Quantification of Kaempferol Conjugates in Watercress Juice and Methanol Extract: A Study Using HPLC and Protein Binding

Flavonoids are a large group of biologically active polyphenolic compounds found in plants, that have gained importance in recent years due to their potential medicinal and therapeutic properties of high potency and low systemic toxicity. Kaempferol (3,4′,5,7- tetrahydroxyflavone) is a polyphenol antioxidant dietary flavonoid found in a variety of fruits and leafy vegetables, specifically vegetables of the Brassica family, which includes our subject vegetable, watercress (WC) which is usually grown in marshy lands. Kaempferol (KMP) has been depicted to have revolutionary attribute in overall human health ranging from anti-cancerous to anti-inflammatory properties. Here we show a novel approach for quantifying kaempferol and its derivatives in watercress juice (WC2) and methanol extract (WCM) using HPLC and protein binding studies. Human serum albumin (HSA) is chosen as the model protein

Referral Pathways and Service Connections Among Heirs’ Property Owners in South Carolina

Researchers, practitioners, and policymakers interested in alleviating heirs’ property ownership precarity have long sought to connect these owners to titling and land management resources, but there is limited scholarly evidence on successful interventions. Using administrative data from the Center for Heirs’ Property Preservation®(CHPP®), this article explores the demographic characteristics, types of direct legal services received, and referral pathways of landowners seeking legal assistance from CHPP® between 2017 and 2021. We find that applicants are primarily elderly, Black women, referred through four main pathways: (1) owners’ personal networks, (2) CHPP® outreach efforts, (3) CHPP® partner organizations—including public, private, and nonprofit agencies, and (4) word of mouth (other individuals/entities not formally connected with CHPP®, including outside legal and forestry professionals). Lastly, we identify a strong desire for estate planning amongst applicants, despite documented legal distrust amongst heirs’ property owners. This analysis has important implications for designing targeted interventions to assist heirs’ property owners beyond the South Carolina context

Desmanthus for silage

Introduction: Desmanthus is known as a pasture legume where its inclusion can increase animal performance. However, under a cropping scenario desmanthus can produce high yield of good quality forage (Mwangi et al. 2022). This study was initiated to determine if desmanthus could be preserved as silage. Materials and Methods: Progardes desmanthus, cultivars JCU 4, JCU 6 and JCU 9, were established in three irrigated 4 ha blocks in north Queensland (19°35’S 146°54’E) on 21/12/2021. The blocks were slashed and regrowth was mowed after 60 d on 12/4/22. Within 4 hours of mowing, cultivars were round baled and wrapped in 5 to 8 layers of white plastic film wrap. Bales were stored on their ends outside. On 14/9/22 two bales of each cultivar were unwrapped and presented to a group of 15 beef cows for 24 h for monitoring of feeding behaviour. Samples at ensiling and feed-out were analysed by NIR. Results: The dry matter (DM) at mowing was similar, but JCU 9 silage was drier than the other silages (Table 1) leading to more extensive moulding. The loss of water soluble carbohydrate (WSC) in all silages was matched by a reduction in pH and production of fermentation acids. All silages had a restricted heterotactic fermentation typical of round bale silage. Video monitoring revealed that cattle spent more time at cultivars JCU 4 and 6, possibly due to visibly less mounding in these bales. Conclusions: This preliminary study demonstrated that desmanthus can be ensiled. All three silages were of good nutritive value and satisfactory fermentation and should support good levels of animal production. The higher apparent presence of moulds in the drier JCU 9 silages suggests ensiling above ~ 50% DM increases moulding and reduces preference for the silage

