Regulation of Topoisomerase IIa expression in humans : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry at Massey University, Palmerston North, New Zealand

In mammalian cells, the loss or down-regulation of tumour-suppressor genes and/or the mutation or overexpression of proto-oncogenes, whose products promote unregulated proliferation in cells, characterise the process of malignant transformation. This generates mitogenic signals that promote abnormal cell growth resulting in tumour progression. Topoisomerase IIα (topo IIα) is an enzyme present in elevated concentrations in highly proliferating cells due to the requirement for untwisting and unknotting of the DNA which is essential for replication. Because of this requirement, a number of anti-cancer drugs have been designed with topo IIα as their primary target. The effectiveness of these drugs however is limited by the development of resistance. One factor linked to drug resistance is the down-regulation of topo IIα at the transcription level. Expression of topo IIα appears to be regulated through various transcription factors with members of the Spl family having a major contribution. Previous work has shown down regulation of topo IIα can occur at the level of transcription. Nucleotide sequencing of the topo IIα promoter in drug-resistant cell lines has not revealed any mutations thus far. Three known proteins and one uncharacterised protein are capable of interacting with the proximal topo IIα promoter region. The uncharacterised protein may act as a co-activator or a co-repressor depending on the complement of transcription factors associated with the DNA in this region. Because drug resistant cell lines showed modulated expression of these transcription factors, it is important to identify the unknown protein and characterise its role in regulating topo IIα expression. This research aimed to identify the minimal binding site and DNA elements required for the uncharacterised protein to bind, as well as introduce mutations into this proximal region and examine their functional significance. The results of this study could provide insights into the molecular mechanisms responsible for the development of drug resistance, contributing to more efficient and effective methods for the treatment of cancer

Sensory quality of scab-resistant apple cultivars

Twenty-two scab-resistant apple cultivars were harvested in autumn 1999 and evaluated for sensory quality the following October, November and December. Multivariate analysis was effective in describing the comblex relationships and variabillity among the numerous attributes used to characterise apple quality. Crispness, mealiness, skin toughness, apple flavour, sweetness, unripe flavour and overripe flavour were informative attributes describing the variation in the sensory quality. Texture attributes, apple flavour and overripe flavour were affected by storage. Extended storage resulted in an increase in mealiness and overripe flavour and a decrease in crispness, juiciness and apple flavour. Many potential scab-resistant cultivars were suitable for consumption in October: 'Dayton', 'Primicia', 'Retina' and 'Realka'; in November: 'Merlijn', 'Saturn', 'Initial', 'Realka', 'Rajka' and 'Rubinola'; and in December: 'Otava', 'Ecolette', 'Rejka', 'Rubinola', 'Delorina', 'Initial' and 'Resista' and 'Topaz'. This study gives a sensory sharacterisation of scab-resistant cultivars as an indicator of suitability for commercial growing and marketing of low-input apple cultivars

Multi Agent Modelling: Evolution and Skull Thickness in Hominids

Within human evolution, the period of Homo Erectus is particularly interesting since in this period, our ancestors have carried thicker skulls than the species both before and after them. There are competing theories as to the reasons of this enlargement and its reversal. One of these is the theory that Homo Erectus males fought for females by clubbing each other on the head. The other one says that due to the fact that Homo Erectus’ did not cook their food at all, they had to have strong jaw muscles attached to ridges on either side of the skull which prohibited brain and skull growth but required the skull to be thick. The re-thinning of the skull on the other hand might be due to the fact that a thick skull provided poor cooling for the brain or that as hominids started using tools to cut their food and using fire to cook it, they did not require the strong jaw muscles anymore and this trait was actually selected against since the brain had a tendency to grow and the ridges and a thick skull were preventing this. In this paper we simulated both the fighting and the diet as ways in which the hominid skull grew thicker. We also added other properties such as cooperation, selfishness and vision to our agents and analyzed their changes over generations. Keywords: Evolution, Skull Thickness, Hominids, Multi-Agent Modeling, Genetic Algorithm

Compact printed multiband antenna with independent setting suitable for fixed and reconfigurable wireless communication systems

