Consistent splitting of event streams in parallel complex event processing

Complex Event Processing (CEP) combines streams of simple events from multiple sources and infer high-level knowledge by correlating the simple events. The correlation of events is done according to the requirement of the application. This requirement is specified to the CEP system in the form of rules. State of the art implementations of CEP system deploy a distributed network of operators to execute a rule. For low latency information retrieval under high event rate of input streams, scalability of operators is required. This is done by the parallelization of operator functionality. The existing PACE system provides a framework for operator parallelization. Parameter context in a rule specify which events in the input stream can be used for correlation and are essential for application of CEP systems to many real world situations. In this thesis operator parallelization techniques, for adopting the existing PACE system to execute rules with various parameter contexts, are proposed. In cases where the existing PACE system cannot be adopted, other approaches for operator parallelization are proposed. Finally, the proposed techniques are analysed by implementing the techniques and evaluating their performance

AN ARDUINO-BASED WOMEN SAFETY NETWORK MODEL

An embedded product is a mix of hardware and software to carry out a dedicated task. A few of the primary devices utilized in embedded goods are Microprocessors and Microcontrollers. Microprocessors are generally known as general purpose processors because they simply accept the inputs, process it and provide the output. In comparison, a microcontroller not just accepts the information as inputs but additionally manipulates it, interfaces the information with assorted devices, controls the information and therefore finally provides the result. The Gps navigation and GSM based women home security system using Arduino Controller Microcontroller is definitely an exclusive project that may provide to safeguard women based on the instructions provided by the above mentioned stated microcontroller. This project uses controlled 5V, 500ma power. Unregulated 12V Electricity can be used for relay. 7805 three terminal current regulator can be used for current regulation. Bridge type full wave rectifier can be used to rectify the ac creation of secondary of 230/12V step lower transformer

GlycomicsDB - A Data Integration Platform for Glycans and their Structures

Glycomics is a discipline of biology that deals with the structure and function of glycans (or carbohydrates). Analytical techniques such as mass spectrometry (MS) and nuclear magnetic resonance (NMR) are having a significant impact on the field of glycomics. However, effective progress in glycomics research requires collaboration between laboratories to share experimental data, structural information of glycans, and simulation results. Herein we report the development of a web-based data management system that can incorporate large volumes of data from disparate sources and organize them into a uniform format for users to store and access. This system enables participating laboratories to set up a shared data repository which members of interdisciplinary teams can access. The system is able to manage and share raw MS data and structural information of glycans

RNA-binding proteins in eye development and disease: implication of conserved RNA granule components

The molecular biology of metazoan eye development is an area of intense investigation. These efforts have led to the surprising recognition that although insect and vertebrate eyes have dramatically different structures, the orthologs or family members of several conserved transcription and signaling regulators such as Pax6, Six3, Prox1, and Bmp4 are commonly required for their development. In contrast, our understanding of posttranscriptional regulation in eye development and disease, particularly regarding the function of RNA-binding proteins (RBPs), is limited. We examine the present knowledge of RBPs in eye development in the insect model Drosophila as well as several vertebrate models such as fish, frog, chicken, and mouse. Interestingly, of the 42 RBPs that have been investigated for their expression or function in vertebrate eye development, 24 (~60%) are recognized in eukaryotic cells as components of RNA granules such as processing bodies, stress granules, or other specialized ribonucleoprotein (RNP) complexes. We discuss the distinct developmental and cellular events that may necessitate potential RBP/RNA granule-associated RNA regulon models to facilitate posttranscriptional control of gene expression in eye morphogenesis. In support of these hypotheses, three RBPs and RNP/RNA granule components Tdrd7, Caprin2, and Stau2 are linked to ocular developmental defects such as congenital cataract, Peters anomaly, and microphthalmia in human patients or animal models. We conclude by discussing the utility of interdisciplinary approaches such as the bioinformatics tool iSyTE (integrated Systems Tool for Eye gene discovery) to prioritize RBPs for deriving posttranscriptional regulatory networks in eye development and disease. WIREs RNA 2016, 7:527-557. doi: 10.1002/wrna.1355 For further resources related to this article, please visit the WIREs website

