Korekcija za nagib osovine alhidade teodolita kod mjerenja deformacija brana

Oprava ze sklonu osy alhidády při mĕřeni deformaci přehrad.Korekcija za nagib osovine alhidade teodolita kod mjerenja deformacija brana

Targeting of U4/U6 small nuclear RNP assembly factor SART3/p110 to Cajal bodies

The spliceosomal small nuclear RNAs (snRNAs) are distributed throughout the nucleoplasm and concentrated in nuclear inclusions termed Cajal bodies (CBs). A role for CBs in the metabolism of snRNPs has been proposed but is not well understood. The SART3/p110 protein interacts transiently with the U6 and U4/U6 snRNPs and promotes the reassembly of U4/U6 snRNPs after splicing in vitro. Here we report that SART3/p110 is enriched in CBs but not in gems or residual CBs lacking coilin. The U6 snRNP Sm-like (LSm) proteins, also involved in U4/U6 snRNP assembly, were localized to CBs as well. The levels of SART3/p110 and LSm proteins in CBs were reduced upon treatment with the transcription inhibitor α-amanitin, suggesting that CB localization reflects active processes dependent on transcription/splicing. The NH2-terminal HAT domain of SART3/p110 was necessary and sufficient for specific protein targeting to CBs. Overexpression of truncation mutants containing the HAT domain had dominant negative effects on U6 snRNP localization to CBs, indicating that endogenous SART3/p110 plays a role in targeting the U6 snRNP to CBs. We propose that U4 and U6 snRNPs accumulate in CBs for the purpose of assembly into U4/U6 snRNPs by SART3/p110

Existence of positive solutions for singular higher-order fractional differential equations with infinite-point boundary conditions

In this paper, we consider a class of singular fractional differential equations with infinite-point boundary conditions. The fractional orders are involved in the nonlinearity of the boundary value problem, and the nonlinearity is allowed to be singular with respect to not only the time variable but also to the space variable. Firstly, we give Green’s function and establish its properties. Then, we utilize the sequential technique and regularization to investigate the existence of positive solutions. Finally, we give an example of application of our result

Mitotic noncoding RNA processing promotes kinetochore and spindle assembly in Xenopus

Transcription at the centromere of chromosomes plays an important role in kinetochore assembly in many eukaryotes, and noncoding RNAs contribute to activation of the mitotic kinase Aurora B. However, little is known about how mitotic RNA processing contributes to spindle assembly. We found that inhibition of transcription initiation or RNA splicing, but not translation, leads to spindle defects in Xenopus egg extracts. Spliceosome inhibition resulted in the accumulation of high molecular weight centromeric transcripts, concomitant with decreased recruitment of the centromere and kinetochore proteins CENP-A, CENP-C, and NDC80 to mitotic chromosomes. In addition, blocking transcript synthesis or processing during mitosis caused accumulation of MCAK, a microtubule depolymerase, on the spindle, indicating misregulation of Aurora B. These findings suggest that co-transcriptional recruitment of the RNA processing machinery to nascent mitotic transcripts is an important step in kinetochore and spindle assembly and challenge the idea that RNA processing is globally repressed during mitosis

A new approach to Boundary value problems on the half line using weakly-strongly sequentially continuous maps

An existence principle is established for a boundary value problem on the half line using a new theory based on weakly-strongly sequentially continuous maps