The certification of different mass fractions of MON-Ø4Ø32-6 in soya bean powder Certified Reference Materials ERM®-BF410ap, ERM®-BF410bp, ERM®-BF410cp, ERM®-BF410dp and ERM®-BF410ep

This report describes the production of a set of Certified Reference Materials (CRMs), ERM BF410ap, bp, cp, dp and ep, which are certified for their GTS 40-3-2 soya bean (unique identifier MON-Ø4Ø32-6) event mass fractions. These materials were produced following ISO Guide 34:2009 and are certified in accordance with ISO Guide 35:2006. The materials are intended for the calibration or quality control of real-time PCR measurements to identify GTS 40-3-2 soya bean and quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs were accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials panel.JRC.F.6-Reference Material

CERTIFICATION REPORT The certification of different mass fractions of VCO-Ø1981-5 in maize seed powder Certified Reference Materials ERM®-BF438a, ERM®-BF438b, ERM®-BF438c, ERM®-BF438d and ERM®-BF438e

This report describes the production of a set of Certified Reference Materials (CRMs) ERM BF438a, b, c, d and e, which are certified for their VCO-Ø1981-5 mass fractions. These materials were produced following ISO Guide 34:2009 and are certified in accordance with ISO Guide 35:2006. Genetically modified (GM) seeds of the maize event VCO-Ø1981-5 and seeds from a non-GM maize variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM maize powder were prepared gravimetrically. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity with respect to the event VCO-Ø1981-5 of the two powder materials and their water mass fractions. The certified values were confirmed by event-specific real-time PCR as an independent verification method (measurements were within the scope of accreditation to ISO/IEC 17025:2005). The uncertainties of the certified values were estimated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) [4] and include uncertainties relating to possible inhomogeneity (Section 4), instability (Section 5) and characterisation (Section 6). The materials are intended for the calibration or quality control of real-time PCR measurements to identify VCO-Ø1981-5 maize and/or quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs are available in glass bottles containing at least 1 g of dried maize seed powder, which were sealed under atmosphere of argon. The minimum amount of sample of the mixtures to be used and recommend for the blank and pure VCO-Ø1981-5 materials is 200 mg. The CRMs were accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.JRC.D.2-Standards for Innovation and sustainable Developmen

CERTIFICATION REPORT The certification of different mass fractions of MON-Ø4Ø32-6 in soya bean seed powder Certified Reference Materials ERM®-BF410dn and ERM®-BF410gn

This report describes the production of two Certified Reference Materials (CRMs), ERM BF410dn and ERM-BF410gn, which are certified for their GTS 40-3-2 soya bean event mass fractions. These materials were produced following ISO Guide 34:2009 [1] and are certified in accordance with ISO Guide 35:2006 [2]. Genetically modified (GM) seeds of the GTS 40-3-2 soya bean event and seeds from a non-GM soya bean variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM soya bean seed powder were prepared gravimetrically. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity with respect to the GTS 40-3-2 soya bean event of the two powder materials and their water mass fractions. The certified values were confirmed by event-specific real-time PCR as an independent verification method (measurements were within the scope of accreditation to ISO/IEC 17025:2005 [3]). The uncertainties of the certified values were estimated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) [4] and include uncertainties relating to possible inhomogeneity (Section 4), instability (Section 5) and characterisation (Section 6). The materials are intended for the calibration or quality control of real-time PCR measurements to identify GTS 40-3-2 soya bean and/or quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs are available in glass bottles containing at least 1 g of dried soya bean seed powder, which were sealed under an atmosphere of argon. The minimum amount of sample to be used for extraction of the DNA is 200 mg. The CRMs were accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortium.JRC.D.2 - Standards for Innovation and sustainable Developmen

CERTIFICATION REPORT: The certification of different mass fractions of Bt11 in maize powder: Certified Reference Materials: ERM®-BF412ak, ERM®-BF412bk, ERM®-BF412ck, ERM®-BF412dk and ERM®-BF412ek

