HBV recruitment on host cell chromatin in cells replicating HBV. Results: ChIP-Seq high throughput sequencing of HBx-bound fragments was used to obtain a high-resolution, unbiased, mapping of HBx binding sites across the genome in HBV replicating cells. Protein-coding genes and ncRNAs involved in cell metabolism, chromatin dynamics and cancer were enriched among HBx targets together with genes/ncRNAs known to modulate HBV replication. The direct transcriptional activation of genes/miRNAs that potentiate endocytosis (Ras-related in brain (RAB) GTPase family) and autophagy (autophagy related (ATG) genes, beclin-1, miR-33a) and the transcriptional repression of microRNAs (miR-138, miR-224, miR-576, miR-596) that directly target the HBV pgRNA and would inhibit HBV replication, contribute to HBx-mediated increase of HBV replication. Conclusions: Our ChIP-Seq analysis of HBx genome wide chromatin recruitment defined the repertoire of genes and ncRNAs directly targeted by HBx and led to the identification of new mechanisms by which HBx positively regulates cccDNA transcription and HBV replication

Thick-skinned tectonics and basement-involved fold–thrust belts: insights from selected Cenozoic orogens

International audienceDefining the structural style of fold–thrust belts and understanding the controlling factors are necessary steps towards prediction of their long-term and short-term dynamics, including seismic hazard, and to assess their potential in terms of hydrocarbon exploration. While the thin-skinned structural style has long been a fashionable view for outer parts of orogens worldwide, a wealth of new geological and geophysical studies has pointed out that a description in terms of thick-skinned deformation is, in many cases, more appropriate. This paper aims at providing a review of what we know about basement-involved shortening in foreland fold–thrust belts on the basis of the examination of selected Cenozoic orogens. After describing how structural interpretations of fold–thrust belts have evolved through time, this paper addresses how and the extent to which basement tectonics influence their geometry and their kinematics, and emphasizes the key control exerted by lithosphere rheology, including structural and thermal inheritance, and local/regional boundary conditions on the occurrence of thick-skinned tectonics in the outer parts of orogens

Distinguishing rift-related from inversion-related anticlines: Observations from the Abu Gharadig and Gindi Basins, Western Desert, Egypt

Distinguishing the tectonic origin of anticlinal structures is problematic in regions with a complex history of rifting and inversion. We present the results of seismic mapping, in the form of time-depth (isochron) and time-thickness maps to characterize how sedimentary thickness differentials evolved in response to normal faulting and to inversion events on faults within the Abu Gharadig and Gindi Basins in the Western Desert of Egypt. Late Cretaceous rift-related faults in the Abu Gharadig Basin strike NW-SE, W-E and SW-NE. In the eastern part of the basin, a prominent SW-NE trending interbasinal saddle formed in response to preferential subsidence forming half-grabens to its north-west and southeast, during the Mid-Turonian to Santonian interval. Santonian to Palaeogene inversion in the Abu Gharadig Basin developed on its northern basin margin, the absence of SW-NE striking faults in the eastern central basin resulting in any inversion effects being minor. In the central Gindi Basin, Upper Cenomanian to Lower Turonian SW-NE striking rift faults underwent inversion as early as the Mid-Turonian. The orientation of existing rift faults and modification of the local stress fields control the extent to which inversion was taken up in each basin trough time. The Abu Gharadig and Gindi Basins are two of the rift basins developed in West and Central Africa that underwent rifting, inversion and dextral shearing during the Late Cretaceous. We emphasize the value of high-resolution stratigraphic mapping to characterize short-lived and subtle pop-up events that may have gone unnoticed