Innovative Method for the Estimation of Closure Velocity between RAT Driven Drogue and IFR Probe Air to Air refueling Flight Trials

Air-to-air refueling for a fighter platform is a force multiplier in terms of increasing its combat radius and payload carrying ability. Adapting for such a facility especially for an aircraft under design and development is a challenging task. It requires rigorous ground and flight testing to meet the certification standards. One of important flight test parameter that needs to be validated for structural impact load calculations and certification needs is the closure velocity. The air-to-air refueller was equipped with a Ram-air-turbine powered drogue and chute system. An innovative methodology of estimating the closure velocity between the drogue of the mother aircraft and the in-flight refueling probe of the receiving aircraft was evolved. The method was employed and validated during the air-to-air refueling trials of a prototype fighter platform. The intention of this paper is to explain the methodology employed and deliberate the results obtained with respect to the air-to-air refueling certification

Design and development of technologies for decentralized diagnostic testing

Over the past decade, and accelerated due to the COVID-19 pandemic, there has been increasing adoption of decentralized diagnostic testing, where the testing is brought closer to the patient. This trend has largely been fueled by the development of more accurate diagnostic tools and faster and more reliable data connectivity. Decentralized testing has been shown to greatly reduce turnaround times while increasing accessibility to users in remote regions. However, there are challenges that limit its widespread adoption. In this dissertation, we detail the development of tools and technologies to overcome these barriers and expedite the shift towards decentralized diagnostic testing. First, we demonstrate the ability to develop point-of-care (POC) diagnostic tests with performance that rivals that of traditional lab-based methods. We developed a rapid, multiplexed, microfluidic serological test for Lyme disease, a tick-borne disease caused by the Borrelia burgdorferi bacterium. The recommended testing, the standard 2-tiered (STT) approach, is not sensitive for early-stage infections, is labor-intensive, has long turnaround times, and requires the use of two immunoassays (enzyme-linked immunosorbent assay (ELISA) and the Western Blot). We developed a standalone multiplexed sandwich ELISA assay and adapted it to the mChip microfluidic platform. We validated the assay on a rigorously characterized panel of human serum samples and demonstrated that our approach outperforms the STT algorithm on sensitivity while matching its specificity. The form factor of this technology is amenable to use in physician’s offices and urgent care clinics. We also showed exploratory work towards adapting the mChip platform for diagnosis of Zika disease, a mosquito-borne disease caused by the Zika virus, and acute kidney injury, a syndrome characterized by loss of kidney excretory function. Next, we worked on increasing the adoption of rapid diagnostic tests for self- and partner-testing designed to be used in at-home settings. We developed a smartphone application to be used alongside the INSTI Multiplex test for detecting HIV and syphilis infections. The application was designed to provide users with i) instructions on running the test, ii) an automated deep-learning-based image interpretation algorithm to interpret the rapid test results from a smartphone image, iii) a way to save test results and display/share them, and iv) resources for follow-up care. We adopted a user-centered, iterative design process where we worked with a cohort of study participants composed of men who have sex with men and transgender women at high risk for contracting sexually transmitted infections. We then field tested the application with 48 participants over a duration of three months and found high acceptability for the application, both in terms of functionality and helpfulness. Finally, we sought to address a key limitation with deep-learning-based image classification techniques, specifically, the requirement for large numbers of annotated images for training. We developed a deep-learning image interpretation algorithm that could be quickly adapted to new rapid test kits using only a fraction of the images that would otherwise be needed for training the model. The interpretation algorithm followed a three-step, modular process. First, the rapid test kit and the membrane were extracted from the smartphone image. Second, the constituent zones were cropped from the extracted membrane. Finally, a classifier detected the presence or absence of a line in the individual zones. Fast adaptation was demonstrated by adapting a base model, trained using images of a single COVID-19 rapid test kit, to four different rapid test kits, each with different form factors, using few-shot domain adaptation. After training with 20 or fewer images, the classification accuracies of all the adapted models were > 95%. This approach can provide a digital health platform for improved pandemic preparedness and enable quality assurance and linkage to care for consumers operating new LFAs in widespread decentralized settings. Together, these methods provide a suite of tools that could expedite the shift towards decentralized, POC testing

Safety and Efficacy of Bilateral Tubeless Supine Mini-Percutaneous Nephrolithotomy for the Management of Bilateral Renal Calculi in Renal Failure Patients

Background: To evaluate the safety, efficacy, feasibility, stone-free rate, and complications of bilateral tubeless supine mini-percutaneous nephrolithotomy (M-PCNL) for bilateral multiple renal calculi with renal failure as a single-stage procedure. Materials and Methods: We conducted aretrospective study from January 2020 to March 2022 in adult patients with bilateral renal or proximal ureteric calculi with renal failure who were subjected to bilateral supine tubeless M-PCNL. Patients on regular hemodialysis before the procedure were excluded. Data regarding the demographic profile, stone characteristics on non-contrast computed tomography (NCCT), duration of surgery, complications, and auxiliary procedures were retrieved from clinical records. Residual stone fragments of≤4mm in NCCT were considered clinically insignificant. The Clinical Research Office of the Endourological Society validation ofClavien score for PCNL complications was used. Results: A total of twenty-seven patients with a mean age of 45.9years were included in this study. The mean size of stone diameter per renal unit was 2.4 ± 0.4 cm. The mean preoperative serum creatinine was 2.8 mg/dL. A total of 62 tracts and 27 sessions were required for complete treatment of all 54 renal units in the 27 successfully treated patients. The average operating time was 75 (52–122) min on both sides. Serum creatinine drop at onemonth postsurgery was statistically significant (p Conclusion: Bilateral tubeless supine M-PCNL for bilateral renal calculi in selective patients with renal failure in a single session is a safe, feasible, and effective option which can be carried out without increased morbidity and can be attempted if the first-side M-PCNL has gone smoothly within a reasonable amount of time

Point-of-care diagnostics: recent developments in a pandemic age

This article reviews the rapid and unprecedented development, scaleup and deployment of POC devices in response to the COVID-19 pandemic and its implications for the future of diagnostics and digital health.</jats:p

Innovative Method for the Estimation of Closure Velocity between RAT Driven Drogue and IFR Probe Air to Air refueling Flight Trials

Air-to-air refueling for a fighter platform is a force multiplier in terms of increasing its combat radius and payload carrying ability. Adapting for such a facility especially for an aircraft under design and development is a challenging task. It requires rigorous ground and flight testing to meet the certification standards. One of important flight test parameter that needs to be validated for structural impact load calculations and certification needs is the closure velocity. The air-to-air refueller was equipped with a Ram-air-turbine powered drogue and chute system. An innovative methodology of estimating the closure velocity between the drogue of the mother aircraft and the in-flight refueling probe of the receiving aircraft was evolved. The method was employed and validated during the air-to-air refueling trials of a prototype fighter platform. The intention of this paper is to explain the methodology employed and deliberate the results obtained with respect to the air-to-air refueling certification.</jats:p

Integrating user behavior with engineering design of point-of-care diagnostic devices: theoretical framework and empirical findings

As point-of-care diagnostic devices become increasingly available to untrained users, it will be critical to understand how user behavior can best guide the engineering design process.</p