Redefining manufacturing quality control in the electronics industry

Thesis (S.M.)--Massachusetts Institute of Technology, Sloan School of Management; and, (S.M.)--Massachusetts Institute of Technology, Dept. of Materials Science and Engineering; in conjunction with the Leaders for Manufacturing Program, Massachusetts Institute of Technology, 2000.Also available online at the MIT Theses Online homepage .Includes bibliographical references (p. 103).The most time consuming and capital intensive portion in the assembly of power electronic devices is the test system. A comprehensive test system including functional and stress screening technologies can significantly increase assembly times and more than double the capital investment required in a new assembly line. The primary purpose of the test system is to screen components for early life failures and to verify proper assembly. Determination of key performance characteristics and the resultant test system are developed during the product design phase and are seldom revised after the product has been released to manufacturing. This thesis explores best practices in testing methods and develops new methods to analyze test system performance. Both efforts were conducted in an effort to optimize existing test regimes. Upon completion of the above analyses the existing test sequence was reduced by 50%. This was primarily due to a discovery in the Burn In test cycle which indicated that failures correlated strongly with the on/off cycles inherent in the test sequence. A new test cycle was proposed to accommodate this finding and test results verified the initial hypothesis. Additionally, the summary of best practices identified new forms of product testing including Highly Accelerated Stress Testing (HAST), moving additional product testing into the development phase consequently reducing testing requirements during assembly.by Maureen Fresquez Simington.S.M

Influence of Different Levels of Lipoic Acid Synthase Gene Expression on Diabetic Nephropathy

Oxidative stress is implicated in the pathogenesis of diabetic nephropathy (DN) but outcomes of many clinical trials are controversial. To define the role of antioxidants in kidney protection during the development of diabetic nephropathy, we have generated a novel genetic antioxidant mouse model with over- or under-expression of lipoic acid synthase gene (Lias). These models have been mated with Ins2Akita/+ mice, a type I diabetic mouse model. We compare the major pathologic changes and oxidative stress status in two new strains of the mice with controls. Our results show that Ins2Akita/+ mice with under-expressed Lias gene, exhibit higher oxidative stress and more severe DN features (albuminuria, glomerular basement membrane thickening and mesangial matrix expansion). In contrast, Ins2Akita/+ mice with highly-expressed Lias gene display lower oxidative stress and less DN pathologic changes. Our study demonstrates that strengthening endogenous antioxidant capacity could be an effective strategy for prevention and treatment of DN

The use of brief family counseling with middle school students experiencing discipline problems

The purpose of this study was to investigate the use of brief family counseling with middle school students experiencing discipline problems. Participants were 29 middle school students and their families. The study consisted of two experimental treatment groups and a control group. The Behavior Rating Profile-2 was administered pre-and post-treatment. The results are summarized below: 1 . Brief family counseling participants had significantly fewer suspensions than the brief individual and comparison treatment groups. 2. Brief family counseling participants had significantly fewer discipline referrrals than the brief individual and comparison treatment groups. 3. No differences were found between the pre-and post-treatment behavior ratings of students by parents in the brief family counseling group. 4. The control group received higher post-treatment behavior ratings by teachers than the brief family and individual counseling treatment groups. 5. No differences were found between the brief individual and family counseling groups on ratings of behavior by student participants

Characterization of essential eggshell proteins from Aedes aegypti mosquitoes

Background: Up to 40% of the world population live in areas where mosquitoes capable of transmitting the dengue virus, including Aedes aegypti, coexist with humans. Understanding how mosquito egg development and oviposition are regulated at the molecular level may provide new insights into novel mosquito control strategies. Previously, we identified a protein named eggshell organizing factor 1 (EOF1) that when knocked down with RNA interference (RNAi) resulted in non-melanized and fragile eggs that did not contain viable embryos. Results: In this current study, we performed a comprehensive RNAi screen of putative A. aegypti eggshell proteins to identify additional proteins that interact with intracellular EOF1. We identified several proteins essential for eggshell formation in A. aegypti and characterized their phenotypes through a combination of molecular and biochemical approaches. We found that Nasrat, Closca, and Polehole structural proteins, together with the Nudel serine protease, are indispensable for eggshell melanization and egg viability. While all four proteins are predominantly expressed in ovaries of adult females, Nudel messenger RNA (mRNA) expression is highly upregulated in response to blood feeding. Furthermore, we identified four additional secreted eggshell enzymes that regulated mosquito eggshell formation and melanization. These enzymes included three dopachrome-converting enzymes (DCEs) and one cysteine protease. All eight of these eggshell proteins were essential for proper eggshell formation. Interestingly, their eggshell surface topologies in response to RNAi did not phenocopy the effect of RNAi-EOF1, suggesting that additional mechanisms may influence how EOF1 regulates eggshell formation and melanization. Conclusions: While our studies did not identify a definitive regulator of EOF1, we did identify eight additional proteins involved in mosquito eggshell formation that may be leveraged for future control strategies

