Extraction, Isolation and Identification of Antimicrobial Substances from Bacillus amyloliquefaciens CMN1308

Four separation methods of antimicrobial substances produced by CMN1308 (Bacillus amyloliquefaciens) were evaluated and selected according to number of antimicrobial substances and its activity in vitro. The results showed that extraction by acid precipitation of the fermentation supernatant of CMN1308 was the best with a diameter of inhibition zone of pathogen fungi P. expansum of 12.3 mm in a laboratory bioassay. Applying a silica thin layer chromatography (TLC), SDS-PAGE and other separation technologies we isolate antimicrobial substances, and the separated band were cut off for mass spectrometry analysis. The TLC of crude extract of CMN1308 show a topical band corresponding with the surfactin standard (Rf value =0.75), proved that the strain CMN1308 can produce this surface active compound. The mycoprotein extracted from CMN1308 was separated by Tricine-SDS-PAGE modified with the addition of urea in the separation gel. After mass spectrometric analysis and protein characterization, the isolated mycoprotein showed a maximum ion peak at M/Z of 2679 and molecular weight of 29.5 kDa, matching with protein flagellin. The extracellular antimicrobial protein of strain CMN1308 display four bands after urea-Tricine-SDS-PAGE, but after mass spectrometry analysis only two bands were identified. Band “A” with a maximum ion peak at M/Z of 1926 and molecular weight of 49.8 kDa, aligned with NCBI database, matching with DLDH (dihydrolipoamide dehydrogenase enzyme). Band “D” show the maximum ion peak at M/Z of 2936 and molecular weight of 22.4 kD, matching with a chitin binding protein. Thus, the strain CMN1308 has the potential to be developed as a commercial biological control agent for chestnut common pathogenic fungi

Preparation and Physicochemical Properties of Microcapsules Loaded with Selenium-Enriched Peptides and VE

A microcapsule system for the co-encapsulation of selenium-enriched peptides, being hydrophilic, and vitamin E (VE), being lipophilic, was developed using a combination of emulsification and freeze-drying. The effects of wall material concentration, selenium-enriched peptide content, and VE content on the encapsulation efficiency was investigated by single-factor experiments. Fourier transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM) showed that the selenium-enriched peptides and VE were effectively encapsulated in the microcapsules, and the microcapsules possessed good water dispersibility and maintained its double emulsion structure after reconstitution. The results of thermal stability analysis and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) cation radical scavenging assay confirmed that compared with selenium-enriched peptides, the microcapsules had higher thermal stability and antioxidant activity. Furthermore, electronic nose analysis showed that the microcapsule system possessed a good masking effect on the odor of selenium-enriched peptides. In vitro simulated digestion experiments showed that microencapsulation enhanced the stability of selenium-enriched peptides in simulated gastric juice and lowered the retention rate in simulated intestinal fluid; selenium-enriched peptides were effectively released from the microcapsules. This study will provide a theoretical basis for the development of selenium-enriched functional foods and nutritional supplements

Phragmites australis

Aquatic plants play an essential role and are effective in mitigating lake eutrophication by forming complex plant-soil system and retaining total nitrogen (TN) and phosphorus (TP) in soils to ultimately reduce their quantities in aquatic systems. Two main vegetation types (Phragmites australis community and P. australis + Typha latifolia community) of Qin Lake wetland were sampled in this study for the analysis of TN and TP contents and reserves in the wetland soils. The results showed that (1) the consumption effect of Qin Lake wetland on soluble N was much more significant than on soluble P. (2) The efficiency of TN enrichment in wetland soil was enhanced by vegetation covering of P. australis and T. latifolia. (3) Wetland soil P was consumed by P. australis community and this pattern was relieved with the introduction of T. latifolia. (4) According to the grey relativity analysis, the most intensive interaction between plants and soil occurred in summer. In addition, the exchange of N in soil-vegetation system primarily occurred in the 0–15 cm soil layer. Our results indicated that vegetation covering was essential to the enrichment of TN and TP, referring to the biology-related fixation in the wetland soil

