Application of kaolin and italian natural chabasite-rich zeolitite to mitigate the effect of global warming in vitis vinifera l. Cv. sangiovese

High temperatures and the anomalous distribution of rainfall during the growing season may have a negative impact on grapevine yield and berry composition. In recent years, many studies have focused on the application of agronomical techniques to reduce the negative impact of heat waves on secondary metabolites such as phenols. In particular, treatments with kaolin have shown positive effects on reducing canopy temperatures, enhancing the accumulation of anthocyanins. In regard to the above, three treatments were evaluated: untreated control (C), kaolin (CAO), and chabasite-rich zeolitites (ZEO) applications on cv. Sangiovese in order to verify the cooling effects on leaves and bunches, and the impact on gas exchange, yield parameters, berry composition, and on both chemical and sensory notes of wine. Minerals were sprayed twice around the veraison on the entire canopy at a 3% concentration. The results showed that the application of the minerals was able to reduce the berry temperatures in both years of the trial as compared to the untreated control (C), without affecting vine gas exchange, yield, and soluble solid accumulation. Furthermore, the cooling effect determined an increase in anthocyanin on both the grapes and the wine. At testing, CAO and ZEO wines stood out regarding greater color intensity and were preferred by the judges

Foliar treatment of esca-proper affected vines with nutrients and bioactivators

Foliar treatment with nutrients and bioactivators was carried out in two vineyards affected with esca proper in 2004 and 2005. Changes in the foliar symptoms and in the quality of berries without lesions from treated symptomatic vines were assessed. Treated vines unexpectedly had a higher incidence and a greater severity of symptomatic leaves than untreated plants, most likely because physiological processes were stimulated by the treatments, possibly also because treatments facilitated the movement of toxins produced by the wood fungi of esca. However it cannot be excluded that the increase in foliar symptoms was due to the forced nutrition causing an imbalance between the various elements, and altering the mechanisms that vines use for the remission of foliar symptoms. This supposition seemed corroborated by the observation that treated vines diseased with esca proper had a weaker defense response than untreated diseased vines, and that treated diseased vines had lower levels of nitrogen and microelements, which are respectively involved in osmoregulation and as cofactors of enzymes involved in the defense response of the plant. The main quality parameters of berries without lesions from treated and untreated symptomatic vines were very similar

Innovative approach for a classic target: fragment screening on trypanothione reductase reveals new opportunities for drug design

Trypanothione reductase (TR) is a key factor in the redox homeostasis of trypanosomatid parasites, critical for survival in the hostile oxidative environment generated by the host to fight infection. TR is considered an attractive target for the development of new trypanocidal agents as it is essential for parasite survival but has no close homolog in humans. However, the high efficiency and turnover of TR challenging targets since only potent inhibitors, with nanomolar IC50, can significantly affect parasite redox state and viability. To aid the design of effective compounds targeting TR, we performed a fragment-based crystal screening at the Diamond Light Source XChem facility using a library optimized for follow-up synthesis steps. The experiment, allowing for testing over 300 compounds, resulted in the identification of 12 new ligands binding five different sites. Interestingly, the screening revealed the existence of an allosteric pocket close to the NADPH binding site, named the "doorstop pocket" since ligands binding at this site interfere with TR activity by hampering the "opening movement" needed to allow cofactor binding. The second remarkable site, known as the Z-site, identified by the screening, is located within the large trypanothione cavity but corresponds to a region not yet exploited for inhibition. The fragments binding to this site are close to each other and have some remarkable features making them ideal for follow-up optimization as a piperazine moiety in three out of five fragments

Impact of the environmental pollution on cardiovascular diseases: from epidemiological to molecular evidence

Environmental pollution poses a significant threat to human health, particularly concerning its impact on cardiovascular diseases (CVDs). This review synthesizes epidemiological and molecular evidence to elucidate the intricate relationship between environmental pollutants and CVDs. Epidemiological studies highlight the association between exposure to air, water, and soil pollutants and increased CVD risk, including hypertension, coronary artery disease, and stroke. Furthermore, molecular investigations unravel the underlying mechanisms linking pollutant exposure to CVD pathogenesis, such as oxidative stress, inflammation, endothelial dysfunction, and autonomic imbalance. Understanding these molecular pathways is crucial for developing targeted interventions and policy strategies to mitigate the adverse effects of environmental pollution on cardiovascular health. By integrating epidemiological and molecular evidence, this review provides insights into the complex interplay between environmental factors and CVDs, emphasizing the urgent need for comprehensive preventive measures and environmental policies to safeguard public health

Nutritional status of vines afected with esca proper

A vineyard of the cv. Trebbiano d’Abruzzo located in Abruzzo, Italy,  was monitored for more than ten years to distinguish healthy vines not only from vines with the visual leaf symptoms of esca, but also from those vines that were esca-infected but did not produce any visual symptoms for one or more growing seasons. In the period 2004–2006, leaves and berries were collected at four phenological growth stages from three groups of vines: healthy vines, infected vines showing esca symptoms, and infected vines that did not show symptoms. The macro and micro-elements of the leaves and berries, and the quality parameters of the must were determined. Esca did not seem to affect nutrient uptake in the vines. Nevertheless there were some differences in the nutrient levels of the leaves between healthy and diseased vines consistent with the degradation of the leaf blade caused by esca. Berries from symptomatic vines were less ripe at the time of harvesting and therefore had higher levels of mineral elements. These berries also had higher levels of nitrogen, which are thought to be associated with the defence response of diseased vines to esca, as are higher levels of iron in the leaves of diseased vines. The study confirmed earlier findings that fruit composition did not differ greatly between healthy and diseased-but-asymptomatic vines. In the three-year study period there were differences in the incidence of leaf symptoms and differences in nutrient levels attributable to fertiliser applications and rainfall. These differences suggested that the amount of mineral nutrients affected the onset of esca symptoms: a higher availability of nutrients in a growing season increased the proportion of diseased vines with symptoms and lowered the proportion of diseased vines without symptoms, whereas in a growing season with the lower levels of  water and potassium, the yield was reduced, but this was accompanied by an increase in the proportion of diseased vines without symptoms. It is suggested that a higher availability of nutrients for diseased vines lowers the resistance of these vines and, by improving the nutrition not only of the vines themselves but also of the esca fungi, increase fungal virulence, as a result of which there is a greater incidence of diseased vines showing leaf symptoms