Prevalence of inclusion body disease and associated comorbidity in captive collections of boid and pythonid snakes in Belgium

Inclusion body disease (IBD) is caused by reptarenaviruses and constitutes one of the most notorious viral diseases in snakes. Although central nervous system disease and various other clinical signs have been attributed to IBD in boid and pythonid snakes, studies that unambiguously reveal the clinical course of natural IBD and reptarenavirus infection are scarce. In the present study, the prevalence of IBD and reptarenaviruses in captive snake collections and the correlation of IBD and reptarenavirus infection with the clinical status of the sampled snakes were investigated. In three IBD positive collections, long-term follow-up during a three- to seven-year period was performed. A total of 292 snakes (178 boas and 114 pythons) from 40 collections in Belgium were sampled. In each snake, blood and buffy coat smears were evaluated for the presence of IBD inclusion bodies (IB) and whole blood was tested for reptarenavirus RNA by RT-PCR. Of all tested snakes, 16.5% (48/292) were positive for IBD of which all were boa constrictors (34.0%; 48/141) and 17.1% (50/292) were reptarenavirus RT-PCR positive. The presence of IB could not be demonstrated in any of the tested pythons, while 5.3% (6/114) were reptarenavirus positive. In contrast to pythons, the presence of IB in peripheral blood cells in boa constrictors is strongly correlated with reptarenavirus detection by RT-PCR (P<0.0001). Although boa constrictors often show persistent subclinical infection, long-term follow-up indicated that a considerable number (22.2%; 6/27) of IBD/reptarenavirus positive boas eventually develop IBD associated comorbidities

The infrared imaging spectrograph (IRIS) for TMT: sensitivities and simulations

We present sensitivity estimates for point and resolved astronomical sources for the current design of the InfraRed Imaging Spectrograph (IRIS) on the future Thirty Meter Telescope (TMT). IRIS, with TMT's adaptive optics system, will achieve unprecedented point source sensitivities in the near-infrared (0.84 - 2.45 {\mu}m) when compared to systems on current 8-10m ground based telescopes. The IRIS imager, in 5 hours of total integration, will be able to perform a few percent photometry on 26 - 29 magnitude (AB) point sources in the near-infrared broadband filters (Z, Y, J, H, K). The integral field spectrograph, with a range of scales and filters, will achieve good signal-to-noise on 22 - 26 magnitude (AB) point sources with a spectral resolution of R=4,000 in 5 hours of total integration time. We also present simulated 3D IRIS data of resolved high-redshift star forming galaxies (1 < z < 5), illustrating the extraordinary potential of this instrument to probe the dynamics, assembly, and chemical abundances of galaxies in the early universe. With its finest spatial scales, IRIS will be able to study luminous, massive, high-redshift star forming galaxies (star formation rates ~ 10 - 100 M yr-1) at ~100 pc resolution. Utilizing the coarsest spatial scales, IRIS will be able to observe fainter, less massive high-redshift galaxies, with integrated star formation rates less than 1 M yr-1, yielding a factor of 3 to 10 gain in sensitivity compared to current integral field spectrographs. The combination of both fine and coarse spatial scales with the diffraction-limit of the TMT will significantly advance our understanding of early galaxy formation processes and their subsequent evolution into presentday galaxies.Comment: SPIE Astronomical Instrumentation 201

The Infrared Imaging Spectrograph (IRIS) for TMT: Volume phase holographic grating performance testing and discussion