A Fast Method for Calculation of Marine Gravity Anomaly

Gravity data have been playing an important role in marine exploration and research. However, obtaining gravity data over an extensive marine area is expensive and inefficient. In reality, marine gravity anomalies are usually calculated from satellite altimetry data. Over the years, numerous methods have been presented for achieving this purpose, most of which are time-consuming due to the integral calculation over a global region and the singularity problem. This paper proposes a fast method for the calculation of marine gravity anomalies. The proposed method introduces a novel scheme to solve the singularity problem and implements the parallel technique based on a graphics processing unit (GPU) for fast calculation. The details for the implementation of the proposed method are described, and it is tested using the geoid height undulation from the Earth Gravitational Model 2008 (EGM2008). The accuracy of the presented method is evaluated by comparing it with marine shipboard gravity data. Its efficiency is demonstrated through comparison with the conventional sequential method. The tests demonstrate that the proposed method can be employed for accurately calculating marine gravity anomalies and provides an advantage on computational efficiency.</jats:p

Core–shell Pd–P@Pt–Ni nanoparticles with enhanced activity and durability as anode electrocatalyst for methanol oxidation reaction

The Pd–P@Pt–Ni core–shell nanoparticles consist of an amorphous core and a low-crystalline shell. They exhibit the excellent catalytic properties in MOR owing to the double synergistic effects from the core and the nickel species in the shell.</jats:p

Antipsychotic-Related Risks of Type 2 Diabetes Mellitus in Enrollees With Schizophrenia in the National Basic Public Health Service Program in Hunan Province, China

BackgroundAntipsychotics contribute to the development of type 2 diabetes mellitus (T2DM) in individuals with schizophrenia. However, the extent of the relationship between antipsychotic use and T2DM varies in different settings, and the magnitude of the drug-specific effects fluctuates widely. This study aimed to explore the association of T2DM with antipsychotic use among enrollees with schizophrenia in China's National Basic Public Health Service Program (NBPHSP) and the drug-specific relationship with T2DM among patients receiving antipsychotic monotherapy.MethodsWe recruited diabetes-free patients with schizophrenia who were enrolled in the NBPHSP of Hunan Province from October 2009 to December 2018. The participants were classified into the following three groups: regular antipsychotic use, intermittent antipsychotic use, and antipsychotic-free groups. The patients were followed up until they received a T2DM diagnosis or until April 2019. Cox regression models were constructed to calculate the overall and drug-specific hazard ratios (HRs) to determine the antipsychotic–T2DM relationship. Interactive and subgroup analyses were performed to assess the heterogeneity of the effects across subgroups.ResultsA total of 122,064 NBPHSP enrollees with schizophrenia were followed up for 1,507,829 cumulative person-years, and 2,313 (1.89%) patients developed T2DM. Patients who regularly and intermittently used antipsychotics had 117% (HR: 2.17, 95% CI: 1.83–2.57) and 53% (HR: 1.53, 95% CI: 1.23–1.90) higher risks of developing T2DM than antipsychotic-free patients, respectively. Regarding monotherapy, the T2DM risk increased by 66, 80, 62, and 64% after the regular use of clozapine, risperidone, chlorpromazine, and perphenazine, respectively. In addition, the antipsychotic-related risk of T2DM decreased as the patient's baseline body mass index, and baseline fasting plasma glucose level, as well as the dietary proportion of animal products, increased.ConclusionAntipsychotics, especially clozapine, risperidone, chlorpromazine, and perphenazine, increased the T2DM risk among NBPHSP enrollees with schizophrenia. Mental health officers should accurately identify enrollees at a high risk of T2DM and take appropriate preventive measures to reduce the incidence of T2DM among patients with schizophrenia.</jats:sec