Development of Epoxyeicosatrienoic Acid Analogs with in Vivo Anti-Hypertensive Actions

Epoxyeicosatrienoic acids (EETs) contribute importantly to the regulation of vascular tone and blood pressure control. The purpose of this study was to develop stable EET analogs and test their in vivo blood pressure lowering effects in hypertensive rats. Using the pharmacophoric moiety of EETs, ether EET analogs were designed with improved solubility and resistance to auto-oxidation and metabolism by soluble epoxide hydrolase. Ether EET analogs were chosen based on their ability to dilate afferent arterioles and subsequently tested for blood pressure lowering effects in rodent models of hypertension. Initially, 11,12-ether-EET-8-ZE failed to lower blood pressure in angiotensin hypertension or spontaneously hypertensive rats (SHR). Esterification of the carboxylic group of 11,12-ether-EET-8-ZE prevented blood pressure increase in SHR when injected at 2 mg/day for 12 days (MAP Δ change at day 8 of injection was −0.3 ± 2 for treated and 12 ± 1 mmHg for control SHR). Amidation of the carboxylic group with aspartic acid produced another EET analog (NUDSA) with a blood pressure lowering effect when injected at 3 mg/day in SHR for 5 days. Amidation of the carboxylic group with lysine amino acid produced another analog with minimal blood pressure lowering effect. These data suggest that esterification of the carboxylic group of 11,12-ether-EET-8-ZE produced the most effective ether-EET analog in lowering blood pressure in SHR and provide the first evidence to support the use of EET analogs in treatment of cardiovascular diseases

The Tudor-domain protein TDRD7, mutated in congenital cataract, controls the heat shock protein HSPB1 (HSP27) and lens fiber cell morphology

Mutations of the RNA granule component TDRD7 (OMIM: 611258) cause pediatric cataract. We applied an integrated approach to uncover the molecular pathology of cataract in Tdrd7−/− mice. Early postnatal Tdrd7−/− animals precipitously develop cataract suggesting a global-level breakdown/misregulation of key cellular processes. High-throughput RNA sequencing integrated with iSyTE-bioinformatics analysis identified the molecular chaperone and cytoskeletal modulator, HSPB1, among high-priority downregulated candidates in Tdrd7−/− lens. A protein fluorescence two-dimensional difference in-gel electrophoresis (2D-DIGE)-coupled mass spectrometry screen also identified HSPB1 downregulation, offering independent support for its importance to Tdrd7−/− cataractogenesis. Lens fiber cells normally undergo nuclear degradation for transparency, posing a challenge: how is their cell morphology, also critical for transparency, controlled post-nuclear degradation? HSPB1 functions in cytoskeletal maintenance, and its reduction in Tdrd7−/− lens precedes cataract, suggesting cytoskeletal defects may contribute to Tdrd7−/− cataract. In agreement, scanning electron microscopy (SEM) revealed abnormal fiber cell morphology in Tdrd7−/− lenses. Further, abnormal phalloidin and wheat germ agglutinin (WGA) staining of Tdrd7−/− fiber cells, particularly those exhibiting nuclear degradation, reveals distinct regulatory mechanisms control F-actin cytoskeletal and/or membrane maintenance in post-organelle degradation maturation stage fiber cells. Indeed, RNA immunoprecipitation identified Hspb1 mRNA in wild-type lens lysate TDRD7-pulldowns, and single-molecule RNA imaging showed co-localization of TDRD7 protein with cytoplasmic Hspb1 mRNA in differentiating fiber cells, suggesting that TDRD7–ribonucleoprotein complexes may be involved in optimal buildup of key factors. Finally, Hspb1 knockdown in Xenopus causes eye/lens defects. Together, these data uncover TDRD7’s novel upstream role in elevation of stress-responsive chaperones for cytoskeletal maintenance in post-nuclear degradation lens fiber cells, perturbation of which causes early-onset cataracts

Vegetable grafting: a scientific innovation to enhance productivity and profitability of tomato growers under climate change

