P450 aromatase alterations and DNA damage as avian pollution biomarkers in cliff and cave swallow breeding near the Rio Grande region, Texas

The endocrine system, specifically relating to sex hormones, and genetic material can be targets of environmental contaminants. Environmental contaminants in the Rio Grande region may originate from industrial or agricultural processes and growing populations lacking proper water and sewage infrastructure. Cliff (Petrochelidon pyrrhonota) and cave (P. fulva) swallows breeding near the Rio Grande were selected to monitor aromatase activity alterations and DNA damage. Swallows were sampled at six sites along the Rio Grande from Brownsville to Laredo, and a reference site (Somerville) 350 miles north of the Rio Grande. DNA damage, based on nuclear DNA content, was determined by flow cytometry. A significantly larger mean half peak coefficient of variation (HPCV) of DNA content in contaminated sites compared to a reference site reflects possible chromosomal damage. No detectable HPCV differences were observed in cave swallows among locations, notwithstanding the presence of mutagenic contaminants. Selenium may provide a protective role against genetic damage. However, cliff swallows from Laredo had significantly higher HPCV values than those from Somerville. DNA damage could be attributed to metals and polycyclic aromatic hydrocarbons released near Laredo. Brains and gonads, two estrogen-dependent organs, were tested for aromatase activity with a tritiated water method. Brain aromatase activity was higher, though not always statistically, for male cave and male and female cliff swallows. Dichlorodiphenyldichloroethylene (DDE) may play a role in the increased activity. Female cave swallows in Llano Grande appeared to have a greatly depressed brain aromatase activity, possibly attributed to past human use of toxaphene. Testicular and ovarian aromatase activity in cliff and cave swallows from Rio Grande was higher than in those from Somerville, though not always significantly. DDE, atrazine, sewage treatment plant contaminants (phthalates, alkylphenols, ethynylestradiol), metals, or other pollutants could play a role in the increased gonadal activity. Increased aromatase activity, in association with contaminants, may be easier to detect in testes of male birds which normally exhibit low levels of estrogen. Siterelated contaminants may be playing a role in DNA damage and aromatase alterations. This is the first known study which uses aromatase activity as an endocrine disruptor indicator in wild birds

DER Certification Laboratory Pilot, Accreditation Plan, and Interconnection Agreement Handbook

This report describes the first steps toward creating the organization, procedures, plans and tools for distributed energy resources (DER) equipment certification, test laboratory accreditation, and interconnection agreements. It covers the activities and accomplishments during the first period of a multiyear effort. It summarizes steps taken to outline a certification plan to assist in the future development of an interim plan for certification and accreditation activities. It also summarizes work toward a draft plan for certification, a beta Web site to support communications and materials, and preliminary draft certification criteria

Fast Response, Load-Matching Hybrid Fuel Cell: Final Technical Progress Report

Hybrid DER technologies interconnected with the grid can provide improved performance capabilities compared to a single power source, and, add value, when matched to appropriate applications. For example, in a typical residence, the interconnected hybrid system could provide power during a utility outage, and also could compensate for voltage sags in the utility service. Such a hybrid system would then function as a premium power provider and eliminate the potential need for an uninterruptible power supply. In this research project, a proton exchange membrane (PEM) fuel cell is combined with an asymmetrical ultracapacitor to provide robust power response to changes in system loading. This project also considers the potential of hybrid DER technologies to improve overall power system compatibility and performance. This report includes base year accomplishments of a proposed 3-year-option project

Hospital and Pharmacy Departmental Policies and Procedures for Gene Therapy at a Teaching Institution

Gene therapy is an emerging, promising field in health care. Although still in investigational trials, gene therapy offers utility in a variety of disease states. This article details hospital and departmental policies and procedures for gene therapy research at a teaching institution. These preliminary guidelines identify the standards of practice at one institution in 1999 and will be refined as systems of practice develop. The guidelines, which exemplify the types of precautions necessary for safely utilizing gene therapy, may be useful to other institutions in the process of developing their own policies. </jats:p