Truth-finding, procedural traditions and cultural trust in the Netherlands and England and Wales: when strengths become weaknesses

Criminal justice systems in different jurisdictions are based on different accounts as to how facts and truth are to be found. Because these accounts are often linked to procedural traditions they tend to draw normative weight from the past. Thus different criminal justice systems develop their own particular and critical points of trust where fundamental assumptions are made upon which the fact-finding capacity of the system is based. For jurisdictions from the inquisitorial tradition, trust is invested in the active truth-finding judge and the dossier. Thus in the Netherlands, the assumption is that thorough investigation led by an impartial prosecutor, the existence of a complete dossier and the active fact-finding role of the inquisitorial judge at trial preclude the necessity of strong defence rights and guarantee accurate truth-finding. Within the adversarial tradition in England and Wales, along with the jury and cross-examination at trial, the assumption is that, advance prosecution disclosure and independent active investigation by the defence provide a basis for strong defence narrative building and thus something like the equality of arms upon which accurate adversarial fact-finding is thought to depend. But what if the investigation in the Netherlands is neither thorough nor impartial, the dossier is incomplete and/or the judge unwilling to fulfil an active role? And what if the defence in England and Wales in fact lack the capacity or will either to conduct active independent pre-trial investigations or to make sense of the ‘unused materials’ disclosed by the prosecution? At this point, cultural trust in what are seen as systemic strengths can conceal points of weakness that not only render miscarriages of justice more likely but more difficult to identify and redress

A new parenting-based group intervention for young anxious children: results of a randomized controlled trial

Objective Despite recent advances, there are still no interventions that have been developed for the specific treatment of young children who have anxiety disorders. This study examined the impact of a new, cognitive–behaviorally based parenting intervention on anxiety symptoms. Method Families of 74 anxious children (aged 9 years or less) took part in a randomized controlled trial, which compared the new 10-session, group-format intervention with a wait-list control condition. Outcome measures included blinded diagnostic interview and self-reports from parents and children. Results Intention-to-treat analyses indicated that children whose parent(s) received the intervention were significantly less anxious at the end of the study than those in the control condition. Specifically, 57% of those receiving the new intervention were free of their primary disorder, compared with 15% in the control condition. Moreover, 32% of treated children were free of any anxiety diagnosis at the end of the treatment period, compared with 6% of those in the control group. Treatment gains were maintained at 12-month follow-up. Conclusions This new parenting-based intervention may represent an advance in the treatment of this previously neglected group. Clinical trial registration information: Anxiety in Young Children: A Randomized Controlled Trial of a New Cognitive-Behaviourally Based Parenting Intervention; http://www.isrctn.org/; ISRCTN12166762

Accurate measurement of 5-methylcytosine and 5-hydroxymethylcytosine in human cerebellum DNA by oxidative bisulfite on an array (OxBS-array).

The Infinium 450K Methylation array is an established tool for measuring methylation. However, the bisulfite (BS) reaction commonly used with the 450K array cannot distinguish between 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC). The oxidative-bisulfite assay disambiguates 5mC and 5hmC. We describe the use of oxBS in conjunction with the 450K array (oxBS-array) to analyse 5hmC/5mC in cerebellum DNA. The "methylation" level derived by the BS reaction is the combined level of 5mC and 5hmC at a given base, while the oxBS reaction gives the level of 5mC alone. The level of 5hmC is derived by subtracting the oxBS level from the BS level. Here we present an analysis method that distinguishes genuine positive levels of 5hmC at levels as low as 3%. We performed four replicates of the same sample of cerebellum and found a high level of reproducibility (average r for BS = 98.3, and average r for oxBS = 96.8). In total, 114,734 probes showed a significant positive measurement for 5hmC. The range at which we were able to distinguish 5hmC occupancy was between 3% and 42%. In order to investigate the effects of multiple replicates on 5hmC detection we also simulated fewer replicates and found that decreasing the number of replicates to two reduced the number of positive probes identified by > 50%. We validated our results using qPCR in conjunction with glucosylation of 5hmC sites followed by MspI digestion and we found good concordance with the array estimates (r = 0.94). This experiment provides a map of 5hmC in the cerebellum and a robust dataset for use as a standard in future 5hmC analyses. We also provide a novel method for validating the presence of 5hmC at low levels, and highlight some of the pitfalls associated with measuring 5hmC and 5mC.S. Balasubramanian is a Senior Investigator of The Wellcome Trust and the Balasubramanian group is core-funded by Cancer Research UK. We would like to thank Tobias Ost and Christine Clark of Cambridge Epigenetix Ltd. for valuable discussions and development of the method.This article was originally published in PLOS ONE (Field SF, Beraldi D, Bachman M, Stewart SK, Beck S, Balasubramanian S, PLoS ONE 2015, 10(2): e0118202. doi:10.1371/journal.pone.0118202