This is the author's accepted manuscript. The final published article is available from the link below. Copyright @ 2012 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other users, including reprinting/ republishing this material for advertising or promotional purposes, creating new collective works for resale or redistribution to servers or lists, or reuse of any copyrighted components of this work in other works.This paper presents the design of a low-profile compact printed antenna for fixed frequency and reconfigurable frequency bands. The antenna consists of a main patch, four sub-patches, and a ground plane to generate five frequency bands, at 0.92, 1.73, 1.98, 2.4, and 2.9 GHz, for different wireless systems. For the fixed-frequency design, the five individual frequency bands can be adjusted and set independently over the wide ranges of 18.78%, 22.75%, 4.51%, 11%, and 8.21%, respectively, using just one parameter of the antenna. By putting a varactor (diode) at each of the sub-patch inputs, four of the frequency bands can be controlled independently over wide ranges and the antenna has a reconfigurable design. The tunability ranges for the four bands of 0.92, 1.73, 1.98, and 2.9 GHz are 23.5%, 10.30%, 13.5%, and 3%, respectively. The fixed and reconfigurable designs are studied using computer simulation. For verification of simulation results, the two designs are fabricated and the prototypes are measured. The results show a good agreement between simulated and measured results

Musical direction of student end of year concert 2018

Musical direction of Wintec Song Arranging class of 2018 End Of Year Concert. A public performance of students' final arrangements from the semester 1 paper. The un-themed program, a selection of popular tunes from 1960s - 2018 was devised to offer students the opportunity to hear their arrangements performed live by a small orchestra comprised of piano, guitar, bass and drums, trumpet, alto sax, tenor sax and trombone and a string section of 3 1st violins, 2 second violins, 2 violas and 2 cellos. Prior to this performance the students were only able to hear their work played as a computer-generated audio file (far from ideal) so the concert provided an invaluable 'real world' experience. The performance was devised as a professional experience - the tutor acting as Producer and Musical Director/Conductor, the students becoming arrangers who also prepared their scores and instrumental parts ready for printing and performance within real-time deadlines to emulate a real-world work experience

Performance of original New Zealand themed choral work 'Summer Christmas' composed by Wayne Senior

'Summer Christmas' was chosen to be performed in Camerata Chamber Choir's November 14, 2018 Christmas program which was subsequently titled 'Summer Christmas’ About the work: (dur. 6:20) with music and lyrics composed by Wayne Senior, speaks in evocative settings of things relevant to New Zealand referencing beach scenes, the warmth of the summer sun, native birds, the mighty Kauri and ‘our very own Christmas tree’ the iconic Pohutukawa, whilst drawing comparisons with winter Christmas in ‘cooler climes’ (e.g. 'no chestnuts roasting on an open fire’ and a new setting of ‘O Magnum Mysterium’) closing with a choral representation of church bells. My original text is a combination of English, Te reo Māori and Latin, incorporating translations by Honi Rawhiti (George Lusty). The setting of ‘O Magnum Mysterium’ is in Latin with alternating lines in English and Te Reo Māori. Scored for choir and piano, it is envisioned that an enhanced accompaniment will be added for possible future performances with orchestral resources if and when required. Also that it will be re-scored in other versions for young students' choir and a less advanced version for community choir

Robust H∞ filtering for markovian jump systems with randomly occurring nonlinearities and sensor saturation: The finite-horizon case

This article is posted with the permission of IEEE - Copyright @ 2011 IEEEThis paper addresses the robust H∞ filtering problem for a class of discrete time-varying Markovian jump systems with randomly occurring nonlinearities and sensor saturation. Two kinds of transition probability matrices for the Markovian process are considered, namely, the one with polytopic uncertainties and the one with partially unknown entries. The nonlinear disturbances are assumed to occur randomly according to stochastic variables satisfying the Bernoulli distributions. The main purpose of this paper is to design a robust filter, over a given finite-horizon, such that the H∞ disturbance attenuation level is guaranteed for the time-varying Markovian jump systems in the presence of both the randomly occurring nonlinearities and the sensor saturation. Sufficient conditions are established for the existence of the desired filter satisfying the H∞ performance constraint in terms of a set of recursive linear matrix inequalities. Simulation results demonstrate the effectiveness of the developed filter design scheme.This work was supported in part by the National Natural Science Foundation of China under Grants 61028008, 60825303, and 61004067, National 973 Project under Grant 2009CB320600, the Key Laboratory of Integrated Automation for the Process Industry (Northeastern University) from the Ministry of Education of China, the Engineering and Physical Sciences Research Council (EPSRC) of the U.K., under Grant GR/S27658/01, the Royal Society of the U.K., and the Alexander von Humboldt Foundation of Germany

