Synthesis of Mn0.2Zn0.8Fe2O4 particles by high energy ball milling and their applications

Ultra fine Mn0.2Zn0.8Fe2O4 magnetic particles are developed by high energy ball milling technique and investigated for physical properties. The crystalline phase, crystallite size, surface morphology, metal oxide bonding and porosity of these magnetic particles are analyzed. The porosity increases on increasing the grinding period due to decrease in the particle size and crystallinity reduces. The IR spectra measured in the range of 4000-400 cm-1 exhibit symmetric stretching mode of (FeO4) and (ZnO4) tetrahedral at 669.7 cm-1 and 545.6 cm-1. The lattice strain induced by ball milling process has been determined. The porosity plays an important role in chemisorption and physisorption of species on the sites of the particles. This property of these particles has been exploited for the applications of humidity sensor

To boost or not? Serum anti-COVID IgG in double vaccinated MBBS students: a cross-sectional study

Background: Aim of the study was to estimate the prevalence of sero-positivity i.e. serum anti-covid IgG >1.4 AU/ml in MBBS students of our Institute. Methods: This study was carried out on 75 MBBS students of our institute (10% of 750 MBBS students of Amaltas institute of Medical Sciences Dewas M.P. India) during period of August 2022 to November 2022. All serum anti-covid IgG titres above 1.4 AU/ml were considered sero-positive and the prevalence of sero-positivity was calculated. MBBS students were asked to fill out a questionnaire regarding their clinical history of testing positive for covid, exposure to COVID-19 patients, their covid appropriate behavior, etc. After obtaining informed consent, 4 ml venous blood samples were collected for determining serum anti-covid IgG titre on Chemiluminescence analyzer. 75 MBBS students divided into 2 groups – those who completed between 6-9 months and those who have completed more than 9 months after the 2nd dose of vaccine. The mean serum anti-covid IgG titre was compared. Results: The prevalence of sero-positivity was 94.66% calculated in MBBS students of our institute. The total antibody sero-positivity was higher in males (98.11%) than in females (86.36). The duration after 2nd dose of vaccine; over the time 6-9 months serum anti-covid IgG titre significantly high but after more than 9 months serum anti covid IgG titre significantly decline (p<0.001) and significant correlation was found between duration after 2nd dose of vaccine and serum anti-covid IgG titres (p<0.001). Conclusions: This study; strongly recommended the need for booster doses for long-duration protection against COVID-19 re-infection and its emerging new variants

Management of Health Through Vajrasana: A Comprehensive Guide

Vajrasana, also known as the "Thunderbolt Pose," is one of the most revered postures in Yoga. It is frequently practiced for its numerous health benefits, including improved digestion, enhanced circulation, and mental clarity. Beyond the physical benefits, Vajrasana has been embraced in Ayurvedic practices for its therapeutic effects on the body and mind. This comprehensive guide delves into the role of Vajrasana in health management, exploring its alignment with Ayurvedic principles and providing an in-depth analysis of its advantages. The article highlights its significance in strengthening the digestive system, calming the mind, and promoting overall well-being, referencing ancient Ayurvedic texts and modern-day interpretations

Случай раннего выявления метастазов в бедренной кости у больного раком полового члена