The association between anthropometric variables, functional movement screen scores and 100 m freestyle swimming performance in youth swimmers

This study examined the association between anthropometric variables, Functional Movement Screen (FMS) scores and 100 m freestyle swimming performance in early adolescent swimmers. Fifty competitive, national level, youth swimmers (21 males, 29 females, mean age ± SD = 13.5 ± 1.5 years, age range 11–16 years) performed an “all-out” 100 m freestyle (front crawl) swim as fast as they could in a 50 m pool. A median divide for 100 m timed swim was also used to divide the sample into faster or slower groups. Height, body mass, skinfolds and limb lengths were also assessed. Maturation was calculated by proxy using anthropometric measures and participants also undertook the FMS as a measure of functional performance. Backwards linear regression indicated a significant model (p = 0.0001, Adjusted R2 = 0.638) explaining 63.8% of the variance in swim performance with total sum of skinfolds, upper leg length, lower leg length, hand length and total height significantly contributing to the model. Swimmers who were classed as fast had lower total sum of skinfolds (p = 0.005) and higher total FMS score (p = 0.005) compared to their slower peers. In summary, this study indicates that anthropometric variables significantly explained the variance in 100 m freestyle swimming performance in youth swimmers

Resolving the contributions of the membrane-bound and periplasmic nitrate reductase systems to nitric oxide and nitrous oxide production in Salmonella enterica serovar Typhimurium

The production of cytotoxic nitric oxide (NO) and conversion into the neuropharmacological agent and potent greenhouse gas nitrous oxide (N2O) is linked with anoxic nitrate catabolism by Salmonella enterica serovar Typhimurium. Salmonella can synthesize two types of nitrate reductase: a membrane-bound form (Nar) and a periplasmic form (Nap). Nitrate catabolism was studied under nitrate-rich and nitrate-limited conditions in chemostat cultures following transition from oxic to anoxic conditions. Intracellular NO production was reported qualitatively by assessing transcription of the NO-regulated genes encoding flavohaemoglobin (Hmp), flavorubredoxin (NorV) and hybrid cluster protein (Hcp). A more quantitative analysis of the extent of NO formation was gained by measuring production of N2O, the end-product of anoxic NO-detoxification. Under nitrate-rich conditions, the nar, nap, hmp, norV and hcp genes were all induced following transition from the oxic to anoxic state, and 20% of nitrate consumed in steady-state was released as N2O when nitrite had accumulated to millimolar levels. The kinetics of nitrate consumption, nitrite accumulation and N2O production were similar to those of wild-type in nitrate-sufficient cultures of a nap mutant. In contrast, in a narG mutant, the steady-state rate of N2O production was ~30-fold lower than that of the wild-type. Under nitrate-limited conditions, nap, but not nar, was up-regulated following transition from oxic to anoxic metabolism and very little N2O production was observed. Thus a combination of nitrate-sufficiency, nitrite accumulation and an active Nar-type nitrate reductase leads to NO and thence N2O production, and this can account for up to 20% of the nitrate catabolized

Explaining, interpreting, and prescribing: some tensions and dilemmas in the comparative analysis of youth justice cultures

This chapter reflects on the implications of a cross‐cultural empirical research study on youth justice in Italy and Wales for transnational prescription of good practice. It examines the challenges in doing comparative studies which isolate the influence of particular elements of criminal justice regimes. Such analysis may seem well suited to transnational policy prescription in that particular elements are more easily transposed than whole systems. But institutional categories and practice may be so culturally imbedded that it becomes very difficult to understand their influence outside those particular cultural contexts. The article goes on to examine the potential (and the limitations) for transnational policy prescription of more holistic interpretive approaches to explanation rooted in analysis of legal cultures. It concludes that such approaches can expand the range of possible policy choices in terms of transnational prescription but cannot offer a means to predict their precise effects

Hard Scattering Factorization from Effective Field Theory

In this paper we show how gauge symmetries in an effective theory can be used to simplify proofs of factorization formulae in highly energetic hadronic processes. We use the soft-collinear effective theory, generalized to deal with back-to-back jets of collinear particles. Our proofs do not depend on the choice of a particular gauge, and the formalism is applicable to both exclusive and inclusive factorization. As examples we treat the pi-gamma form factor (gamma gamma* -> pi^0), light meson form factors (gamma* M -> M), as well as deep inelastic scattering (e- p -> e- X), Drell-Yan (p pbar -> X l+ l-), and deeply virtual Compton scattering (gamma* p -> gamma(*) p).Comment: 35 pages, 4 figures, typos corrected, journal versio