A Fast Method for Calculation of Marine Gravity Anomaly

Gravity data have been playing an important role in marine exploration and research. However, obtaining gravity data over an extensive marine area is expensive and inefficient. In reality, marine gravity anomalies are usually calculated from satellite altimetry data. Over the years, numerous methods have been presented for achieving this purpose, most of which are time-consuming due to the integral calculation over a global region and the singularity problem. This paper proposes a fast method for the calculation of marine gravity anomalies. The proposed method introduces a novel scheme to solve the singularity problem and implements the parallel technique based on a graphics processing unit (GPU) for fast calculation. The details for the implementation of the proposed method are described, and it is tested using the geoid height undulation from the Earth Gravitational Model 2008 (EGM2008). The accuracy of the presented method is evaluated by comparing it with marine shipboard gravity data. Its efficiency is demonstrated through comparison with the conventional sequential method. The tests demonstrate that the proposed method can be employed for accurately calculating marine gravity anomalies and provides an advantage on computational efficiency

A Fast Method for Calculation of Marine Gravity Anomaly

Gravity data have been playing an important role in marine exploration and research. However, obtaining gravity data over an extensive marine area is expensive and inefficient. In reality, marine gravity anomalies are usually calculated from satellite altimetry data. Over the years, numerous methods have been presented for achieving this purpose, most of which are time-consuming due to the integral calculation over a global region and the singularity problem. This paper proposes a fast method for the calculation of marine gravity anomalies. The proposed method introduces a novel scheme to solve the singularity problem and implements the parallel technique based on a graphics processing unit (GPU) for fast calculation. The details for the implementation of the proposed method are described, and it is tested using the geoid height undulation from the Earth Gravitational Model 2008 (EGM2008). The accuracy of the presented method is evaluated by comparing it with marine shipboard gravity data. Its efficiency is demonstrated through comparison with the conventional sequential method. The tests demonstrate that the proposed method can be employed for accurately calculating marine gravity anomalies and provides an advantage on computational efficiency

Soil Aggregation and Organic Carbon Dynamics in Poplar Plantations

Soil resident water-stable macroaggregates (diameter (&Oslash;) &gt; 0.25 mm) play a critical role in organic carbon conservation and fertility. However, limited studies have investigated the direct effects of stand development on soil aggregation and its associated mechanisms. Here, we examined the dynamics of soil organic carbon, water-stable macroaggregates, litterfall production, fine-root (&Oslash; &lt; 1 mm) biomass, and soil microbial biomass carbon with stand development in poplar plantations (Populus deltoides L. &lsquo;35&rsquo;) in Eastern Coastal China, using an age sequence (i.e., five, nine, and 16 years since plantation establishment). We found that the quantity of water-stable macroaggregates and organic carbon content in topsoil (0&ndash;10 cm depth) increased significantly with stand age. With increasing stand age, annual aboveground litterfall production did not differ, while fine-root biomass sampled in June, August, and October increased. Further, microbial biomass carbon in the soil increased in June but decreased when sampled in October. Ridge regression analysis revealed that the weighted percentage of small (0.25 mm &le; &Oslash; &lt; 2 mm) increased with soil microbial biomass carbon, while that of large aggregates (&Oslash; &ge; 2 mm) increased with fine-root biomass as well as microbial biomass carbon. Our results reveal that soil microbial biomass carbon plays a critical role in the formation of both small and large aggregates, while fine roots enhance the formation of large aggregates

Phragmites australis + Typha latifolia Community Enhanced the Enrichment of Nitrogen and Phosphorus in the Soil of Qin Lake Wetland

Aquatic plants play an essential role and are effective in mitigating lake eutrophication by forming complex plant-soil system and retaining total nitrogen (TN) and phosphorus (TP) in soils to ultimately reduce their quantities in aquatic systems. Two main vegetation types (Phragmites australis community and P. australis + Typha latifolia community) of Qin Lake wetland were sampled in this study for the analysis of TN and TP contents and reserves in the wetland soils. The results showed that (1) the consumption effect of Qin Lake wetland on soluble N was much more significant than on soluble P. (2) The efficiency of TN enrichment in wetland soil was enhanced by vegetation covering of P. australis and T. latifolia. (3) Wetland soil P was consumed by P. australis community and this pattern was relieved with the introduction of T. latifolia. (4) According to the grey relativity analysis, the most intensive interaction between plants and soil occurred in summer. In addition, the exchange of N in soil-vegetation system primarily occurred in the 0–15 cm soil layer. Our results indicated that vegetation covering was essential to the enrichment of TN and TP, referring to the biology-related fixation in the wetland soil