Background. Bone metastasis is very common in the advanced stage of numerous carcinomas. In penile carcinoma, lymph nodes metastasis is somehow common but it is very rare reported to be secondary from penile cancer. till the date, there are only few cases of penis carcinoma reported bone metastasis in literature worldwide.Case Presentation. Herein, We presented a 51-year-old Nepalese male with squamous cell carcinoma of penis. computed tomography (ct) scan of the patient revealed that there was carcinoma involving glans penis and precure with bilateral external & internal inguinal lymphadenopathies. After then, the patient was under gone for partial penectomy and bilateral inguinal lymphadenectomy and complete 6-cycle chemotherapy. After one year of treatment, patient developed thigh pain and headache and he advised to have magnetic Resonance imaging (mRi) of brain, 99mTc-MDP whole body bone scan and ct scan of pelvis and thigh. The examination report reveals that there was a sclerotic change in vertex of skull bone and moderate 99mTc-MDP uptake in right proximal shaft of femur just below the neck d/d metastasis. The histopathological examination of the true cut biopsy taken from the lesion of the femur showed metastatic keratinizing squamous cell carcinoma which is rare case of femoral shaft bone metastasis secondary from penile carcinoma. Then patient was sent for surgical reconstruction of femur. Based on the case studies review femur shaft bone metastasis from penile cancer is extremely rare.Conclusion. The best of our knowledge; this is the first early detected bone metastases to shaft of the femur in a patient with penile cancer. early diagnosis helps to radical treatment as well as palliative treatment. surgery is the preferred option of the treatments, especially for metastatic foci in the long bones.Аннотация Метастазы в кости часто встречаются на поздних стадиях разных злокачественных новообразований. Для рака полового члена характерно лимфогенное метастазирование, костные метастазы встречаются очень редко. На данный момент в мировой литературе зарегистрировано лишь несколько случаев рака полового члена с метастазами в кости.Описание клинического случая. Мы представляем описание клинического случая плоскоклеточного рака полового члена у 51-летнего мужчины из Непала. Компьютерная томография (КТ) выявила рак полового члена, локализованный на головке и крайней плоти с двусторонней паховой лимфаденопатией. Пациенту выполнена частичная пенэктомия, двусторонняя паховая лимфаденэктомия, проведено 6 курсов адъювантной химиотерапии. Через один год после завершения лечения у пациента появились боли в бедре и головная боль, рекомендована МРТ головного мозга, сканирование костей скелета с 99mTc-MDP и КТ таза и бедра. Обследование показало наличие склеротических изменений в теменной кости черепа и умеренное накопление 99mTc-MDP в проксимальном отделе правом бедренной кости ниже шейки. Гистологическое исследование биоптата, взятого из очага в бедренной кости, выявило метастаз ороговевающего плоскоклеточного рака, что является редким случаем метастазирования рака полового члена в диафиз бедренной кости. Пациент был направлен на хирургическую реконструкцию бедренной кости. Согласно обзору клинических исследований, метастазы в диафизе бедренной кости при раке полового члена встречаются крайне редко.Заключение. Представлен первый случай раннего выявления костных метастазов в диафизе бедренной кости у пациента с раком полового члена. Ранняя диагностика помогает как радикальному, так и паллиативному лечению. Хирургическое вмешательство является предпочтительным вариантом лечения, особенно при метастатических очагах в длинных костях

Innovations in Pharmaceutical Manufacturing through Edge AI for Process Optimization and Standard Compliance

EdgeAI integrates artificial intelligence with edge computing, enabling real-time decision-making in industrial applications. In pharmaceutical manufacturing, EdgeAI enhances process efficiency, predictive maintenance, and quality control by analyzing data locally, reducing cloud dependency, and improving system responsiveness. This paper examines the role of EdgeAI and embedded systems in optimizing pharmaceutical production, ensuring regulatory compliance, and minimizing downtime. A review of recent advancements highlights how AI-driven edge computing enables rapid fault detection, automated drug formulation monitoring

Evaluation of potential role of R-loop and G-quadruplex DNA in the fragility of c-MYC during chromosomal translocation associated with Burkitt\u27s lymphoma

t(8;14) translocation is the hallmark of Burkitt\u27s lymphoma and results in c-MYC deregulation. During the translocation, c-MYC gene on chromosome 8 gets juxtaposed to the Ig switch regions on chromosome 14. Although the promoter of c-MYC has been investigated for its mechanism of fragility, little is known about other c-MYC breakpoint regions. We have analyzed the translocation break points at the exon 1/intron 1 of c-MYC locus from patients with Burkitt\u27s lymphoma. Results showed that the breakpoint region, when present on a plasmid, could fold into an R-loop confirmation in a transcription-dependent manner. Sodium bisulfite modification assay revealed significant single-strandedness on chromosomal DNA of Burkitt\u27s lymphoma cell line, Raji, and normal lymphocytes, revealing distinct R-loops covering up to 100 bp region. Besides, ChIP-DRIP analysis reveals that the R-loop antibody can bind to the breakpoint region. Further, we show the formation of stable parallel intramolecular G-quadruplex on non-template strand of the genome. Finally, incubation of purified AID in vitro or overexpression of AID within the cells led to enhanced mutation frequency at the c-MYC breakpoint region. Interestingly, anti-γH2AX can bind to DSBs generated at the c-MYC breakpoint region within the cells. The formation of R-loop and G-quadruplex was found to be mutually exclusive. Therefore, our results suggest that AID can bind to the single-stranded region of the R-loop and G4 DNA, leading to the deamination of cytosines to uracil and induction of DNA breaks in one of the DNA strands, leading to double-strand break, which could culminate in t(8;14) chromosomal translocation