The role of the dyke in recreational activities along the Wadden Sea area in vicinity of Delfzijl (Groningen).

This research addresses the question on how Delfzijl can take advantage of the dykes referred to recreational activities within the next five years. In order to investigate this problem statement, the elaboration was started with the definition of research questions and secondary research. Here, brochures, websites, books and other researches were used to gather significant information about Delfzijl. Next, quantitative and qualitative research methods were used in order to collect data and information about the different stakeholders, the current situation of the municipality and the expectations of the people concerning the future. Thus, a fieldtrip was done to interview stakeholders and to distribute questionnaires to the sample. Additionally, a benchmarking was done with the German city `Nessmersiel` in order to compare and discover development possibilities. The research outcome is that Delfzijl suffers from a decreasing population number, a low level of recreational attractiveness and lack of money. Many people claim that the dyke is a significant resource that should be used for recreational developments. Hereby, developments can be done on a small budget

Synthetic enzymes for synthetic substrates

In recent years, hydrolases like cutinases, esterases and lipases have been recognized as powerful tools for hydrolysis of synthetic polymers such as polyethylene terephthalate (PET) as an environmentally friendly alternative for environmentally harmful chemical recycling methods1. PET is currently the most common type of aromatic polyester, with widespread application as packaging material, beverage bottles, and synthetic textile fibers. So far, cutinases have been the most active enzyme class regarding PET degradation. In nature, cutinases catalyze the hydrolysis of the aliphatic biopolyester cutin, the structural component of plant cuticle. Although cutinases are able to act on natural insoluble polyesters, their activities on non-natural substrates are quit low. For this reason, different engineering strategies were established to optimize “polyesterases” for synthetic polymers (Fig.1). Thereby, development of rationale enzyme-engineering strategies led to remarkable enhancement of hydrolytic activities on polyesters and clearly showed that the affinity between the enzyme and the substrate plays a key role in the enzymatic hydrolysis of synthetic polyester. Please click Additional Files below to see the full abstract

How to find Mr. McDreamy? a critical discourse analysis of postfeminist romantic fiction

vorgelegt von Birgit SteinkellnerAbweichender Titel laut cbersetzung der Verfasserin/des VerfassersZsfassung in dt. SpracheGraz, Univ., Dipl.-Arb., 200

Plant-based Heterologous Expression of Mal d 2, a Thaumatin-like Protein and Allergen of Apple (Malus domestica), and its Characterization as an Antifungal Protein

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The sustainable synthesis of levetiracetam by an enzymatic dynamic kinetic resolution and an <i>ex-cell</i> anodic oxidation

Levetiracetam is an active pharmaceutical ingredient widely used to treat epilepsy.</p

In vivo amphetamine action is contingent on αCaMKII

Addiction to psychostimulants (ie, amphetamines and cocaine) imposes a major socioeconomic burden. Prevention and treatment represent unmet medical needs, which may be addressed, if the mechanisms underlying psychostimulant action are understood. Cocaine acts as a blocker at the transporters for dopamine (DAT), serotonin (SERT), and norepinephrine (NET), but amphetamines are substrates that do not only block the uptake of monoamines but also induce substrate efflux by promoting reverse transport. Reverse transport has been a focus of research for decades but its mechanistic basis still remains enigmatic. Recently, transporter-interacting proteins were found to regulate amphetamine-triggered reverse transport: calmodulin kinase IIα (αCaMKII) is a prominent example, because it binds the carboxyl terminus of DAT, phosphorylates its amino terminus, and supports amphetamine-induced substrate efflux in vitro. Here, we investigated whether, in vivo, the action of amphetamine was contingent on the presence of αCaMKII by recording the behavioral and neurochemical effects of amphetamine. Measurement of dopamine efflux in the dorsal striatum by microdialysis revealed that amphetamine induced less dopamine efflux in mice lacking αCaMKII. Consistent with this observation, the acute locomotor responses to amphetamine were also significantly blunted in αCaMKII-deficient mice. In addition, while the rewarding properties of amphetamine were preserved in αCaMKII-deficient mice, their behavioral sensitization to amphetamine was markedly reduced. Our findings demonstrate that amphetamine requires the presence of αCaMKII to elicit a full-fledged effect on DAT in vivo: αCaMKII does not only support acute amphetamine-induced dopamine efflux but is also important in shaping the chronic response to amphetamine