Leaving No One Behind

Crecimiento inclusivo definido por la OCDE como económica crecimiento que se distribuye de manera justa en toda la sociedad y crea Oportunidades para todos. Simplemente puesto, es el crecimiento lo que beneficia todo el mundo. Es cada vez más reconocido internacionalmente que junto con los riesgos sistémicos para el sistema financiero y para la sociedad, desde el cambio climático, es el riesgo sistémico paralelo de hiper global desigualdades Estas son dos caras de la misma moneda: ambas exigir un cambio transformador en los negocios y en la sociedad en La próxima década. Si el crecimiento económico solo lo disfrutan los pocos, no será sostenido por muchos. Igualmente, prosperidad tiene que lograrse dentro de las limitaciones de One Planet. Los líderes empresariales internacionales ya están reconociendo estos desafíos gemelos En abril de 2019, por ejemplo, el Financial Times publicó un artículo de página completa titulado "Por qué los CEOs estadounidenses están preocupados por el futuro del capitalismo ". The Financial Posteriormente, Times ha ejecutado una serie de funciones, OpEds y editoriales sobre el restablecimiento del capitalismo. En agosto de 2019, 180 los principales CEOs corporativos de EE. UU. emitieron un comunicado redefiniendo el Propósito de los negocios. Un año en proceso, esta declaración fue redactado y refinado por los propios CEOs. Allí También ha habido una serie de iniciativas empresariales para "Renovar" o "reimaginar el capitalismo" y promover e inclusión económica. Empresas y liderados por negocios Coaliciones de responsabilidad corporativa en muchas partes del mundo se han unido en estos esfuerzos. Esto incluye Maala: Israel Empresa para la Responsabilidad Social

A unique tRNA recognition mechanism of Caenorhabditis elegans mitochondrial EF-Tu2

Nematode mitochondria expresses two types of extremely truncated tRNAs that are specifically recognized by two distinct elongation factor Tu (EF-Tu) species named EF-Tu1 and EF-Tu2. This is unlike the canonical EF-Tu molecule that participates in the standard protein biosynthesis systems, which basically recognizes all elongator tRNAs. EF-Tu2 specifically recognizes Ser-tRNA(Ser) that lacks a D arm but has a short T arm. Our previous study led us to speculate the lack of the D arm may be essential for the tRNA recognition of EF-Tu2. However, here, we showed that the EF-Tu2 can bind to D arm-bearing Ser-tRNAs, in which the D–T arm interaction was weakened by the mutations. The ethylnitrosourea-modification interference assay showed that EF-Tu2 is unique, in that it interacts with the phosphate groups on the T stem on the side that is opposite to where canonical EF-Tu binds. The hydrolysis protection assay using several EF-Tu2 mutants then strongly suggests that seven C-terminal amino acid residues of EF-Tu2 are essential for its aminoacyl-tRNA-binding activity. Our results indicate that the formation of the nematode mitochondrial (mt) EF-Tu2/GTP/aminoacyl-tRNA ternary complex is probably supported by a unique interaction between the C-terminal extension of EF-Tu2 and the tRNA

Recognition of tRNALeu by Aquifex aeolicus leucyl-tRNA synthetase during the aminoacylation and editing steps

Recognition of tRNA by the cognate aminoacyl-tRNA synthetase during translation is crucial to ensure the correct expression of the genetic code. To understand tRNALeu recognition sets and their evolution, the recognition of tRNALeu by the leucyl-tRNA synthetase (LeuRS) from the primitive hyperthermophilic bacterium Aquifex aeolicus was studied by RNA probing and mutagenesis. The results show that the base A73; the core structure of tRNA formed by the tertiary interactions U8–A14, G18–U55 and G19–C56; and the orientation of the variable arm are critical elements for tRNALeu aminoacylation. Although dispensable for aminoacylation, the anticodon arm carries discrete editing determinants that are required for stabilizing the conformation of the post-transfer editing state and for promoting translocation of the tRNA acceptor arm from the synthetic to the editing site

Features of 80S mammalian ribosome and its subunits

It is generally believed that basic features of ribosomal functions are universally valid, but a systematic test still stands out for higher eukaryotic 80S ribosomes. Here we report: (i) differences in tRNA and mRNA binding capabilities of eukaryotic and bacterial ribosomes and their subunits. Eukaryotic 40S subunits bind mRNA exclusively in the presence of cognate tRNA, whereas bacterial 30S do bind mRNA already in the absence of tRNA. 80S ribosomes bind mRNA efficiently in the absence of tRNA. In contrast, bacterial 70S interact with mRNA more productively in the presence rather than in the absence of tRNA. (ii) States of initiation (Pi), pre-translocation (PRE) and post-translocation (POST) of the ribosome were checked and no significant functional differences to the prokaryotic counterpart were observed including the reciprocal linkage between A and E sites. (iii) Eukaryotic ribosomes bind tetracycline with an affinity 15 times lower than that of bacterial ribosomes (Kd 30 μM and 1–2 μM, respectively). The drug does not effect enzymatic A-site occupation of 80S ribosomes in contrast to non-enzymatic tRNA binding to the A-site. Both observations explain the relative resistance of eukaryotic ribosomes to this antibiotic