IntroductionVegetable grafting is a recent innovation in vegetable cultivation that has a great potential for enhancing crop productivity and profitability under climate change scenarios, besides its potential to reduce the cost of cultivation.MethodsThe present strategic research focused on assessing the performance of grafted and non-grafted tomato cultivars (PHS-448 & Sahoo) in Naturally Ventilated Polyhouse (NVPH) and open field (OF) conditions.Results and discussionThe results revealed that grafted tomatoes expressed significantly (p<0.05) higher values of growth parameters, i.e., leaf area and chlorophyll content, contributing to significantly higher total yield over non-grafted tomatoes. The grafted tomato cultivars, viz, PHS-448 and Sahoo, recorded an increased total yield of 36.65% and 46.7% respectively compared to the non-grafted ones. Growing grafted tomatoes under NVPH conditions increased yields by 63.79% due to an increase in pickings (by 3 to 5 times) compared to non-grafted tomatoes grown under open field conditions. The system productivity followed similar yield trends and revealed significant (p<0.05) variation across all picking days. The profitability analysis (gross and net monetary returns and benefit-cost ratio) showed that grafted combinations grown under NVPH are more profitable than the open field conditions and non-grafted ones, grown under both conditions. The multiple regression analysis revealed a strong correlation (R2= >80) of yield with plant height, middle leaves chlorophyll, and leaf area, irrespective of the grafted and non-grafted combinations. The present investigation concluded that cultivating grafted tomatoes helps farmers achieve maximum productivity and profitability in both NVPH and open field conditions. However, a proper policy framework is necessary to promote and scale up grafted vegetable technology to enhance the profitability of vegetable growers in climate change scenarios

A hub and spoke nuclear lamina architecture in trypanosomes

The nuclear lamina supports many functions, including maintaining nuclear structure and gene expression control, and correct spatio-temporal assembly is vital to meet these activities. Recently, multiple lamina systems have been described that, despite independent evolutionary origins, share analogous functions. In trypanosomatids the two known lamina proteins, NUP-1 and NUP-2, have molecular masses of 450 and 170 kDa, respectively, which demands a distinct architecture from the ∼60 kDa lamin-based system of metazoa and other lineages. To uncover organizational principles for the trypanosome lamina we generated NUP-1 deletion mutants to identify domains and their arrangements responsible for oligomerization. We found that both the N- and C-termini act as interaction hubs, and that perturbation of these interactions impacts additional components of the lamina and nuclear envelope. Furthermore, the assembly of NUP-1 terminal domains suggests intrinsic organizational capacity. Remarkably, there is little impact on silencing of telomeric variant surface glycoprotein genes. We suggest that both terminal domains of NUP-1 have roles in assembling the trypanosome lamina and propose a novel architecture based on a hub-and-spoke configuration

Cascade failure analysis of electrical transmission lines using ADINA

Cascade failure of electrical transmission lines can be caused by a number of reasons. Dynamic forces like equipment failure, conductors rupture in any span, heavy wind loads, snow accumulation on conductors and structural members or ice shedding are known to be the main reasons for such a collapse. The primary objective of this investigation was to study the dynamic behavior of supporting towers, collapse of supporting towers and longitudinal dynamic maximum forces that were induced on the adjacent supporting towers due to conductors rupture in any span. The study was performed on transmission lines, whose supporting towers were modeled with (a) Linear elastic truss and beam elements, (b) Moment-Curvature beam elements, with elastic-plastic material properties, and (c) Towers with load limiting devices or tower load controllers (TLC). The transmission lines modeled with linear elastic truss and beam elements were used to perform the free vibration analysis to calculate the damping properties of the towers and the transmission lines. Each of these models was used to conduct static and transient dynamic analyses due to broken conductors in the middle span. The transient dynamic analyses were carried out with bare conductor (no-ice-loads) loading and 25-mm (1-inch) radial ice loading conditions. The linear material models do not predict the failure or cascading of the transmission line towers, however considering the elements as moment-curvature beam models with elasto-plastic materials showed that the towers adjacent to the span, where conductors are ruptured, experienced large impact forces causing the adjacent towers to collapse. And the use of TLCs was able to limit the length of this cascade failure