This report describes the production of a set of Certified Reference Materials (CRMs), ERM-BF412ak, bk, ck, dk and ek, which are certified for their Bt11 maize (unique identifier SYN-BTØ11-1) event mass fractions. These materials were produced following ISO Guide 34:2009 and are certified in accordance with ISO Guide 35:2006. Genetically modified (GM) maize seeds of the Bt11 event and seeds from a non GM maize variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM maize seed powder were prepared gravimetrically. The certified values were obtained from the gravimetric preparations, taking into account the water mass fractions of the two powder materials and the genetic purity with respect to the Bt11 maize. The certified values were confirmed by event-specific real-time PCR as an independent verification method (measurements were within the scope of accreditation to ISO/IEC 17025:2005). The uncertainties of the certified values were estimated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) and include uncertainties relating to possible inhomogeneity (Section 4), instability (Section 5) and characterisation (Section 6). The materials are intended for the calibration or quality control of real-time PCR measurements to identify Bt11 maize and quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs are available in glass bottles containing at least 1 g of dried maize seed powder, sealed under an atmosphere of argon. The minimum amount of sample to be used for extraction of the DNA is 200 mg.JRC.F.6-Reference Material

Certification Report - The certification of different mass fractions of DAS-81419-2 in soya seed powder: Certified Reference Materials ERM®-BF437a, ERM®-BF437b, ERM®-BF437c, ERM®-BF437d and ERM®-BF437e

This report describes the production of a set of Certified Reference Materials (CRMs) ERM BF437a, b, c, d and e, which are certified for their DAS-81419-2 mass fractions. This material was produced following ISO Guide 34:2009 and is certified in accordance with ISO Guide 35:2006. Genetically modified (GM) seeds of the soya event DAS-81419-2 and seeds from a non-GM soya variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM soya powder were prepared gravimetrically. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity with respect to the event DAS-81419-2 of the two powder materials and their water mass fractions. The certified values were confirmed by event-specific real-time PCR as an independent verification method (measurements were within the scope of accreditation to ISO/IEC 17025:2005). Uncertainties of the certified values were calculated in accordance with the Guide to the Expression of Uncertainty in Measurement (GUM) and include uncertainties relating to possible inhomogeneity (Section 4), instability (Section 5) and characterisation (Section 6). The materials are intended for the calibration or quality control of real-time PCR measurements to identify DAS-81419-2 soya and/or quantify its mass fraction. As with any reference material, they can also be used for establishing control charts or for carrying out validation studies. The CRMs are available in glass vials containing at least 1 g of dried soya seed powder which were sealed under atmosphere of argon. The minimum amount of sample to be used is 200 mg. The CRMs were accepted as European Reference Material (ERM®) after peer evaluation by the partners of the European Reference Materials consortiumJRC.D.2-Standards for Innovation and sustainable Developmen

Certification of Reference Materials of Potato Powder with Different Mass Fractions of the Event AM04-1020 - Certified Reference Materials ERM®-BF430 (ERM®-BF430a, ERM®-BF430b, ERM®-BF430c,ERM®-BF430d, ERM®-BF430e)

This report describes the processing and certification of five potato powder Certified Reference Materials (CRMs) containing different mass fractions of genetically modified (GM) AM04-1020 potato (ERM-BF430a,b,c,d,e). The materials were processed and certified in 2011 by the European Commission, Joint Research Centre, The Institute for Reference Materials and Measurements (IRMM, Geel, Belgium), according to the principles of ISO Guide 34JRC.D.2 - Standards for Innovation and sustainable Developmen

CERTIFICATION REPORT: The certification of different mass fractions of GMB151 in soya bean powder:

This report describes the production of ERM®-BF443, which are soya bean powder materials certified for the mass fraction of GMB151 (unique identifier BCS-GM151-6). These materials were produced and certified in accordance with ISO 17034:2016 and ISO Guide 35:2017. ERM BF443 was produced within the scope of ISO 17034 accreditation. Genetically modified (GM) soya bean seeds of the GMB151 event and seeds from a non-GM soya bean variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM soya bean seed powder were prepared gravimetrically. The CRMs are available in glass vials containing at least 1 g of dried soya bean powder, which were sealed under an atmosphere of argon. Between-unit homogeneity was quantified and stability during dispatch and storage was assessed in accordance with ISO Guide 35:2017. The minimum sample size for one measurement is 200 mg. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity of the base materials with respect to the GMB151 soya bean and their water mass fractions. The certified values were confirmed by event-specific real-time PCR (measurements produced by a laboratory with demonstrated competence and adhering to ISO/IEC 17025:2017. Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 and include uncertainties related to possible inhomogeneity, instability and characterisation. The materials are intended for the calibration or quality control of quantitative PCR measurements to identify GMB151 soya bean and quantify its mass fraction. As with any reference material, they can be used for establishing control charts or validation studies.JRC.F.6 - Reference Material