RNase T1 mimicking artificial ribonuclease

Recently, artificial ribonucleases (aRNases)—conjugates of oligodeoxyribonucleotides and peptide (LR)4-G-amide—were designed and assessed in terms of the activity and specificity of RNA cleavage. The conjugates were shown to cleave RNA at Pyr-A and G–X sequences. Variations of oligonucleotide length and sequence, peptide and linker structure led to the development of conjugates exhibiting G–X cleavage specificity only. The most efficient catalyst is built of nonadeoxyribonucleotide of unique sequence and peptide (LR)4-G-NH2 connected by the linker of three abasic deoxyribonucleotides (conjugate pep-9). Investigation of the cleavage specificity of conjugate pep-9 showed that the compound is the first single-stranded guanine-specific aRNase, which mimics RNase T1. Rate enhancement of RNA cleavage at G–X linkages catalysed by pep-9 is 108 compared to non-catalysed reaction, pep-9 cleaves these linkages only 105-fold less efficiently than RNase T1 (kcat_RNase T1/kcat_pep-9 = 105)

The 3′-Terminal Hexamer Sequence of Classical swine fever virus RNA Plays a Role in Negatively Regulating the IRES-Mediated Translation

The 3′ untranslated region (UTR) is usually involved in the switch of the translation and replication for a positive-sense RNA virus. To understand the 3′ UTR involved in an internal ribosome entry site (IRES)-mediated translation in Classical swine fever virus (CSFV), we first confirmed the predicted secondary structure (designated as SLI, SLII, SLIII, and SLIV) by enzymatic probing. Using a reporter assay in which the luciferase expression is under the control of CSFV 5′ and 3′ UTRs, we found that the 3′ UTR harbors the positive and negative regulatory elements for translational control. Unlike other stem loops, SLI acts as a repressor for expression of the reporter gene. The negative cis-acting element in SLI is further mapped to the very 3′-end hexamer CGGCCC sequence. Further, the CSFV IRES-mediated translation can be enhanced by the heterologous 3′-ends such as the poly(A) or the 3′ UTR of Hepatitis C virus (HCV). Interestingly, such an enhancement was repressed by flanking this hexamer to the end of poly(A) or HCV 3′ UTR. After sequence comparison and alignment, we have found that this hexamer sequence could hypothetically base pair with the sequence in the IRES IIId1, the 40 S ribosomal subunit binding site for the translational initiation, located at the 5′ UTR. In conclusion, we have found that the 3′-end terminal sequence can play a role in regulating the translation of CSFV

The 3'-end of turnip yellow mosaic virus RNA : application of novel sequencing techniques.

Thesis: Ph. D., Massachusetts Institute of Technology, Department of Biology, 1977Vita.Includes bibliographical references.Ph. D.Ph. D. Massachusetts Institute of Technology, Department of Biolog

In the Eyes of the Beholder: The Complexion of the Shoah in the Lublin District

This article is part of the special cluster titled Conceptualizations of the Holocaust in Germany, Lithuania, Poland, and Ukraine since the 1990s, guest edited by Grzegorz Rossoliński-Liebe. The article addresses sources for understanding the complexion of the Shoah in Poland, through a focus on the Lublin District and Jewish forced labor there. From the opening story of the wedding of Shamai Grajer and Mina Fiszman in Lublin on April 17, 1942, the article extrapolates several central themes: two constants in Nazi policies and Jewish experience—forced population movements and forced labor, the behavior of the various actors involved in the story, and sources. The main individuals involved in the opening story highlight these subjects. Fiszman was a refugee deported in February 1940 from Stettin. Grajer, Fiszman, and Rabbi Zvi Elimelech Talmud, who performed the wedding, had all been selected as forced laborers when the majority of the Jewish community was murdered during the previous month, and they hoped that their labor would help them survive. The behavior of the main German actors in the story, Harry Sturm and Hermann Worthoff, was not uniformly evil, and the behavior of the Jewish actors was not uniformly “heroic.” The Bełżec forced labor complex in 1940 highlights the brutality and murderousness of much of the early forced labor in Poland. Yet, during the deportations to death in 1942 the Jews needed to “unlearn” the lessons of avoiding such labor if they were now to have a hope of surviving. Among the varied sources for this and the subsequent subjects addressed in the article, the Jewish sources provide a sense of what actually happened in these camps and situations. </jats:p

Holocaust survivors: experiences of displacement and narratives of self

Looking at Displaced People (predominantly Jewish) after the Second World War, the dissertation begins by problematising and broadening the term ‘displacement’, from its UNRRA definition (primarily geopolitical) to embrace profound existential concerns. It then explores potential trends in the ways in which DPs reacted to new challenges posed by the ‘initiatory crisis’ of liberation. Considering factors such as age, role, rupture, emigration, the changing role of global perceptions of the ‘Holocaust survivor’ and the formal constraints and idiomatic influences at work in ordering and recording memory for consumption by projected audiences, it argues for the validity of memoirs and testimonies as a primary source-base, revelatory of patterns in behaviour and belief systems that an analysis based on outward behaviour alone might overlook. Through close attention to the life stories of survivors, situated amid contextualising detail, it will be seen that the DP population emerging from the Holocaust constitutes a unique and historically fascinating group in terms of representing a collective and sustained form of grappling with the nature of experience, memory, community, and value. The study develops and suggests extrapolation of a new vocabulary of terms dealing with processes of narrativisation which may be of wider applicability