Mid- to Long-Term Outcomes in Patients After Hip Arthroscopy With Labral Reconstruction: A Systematic Review

BACKGROUND: There is a paucity of aggregate data documenting mid- to long-term outcomes of patients after hip arthroscopy with labral reconstruction. PURPOSE: To report mid- to long-term outcomes in patients after undergoing either primary or revision hip arthroscopy with labral reconstruction for the treatment of irreparable labral tears. STUDY DESIGN: Systematic review; Level of evidence, 4. METHODS: A systematic review of the PubMed, Cochrane, and Scopus databases in May 2022 was conducted with the following keywords: hip arthroscopy, labral reconstruction, irreparable, labrum, reconstruction, five-year, midterm, 5 year, long-term, 10 year, ten-year, and femoroacetabular impingement using the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses criteria. Midterm was defined as mean 5-year follow-up, and long-term was defined as mean 10-year or longer follow-up. For each included article, the demographic, radiographic, intraoperative, and surgical variables, as well as patient-reported outcomes (PROs), psychometric thresholds, and secondary surgeries were recorded. Forest plots were created for PROs that were reported in ≥3 studies; heterogeneity was assessed using RESULTS: Out of 463 initial articles, 5 studies including 178 hips with primary and 41 hips with revision surgeries were included. One study had an average 5-year follow-up, three studies had a minimum 5-year follow-up and one study had a minimum 10-year follow-up. The most common indications for hip arthroscopy with labral reconstruction were irreparable labral tears. The most common PRO was the modified Harris Hip Score (mHHS), which was reported in all 5 studies. The mean preoperative mHHS ranged from 58.9 to 66, and the mean postoperative mHHS at minimum 5-year follow-up ranged from 80.2 to 89. The preoperative and postoperative mHHSs for the single long-term follow-up study were 60 and 82, respectively. All 5 studies demonstrated significant improvements in reported PROs. All 5 studies reported secondary surgery rates, with 1 study reporting rates at both 5- and 10-year follow-up. Conversion to total hip arthroplasty ranged from 0% to 27%, while overall secondary surgery rates ranged from 0% to 36%. CONCLUSION: Findings demonstrated that patients undergoing primary and revision hip arthroscopy with labral reconstruction experienced favorable outcomes and high rates of clinical benefit and survivorship at mid- to long-term follow-up

α-Lipoic acid protects mitochondrial enzymes and attenuates lipopolysaccharide-induced hypothermia in mice

Hypothermia is a key symptom of sepsis, but the mechanism(s) leading to hypothermia during sepsis is largely unknown and thus no effective therapy is available for hypothermia. Therefore, it is important to investigate the mechanisms and develop effective therapeutic methods. Lipopolysaccharide (LPS)-induced hypothermia accompanied by excess nitric oxide (NO) production, lead to a reduction in energy production in wild type mice. However, mice lacking inducible nitric oxide synthase did not suffer from LPS-induced hypothermia, suggesting that hypothermia is associated with excess NO production during sepsis. This observation is supported by the treatment of wild type mice with α-lipoic acid (LA) in that it effectively attenuates LPS-induced hypothermia with decreased NO production. We also found that LA partially restored ATP production, and activities of the mitochondrial enzymes involved in energy metabolism, which were inhibited during sepsis. These data suggest that hypothermia is related to mitochondrial dysfunction, which is likely compromised by excess NO production and that LA administration attenuates hypothermia mainly by protecting mitochondrial enzymes from NO damage