CERTIFICATION REPORT The certification of different mass fractions of GHB811 in cotton powder

This report describes the production of ERM®-BF442, which are cotton powder materials certified for the mass fraction of GHB811 (unique identifier BCS-GH811-4). These materials were produced and certified in accordance with ISO 17034:2016 and ISO Guide 35:2017. ERM BF442 was produced within the scope of ISO 17034 accreditation. Genetically modified (GM) cotton seeds of the GHB811 event and seeds from a non-GM cotton variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM cotton seed powder were prepared gravimetrically. The certified reference materials (CRMs) are available in glass vials containing at least 1 g of dried cotton powder, which were sealed under an atmosphere of argon. Between-unit homogeneity was quantified and stability during dispatch and storage was assessed in accordance with ISO Guide 35:2017. The minimum sample size for one measurement is 200 mg. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity of the base materials with respect to the GHB811 cotton and their water mass fractions. The certified values were confirmed by event-specific real-time polymerase chain reaction (PCR) (measurements performed under the scope of ISO/IEC 17025:2017 accreditation). Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 and include uncertainties related to possible inhomogeneity, instability and characterisation. The materials are intended for the calibration or quality control of quantitative PCR measurements to identify GHB811 cotton and quantify its mass fraction. As with any reference material, they can be used for establishing control charts or validation studies.JRC.F.6 - Reference Material

Certification of different mass fractions of DBN-09004-6 in soya bean seed powder: ERM®-BF441a, ERM®-BF441b, ERM®-BF441c, ERM®-BF441d and ERM®-BF441e

ERM®-BF441(a-e) are five soya seed powder materials certified for their mass fraction of DBN-09004-6 (unique identifier code DBN-Ø9ØØ4-6), produced within the scope of ISO 17034 accreditation. Genetically modified (GM) soya seeds of the DBN-09004-6 event and seeds from a non-GM soya variety were milled, sieved and dried to obtain GM and non-GM seed powders. Mixtures were prepared gravimetrically. Between-unit homogeneity was quantified and stability during transport and storage was assessed in accordance with ISO Guide 35:2017. The minimum sample size for one measurement is 200 mg. The certified values were obtained from the gravimetric preparations, taking into account the purity of the base materials and their water mass fractions. The certified values were confirmed by event-specific real-time PCR within the scope of accreditation to ISO/IEC 17025:2017. Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and include uncertainties related to possible inhomogeneity, instability and characterisation. The materials are intended for the calibration or quality control of methods for the identification and quantification of genetically modified DBN-09004-6 soya in food and feed.JRC.F.6 - Reference Material

The certification of different mass fractions of MIR162 in maize seed powder

This report describes the production of ERM®-BF446, maize seed powder materials certified for the mass fraction of MIR162 (unique identifier SYN-IR162-4). These materials were produced and certified in accordance with ISO 17034:2016 and ISO Guide 35:2017. ERM- BF446 was produced within the scope of ISO 17034:2016 accreditation. Genetically modified (GM) maize seeds of the MIR162 event and seeds from a non-GM maize variety were milled to obtain GM and non-GM seed powders with a similar particle size distribution. Mixtures of non-GM and GM maize seed powder were prepared gravimetrically. The certified reference materials (CRMs) are available in glass vials containing at least 1 g of dried maize seed powder, which were sealed under an atmosphere of argon. Between-unit homogeneity was quantified and stability during dispatch and storage was assessed in accordance with ISO Guide 35:2017. The minimum sample size for one measurement is 200 mg. The certified value was obtained from the gravimetric preparations, taking into account the genetic purity of the base materials with respect to the MIR162 maize and their water mass fractions. The certified values were confirmed by event-specific real-time polymerase chain reaction (PCR) (measurements performed adhering ISO/IEC 17025:2017). Uncertainties of the certified values were calculated in accordance with ISO 17034:2016 and ISO Guide 35:2017 and include uncertainties related to possible inhomogeneity, instability and characterisation.JRC.F.6 - Reference Material