Vitrification of human immature oocytes before and after in vitro maturation: a review

The use of immature oocytes subjected to in vitro maturation (IVM) opens interesting perspectives for fertility preservation where ovarian reserves are damaged by pathologies or therapies, as in PCO/PCOS and cancer patients. Human oocyte cryopreservation may offer some advantages compared to embryo freezing, such as fertility preservation in women at risk of losing fertility due to oncological treatment or chronic disease, egg donation and postponing childbirth. It also eliminates religious and/or other ethical, legal, and moral concerns of embryo freezing. In addition, a successful oocyte cryopreservation program could eliminate the need for donor and recipient menstrual cycle synchronization. Recent advances in vitrification technology have markedly improved the oocyte survival rate after warming, with fertilization and implantation rates comparable with those of fresh oocytes. Healthy live births can be achieved from the combination of IVM and vitrification, even if vitrification of in vivo matured oocytes is still more effective. Recently, attention is given to highlight whether vitrification procedures are more successful when performed before or after IVM, on immature GV-stage oocytes, or on in vitro matured MII-stage oocytes. In this review, we emphasize that, even if there are no differences in survival rates between oocytes vitrified prior to or post-IVM, reduced maturation rates of immature oocytes vitrified prior to IVM can be, at least in part, explained by underlying ultrastructural and biomolecular alterations

Economic burden of diverticular disease : an observational analysis based on real world data from an Italian region

INTRODUCTION: Diverticular disease (DD), a herniation of the colonic mucosa through the muscle layer, covers a wide variety of conditions associated with the presence of diverticula in the colon. The most serious form is an acute episode of diverticulitis, which can lead to hospitalization and surgery with various types of consequences. The main aim of this study is to evaluate the economic burden of hospitalizations arising from acute episodes of diverticulitis using data from the administrative databases used in the Marche region in Italy and, as a secondary objective of this real-world data analysis, to study patient outcome variables following initial hospitalization for diverticulitis. METHOD: A deterministic linkage was performed at individual user level between the different administrative sources of the Marche region through anonymous ID number for a period of analysis between 1 January, 2008 and 31 December, 2014. We enrolled all patients with at least one hospitalization for "diverticulitis of the colon without mention of haemorrhage" (ICD-9-CM code 562.11) or "diverticulitis of the colon with haemorrhage" (ICD-9-CM code 562.13) as primary or secondary diagnosis. For each patient we assessed the cost of hospitalization, of medicines and of specialist services considering a time-scale of one year or cohort analysis 365days after first admission. RESULTS: The total number of residents in the Marche region who had at least one hospitalization for diverticulitis in the period 2008-2014 was 2987 (427 patients a year, corresponding to about 35 patients per 100,000 adult residents); the total number of admissions was 3453 (just over 490 a year). The direct healthcare costs incurred by the Marche region for episodes of diverticulitis in 2008-2014 amounted to approximately €11.4 million (€1.6 million a year), of which €10.9 million (95.5%) for the hospitalizations, € 246,000 (2.1%) for pharmaceutical treatment and €270,000 (2.4%) for specialist outpatient services. The average annual cost per patient was €3826, of which €3653 was for hospitalization, while pharmaceutical expenditure and specialist services accounted for €83 and €90, respectively. The cohort of patients undergoing a first admission for diverticulitis between 2010 and 2013 was made up of 1729 people (54.4% women, mean age 68.9 years), of whom 1500 (86.8%) did not undergo surgery while in hospital. Hospital mortality, recorded only for the over-65 age class, averaged 1.2%; for patients not receiving surgery during the initial hospitalization it was 0.5%, reaching 5.2% in patients undergoing surgery. The percentage of patients with one or more readmissions for diverticulitis within a year of the first was on average 7.8% and in 48% of cases this resulted in surgery. CONCLUSIONS: Our study is the first analysis in Italy to use real-world data to measure the financial impact of diverticular disease. Assuming that the diagnostic and therapeutic behaviour identified in the Marche region could be representative of the situation nationwide, the estimated annual number of hospitalizations in Italy for acute episodes of diverticulitis is 19,000. The total amount of economic resources needed to treat patients suffering from acute episodes of diverticulitis is estimated at €63.5 million a year

Novel Ag/Kaolin Nanocomposite as Adsorbent for Removal of Acid Cyanine 5R from Aqueous Solution

Ag/kaolin nanocomposite was prepared by reduction of Ag+ ion with ethanol at alkaline condition on kaolin surface. Nanocomposite was characterized by FTIR, XRD, TEM, and BET methods. Results showed the Ag/kaolin composite has particle size 50 nm. The surface area was increased from kaolin to Ag/kaolin from 1.0215 to 7.409 m2 g−1, respectively. Ag/kaolin nanocomposite was used for adsorption of acid cyanine 5R (AC5R) from aqueous solution. The effect of parameters such as contact time, pH, and mass of nano composite has been investigated. The maximum percentage of adsorption of AC5R was found at pH 3 and contact time of 60 min. The higher percentage removal of AC5R by Ag/kaolin than kaolin can be attributed to catalytic activity of Ag on the surface of kaolin. The experimental data was fitted by pseudo-second-order kinetic model. The adsorption isotherm data could be well interpreted by Langmuir isotherm model. From the results of thermodynamic study, the adsorption process of AC5R onto Ag/kaolin nanocomposite was spontaneous and endothermic process. The process is clean and safe for purifying of water pollution