Maximizing the grating efficiency is a key goal for the first light instrument IRIS (Infrared Imaging Spectrograph) currently being designed to sample the diffraction limit of the TMT (Thirty Meter Telescope). Volume Phase Holographic (VPH) gratings have been shown to offer extremely high efficiencies that approach 100% for high line frequencies (i.e., 600 to 6000l/mm), which has been applicable for astronomical optical spectrographs. However, VPH gratings have been less exploited in the near-infrared, particularly for gratings that have lower line frequencies. Given their potential to offer high throughputs and low scattered light, VPH gratings are being explored for IRIS as a potential dispersing element in the spectrograph. Our team has procured near-infrared gratings from two separate vendors. We have two gratings with the specifications needed for IRIS current design: 1.51-1.82{\mu}m (H-band) to produce a spectral resolution of 4000 and 1.19- 1.37 {\mu}m (J-band) to produce a spectral resolution of 8000. The center wavelengths for each grating are 1.629{\mu}m and 1.27{\mu}m, and the groove densities are 177l/mm and 440l/mm for H-band R=4000 and J-band R=8000, respectively. We directly measure the efficiencies in the lab and find that the peak efficiencies of these two types of gratings are quite good with a peak efficiency of ~88% at the Bragg angle in both TM and TE modes at H-band, and 90.23% in TM mode, 79.91% in TE mode at J-band for the best vendor. We determine the drop in efficiency off the Bragg angle, with a 20-23% decrease in efficiency at H-band when 2.5 degree deviation from the Bragg angle, and 25%-28% decrease at J-band when 5{\deg} deviation from the Bragg angle.Comment: Proceedings of the SPIE, 9147-33

The Infrared Imaging Spectrograph (IRIS) for TMT: the atmospheric dispersion corrector

We present a conceptual design for the atmospheric dispersion corrector (ADC) for TMT's Infrared Imaging Spectrograph (IRIS). The severe requirements of this ADC are reviewed, as are limitations to observing caused by uncorrectable atmospheric effects. The requirement of residual dispersion less than 1 milliarcsecond can be met with certain glass combinations. The design decisions are discussed and the performance of the design ADC is described. Alternative options and their performance tradeoffs are also presented.Comment: SPIE Astronomical Instrumentation 201

The infrared imaging spectrograph (IRIS) for TMT: spectrograph design

The Infra-Red Imaging Spectrograph (IRIS) is one of the three first light instruments for the Thirty Meter Telescope (TMT) and is the only one to directly sample the diffraction limit. The instrument consists of a parallel imager and off-axis Integral Field Spectrograph (IFS) for optimum use of the near infrared (0.84um-2.4um) Adaptive Optics corrected focal surface. We present an overview of the IRIS spectrograph that is designed to probe a range of scientific targets from the dynamics and morphology of high-z galaxies to studying the atmospheres and surfaces of solar system objects, the latter requiring a narrow field and high Strehl performance. The IRIS spectrograph is a hybrid system consisting of two state of the art IFS technologies providing four plate scales (4mas, 9mas, 25mas, 50mas spaxel sizes). We present the design of the unique hybrid system that combines the power of a lenslet spectrograph and image slicer spectrograph in a configuration where major hardware is shared. The result is a powerful yet economical solution to what would otherwise require two separate 30m-class instruments.Comment: 15 pages, 11 figure

Magnetic Resonance Imaging Pilot Study of Intravenous Glyburide in Traumatic Brain Injury.

Pre-clinical studies of traumatic brain injury (TBI) show that glyburide reduces edema and hemorrhagic progression of contusions. We conducted a small Phase II, three-institution, randomized placebo-controlled trial of subjects with TBI to assess the safety and efficacy of intravenous (IV) glyburide. Twenty-eight subjects were randomized and underwent a 72-h infusion of IV glyburide or placebo, beginning within 10 h of trauma. Of the 28 subjects, 25 had Glasgow Coma Scale (GCS) scores of 6-10, and 14 had contusions. There were no differences in adverse events (AEs) or severe adverse events (ASEs) between groups. The magnetic resonance imaging (MRI) percent change at 72-168 h from screening/baseline was compared between the glyburide and placebo groups. Analysis of contusions (7 per group) showed that lesion volumes (hemorrhage plus edema) increased 1036% with placebo versus 136% with glyburide (p = 0.15), and that hemorrhage volumes increased 11.6% with placebo but decreased 29.6% with glyburide (p = 0.62). Three diffusion MRI measures of edema were quantified: mean diffusivity (MD), free water (FW), and tissue MD (MDt), corresponding to overall, extracellular, and intracellular water, respectively. The percent change with time for each measure was compared in lesions (n = 14) versus uninjured white matter (n = 24) in subjects receiving placebo (n = 20) or glyburide (n = 18). For placebo, the percent change in lesions for all three measures was significantly different compared with uninjured white matter (analysis of variance [ANOVA], p &lt; 0.02), consistent with worsening of edema in untreated contusions. In contrast, for glyburide, the percent change in lesions for all three measures was not significantly different compared with uninjured white matter. Further study of IV glyburide in contusion TBI is warranted