Do nutrient uptakes and grain yields differ between spring barley varieties grown for organic farming as mono-crop and in mixture: Characteristics of spring barley varieties for organic farming

Manure application increased yield. Hence the study was conducted under the conditions of moderate nutrient stress and in all probability nitrogen stress. Under this condition nutrient uptakes, biomass production and grain yields did vary between Otira, Orthega, Landora, Brazil, Svani, NK96-300, Mixture 1 (Otira, Orthega, Landora) and Mixture 2 (Brazil, Svani and NK96-300). Interaction between variety and soil fertility treatments were also significant. These findings open new possibilities for selection or plant breeding for genotypes with improved capability of nutrient acquisition from soils at different soil fertility levels e.g. in organic farming. Cropping of barley varieties in mixtures seems to increase (stimulate) nutrient uptakes (N, P, S, K, Mg, Ca, Mn, Zn, Cu and Mo) and biomass production

Pairwise Check Decoding for LDPC Coded Two-Way Relay Block Fading Channels

Partial decoding has the potential to achieve a larger capacity region than full decoding in two-way relay (TWR) channels. Existing partial decoding realizations are however designed for Gaussian channels and with a static physical layer network coding (PLNC). In this paper, we propose a new solution for joint network coding and channel decoding at the relay, called pairwise check decoding (PCD), for low-density parity-check (LDPC) coded TWR system over block fading channels. The main idea is to form a check relationship table (check-relation-tab) for the superimposed LDPC coded packet pair in the multiple access (MA) phase in conjunction with an adaptive PLNC mapping in the broadcast (BC) phase. Using PCD, we then present a partial decoding method, two-stage closest-neighbor clustering with PCD (TS-CNC-PCD), with the aim of minimizing the worst pairwise error probability. Moreover, we propose the minimum correlation optimization (MCO) for selecting the better check-relation-tabs. Simulation results confirm that the proposed TS-CNC-PCD offers a sizable gain over the conventional XOR with belief propagation (BP) in fading channels.Comment: to appear in IEEE Trans. on Communications, 201

USE OF A RAT MODEL TO ELUDICATE IMPACT OF ORGANIC FOOD ON HEALTH

Ingredients (potatoes, carrots, peas, green kale, apples, and rapeseed oil) were grown according to three different cultivation systems (“Organic”, low input of fertilizer without pesticides; “Minimally fertil-ised”, low input of fertilizer and high input of pesti-cides, “Conventional”, high input of fertilizer and high input of pesticides). Three iso-energetic and iso-nitrogeneous diets were composed of equal propor-tions of the ingredients originating from each of the cultivation systems, and the diets were investigated with respect to several physiological responses and biomarkers of health using a rat model. In addition, the diets were tested in a food preference test using the same type of rats, but another generation. In both experiments, the diets were optimized according to the nutritional requirements of reproducing rats, except for a high content of fat. Most of the measured variables (biomarkers of health) showed no differ-ences between the experimental diets, however, some differences between dietary treatments were obtained, which were in favour of the “organic” diet contrasted with the “conventional” diet. The prefer-ence test showed a significant interaction between diet choice and mother’s diet. However, the results obtained from the present study cannot be extrapo-lated to all organic and conventional cropping sys-tems, mainly because crops were grown only in one replication. Thus, it is of outmost importance that future investigations on the effect of organic food in relation to human health and well-being should be based on well-defined and controlled food produce system with replication