The situation of working environment will affect the job performance of employees at Perbadanan Kemajuan Negeri Melaka / Suraiti Shahedi

Positive working environment has been identified as a driver of performance. It is important because employee valuations of the environment will influence their job performance. Nowadays, numbers of civil servants that suffer from workplace situation have increase. So that it is important for management to manage their working environment and improve weaken area. This study is a research of 'The Situation of Working Environment Will Affect the Job Perfomance of Employees at Perbadanan Kemajuan Negeri Melaka'. The study utilize questionnaire as the instrument for collecting data. Questionnaires have been distributed to 40 employees from all departments at the organization to do the research. In this study, researcher has identifying the types of working environment at Perbadanan Kemajuan Negeri Melaka and the level of job performance of the employees. Researchers will test the relationship between working environment and the job performance in this study. Researchers have used the Statistical Package for Social Science (SPSS) Program Student Version. From the analysis researchers have identify that there is significant relationship between working environment and job performance of employees at Perbadanan Kemajuan Negeri Melaka

Lead-time-based freight routing in multi-modal networks considering the Physical Internet

This paper addresses the problem of optimizing the transport of goods in the Physical Internet (PI) framework in a multi-modal setting using a multi-objective mixed-integer linear programming (MILP) approach. The model is specifically designed to meet the requirements related to modular shipments and PI-hubs, and in particular, determines the allocation of modular shipments to each transport mode in an intermodal setting. In doing so, parallel direct connection via road, the delivery times and the transportation costs are minimized. The model is applied to a numerical case study, to test its effectiveness to enhance freight transport efficiency within the PI framework, by exploiting, in particular, all the capacities of the available vehicles. In addition, a sensitivity analysis is conducted on some model parameters, to test its reaction to changes in the supply system and in the objective priorities. Results show that all the shipments are effectively transported between the origin and the destination terminals, they are divided into modules when necessary, and the selected transport modes, allocation strategy, and delivery times vary accordingly to the objective priorities

A Scenario-Based Approach for Assessing the Hydrological Impacts of Land Use and Climate Change in the Marboreh Watershed, Iran

AbstractIn separate analyses of the impacts of land use change and climate change, a scenario-based approach using remote sensing and hydro-climatological data was developed to assess changes in hydrological indices. The data comprised three Landsat TM images (1988, 1998, 2008) and meteorological and hydrological data (1983–2012) for the Aligudarz and Doroud stations in the Marboreh watershed, Iran. The QUAC module and supervised classification maximum likelihood (ML) algorithm in ENVI 5.1 were used for remote sensing, the SWAT model for hydrological modelling and the Mann-Kendall and t test methods for statistical analysis. To create scenarios, the study period was divided into three decades (1983–1992, 1993–2002, 2003–2012) with clearly different land use/land cover (LULC). After hydrological modelling, 10 hydrological indices related to high and low flow indices (HDI and LDI) were analysed for seven scenarios developed by combining pre-defined climate periods and LULC maps. The major changes in land use were degradation of natural rangeland (−18.49%) and increasing raid-fed farm area (+16.70%) and residential area (+0.80%). The Mann-Kendall test results showed a statistically significant (p Abstract In separate analyses of the impacts of land use change and climate change, a scenario-based approach using remote sensing and hydro-climatological data was developed to assess changes in hydrological indices. The data comprised three Landsat TM images (1988, 1998, 2008) and meteorological and hydrological data (1983–2012) for the Aligudarz and Doroud stations in the Marboreh watershed, Iran. The QUAC module and supervised classification maximum likelihood (ML) algorithm in ENVI 5.1 were used for remote sensing, the SWAT model for hydrological modelling and the Mann-Kendall and t test methods for statistical analysis. To create scenarios, the study period was divided into three decades (1983–1992, 1993–2002, 2003–2012) with clearly different land use/land cover (LULC). After hydrological modelling, 10 hydrological indices related to high and low flow indices (HDI and LDI) were analysed for seven scenarios developed by combining pre-defined climate periods and LULC maps. The major changes in land use were degradation of natural rangeland (−18.49%) and increasing raid-fed farm area (+16.70%) and residential area (+0.80%). The Mann-Kendall test results showed a statistically significant (p < 0.05) decreasing trend in rainfall and flow during 1983–2012. In the scenarios evaluated, hydrological index trends were more sensitive to climate change than to LULC changes in the study area. Low flow indices were more affected than high flow indices in both land use and climate change scenarios. The results show little impact of land use change and indicate that climate change is the main driver of hydrological variations in the catchment. This is useful information in outlining future strategies for sustainable water resources management and policy decision-making in the Marboreh watershed