A Hubble Space Telescope Snapshot Survey of Dynamically Close Galaxy Pairs in the CNOC2 Redshift Survey

We compare the structural properties of two classes of galaxies at intermediate redshift: those in dynamically close galaxy pairs, and those which are isolated. Both samples are selected from the CNOC2 Redshift Survey, and have redshifts in the range 0.1 < z <0.6. Hubble Space Telescope WFPC2 images were acquired as part of a snapshot survey, and were used to measure bulge fraction and asymmetry for these galaxies. We find that paired and isolated galaxies have identical distributions of bulge fractions. Conversely, we find that paired galaxies are much more likely to be asymmetric (R_T+R_A >= 0.13) than isolated galaxies. Assuming that half of these pairs are unlikely to be close enough to merge, we estimate that 40% +/- 11% of merging galaxies are asymmetric, compared with 9% +/- 3% of isolated galaxies. The difference is even more striking for strongly asymmetric (R_T+R_A >= 0.16) galaxies: 25% +/- 8% for merging galaxies versus 1% +/- 1% for isolated galaxies. We find that strongly asymmetric paired galaxies are very blue, with rest-frame B-R colors close to 0.80, compared with a mean (B-R)_0 of 1.24 for all paired galaxies. In addition, asymmetric galaxies in pairs have strong [OII]3727 emission lines. We conclude that close to half of the galaxy pairs in our sample are in the process of merging, and that most of these mergers are accompanied by triggered star formation.Comment: Accepted for publication in the Astronomical Journal. 40 pages, including 15 figures. For full resolution version, please see http://www.trentu.ca/physics/dpatton/hstpairs

Height and Biomass of Mangroves in Africa from ICEsat/GLAS and SRTM

The accurate quantification of forest 3-D structure is of great importance for studies of the global carbon cycle and biodiversity. These studies are especially relevant in Africa, where deforestation rates are high and the lack of background data is great. Mangrove forests are ecologically significant and it is important to measure mangrove canopy heights and biomass. The objectives of this study are to estimate: 1. The total area, 2. Canopy height distributions and 3. Aboveground biomass of mangrove forests in Africa. To derive mangrove 3-D structure and biomass maps, we used a combination of mangrove maps derived from Landsat ETM+, LiDAR canopy height estimates from ICEsat/GLAS (Ice, Cloud, and land Elevation Satellite/Geoscience Laser Altimeter System) and elevation data from SRTM (Shuttle Radar Topography Mission) for the African continent. More specifically, we extracted mangrove forest areas on the SRTM DEM using Landsat based landcover maps. The LiDAR (Light Detection and Ranging) measurements from the large footprint GLAS sensor were used to derive local estimates of canopy height and calibrate the Interferometric Synthetic Aperture Radar (InSAR) data from SRTM. We then applied allometric equations relating canopy height to biomass in order to estimate above ground biomass (AGB) from the canopy height product. The total mangrove area of Africa was estimated to be 25 960 square kilometers with 83% accuracy. The largest mangrove areas and greatest total biomass was 29 found in Nigeria covering 8 573 km2 with 132 x10(exp 6) Mg AGB. Canopy height across Africa was estimated with an overall root mean square error of 3.55 m. This error also includes the impact of using sensors with different resolutions and geolocation error which make comparison between measurements sensitive to canopy heterogeneities. This study provides the first systematic estimates of mangrove area, height and biomass in Africa. Our results showed that the combination of ICEsat/GLAS and SRTM data is well suited for vegetation 3-D mapping on a continental scale