Flottledsåterställning i norra Sverige : effekter på habitatkvalitet och populationstätheter av juvenil öringar (Salmo trutta L.)

In an effort to evaluate the effects of restorations of float ways on population density of stream dwelling fish in relation to the changes in habitat, I studied the physical habitat restored and channalized sites and their population density of brown trout (Salmo trutta L.) in three streams in the municipality of Sorsele. The differences between the channalized and the restored sites where most noticeable regarding substrate which where generally coarser in the restored sites. The analyses showed that the proportion of habitat that where suitable to the age class 0+ where significantly lower in the restored sites compared to the channalized sites. Regarding age class ≥2+, no statistical differences in habitat quality were found. For age class 1+ the proportion of good habitat was significantly higher in restored than in channalized sites. Only the age class 1+ showed a significantly higher population density in restored sites compared to the channalized sites. A possible reason to why there where no positive response for the other age classes might be the diversified need of habitat of the three age classes. It's also possible that the lack of positive response to restoration might be explained by a bottleneck situation in the streams, i.e. the habitat improvement for one age class is levelled out by a reduction of habitat quality for another age class.I syfte att utvärdera effekterna av flottledsrestaureringar på populations täthet av strömlevande fisk och relatera detta till förändringar av habitatet studerade jag restaurerade och kanaliserade sträckor samt tätheten av öring (Salmo trutta L.) i tre vattendrag i Sorsele kommun. Skillnaderna mellan de kanaliserade och de restaurerade sträckorna var mest markant när det gäller substratet som överlag var grövre i restaurerade sträckor. Analysen visade att andelen av habitaten som var lämpliga för åldersklassen 0+ var signifikant lägre i restaurerade än i kanaliserade sträckor. För åldersklassen ≥2+ kunde dock inga statistiska skillnader i habitat kvalitet påvisas. För åldersklassen 1+ var andel av lämpligt habitat signifikant högre i restaurerade än i kanaliserade sträckor. Endast åldersklassen 1+ visade\ud en signifikant högre täthet i restaurerade i jämförelse med kanaliserade sträckor. En trolig orsak till utebliven positiv respons för de övriga åldergrupperna är det diversifierade behovet av habitat som de tre berörda ålderklasserna prefererar. Det är också möjligt att bristen på positiv respons på restaurering kan bero på att det finns en flaskhalssituation inom vattendragen, då förbättringar för vissa årsklasser tas ut av att det saknas förbättringar för de andra årsklasserna

Abortion services and providers in Canada in 2019: results of a national survey.

BACKGROUND: Since 2016, abortion care has undergone several important changes, particularly related to the provision of medical abortion using mifepristone. We aimed to document characteristics of the abortion care workforce in Canada after the update of clinical practice guidelines of mifepristone use for medical abortion. METHODS: We conducted a national, web-based, anonymized, bilingual (English/French) survey. We collected demographics and clinical care characteristics of physicians and nurse practitioners who provided abortion care in 2019. Between July and December 2020, we distributed the survey through professional organizations, including The College of Family Physicians of Canada and The Society of Obstetricians and Gynaecologists of Canada. We present descriptive statistics. RESULTS: Overall, 465 respondents representing all 10 provinces and 3 territories in Canada completed the survey. Of these, 388 (83.4%), including 30 nurse practitioners, provided first-trimester medical abortion, of which 350 (99.4%) used mifepristone. Two hundred and nineteen (47.1%) respondents provided first-trimester surgical abortion, 109 (23.4%) provided second-trimester surgical abortion and 115 (24.7%) provided second- or third-trimester medical abortion. Half of respondents reported fewer than 5 years of experience with any abortion care. Respondents reported providing a total of 48 509 abortions in 2019, including 32 345 (66.7%) first-trimester surgical abortions and 13 429 (27.7%) first-trimester medical abortions. In Quebec, only 1918 (12.5%) of reported abortions were first-trimester medical abortions. Primary care providers provided 34 540 (71.2%) of the total abortions. First-trimester medical abortions represented 44.4% (n = 2334) of all abortions in rural areas, as opposed to 25.6% (n = 11 067) in urban areas. INTERPRETATION: The increased availability of medical abortion facilitates abortion access, especially in primary care and rural settings, and where surgical abortion is not available. Rejuvenation of the workforce is a critical contributor to equitable access to abortion services

Forskning och innovation Årsrapport 2023

Årsrapport för forskning och innovation 2023. Beskriver verksamheten i siffror samt en sida med fördjupning per område/portfölj.

Forskning och innovation Årsrapport 2024

Årsrapport för forskning och innovation 2024. Beskriver verksamheten i siffror samt en sida med fördjupning per område/portfölj.

A new approach to detect membrane proteins : Loading of marker molecules into liposomes for detection of single membrane proteins

Membrane proteins are essential to the function of biological systems. The ability to map the placement and abundance of these proteins can facilitate several improvements within both medicine and biology. This project aims to construct a new method to map specific membrane proteins in tissue by loading marker molecules into liposomes decorated with targeting molecules for detection in mass spectrometry imaging (MSI). Loaded liposomes decorated with targeting molecules would make detection of one individual membrane protein possible in the MSI. This project resulted in a loading protocol of the marker molecule n-methyl,n-ethylbenzylamine (EMBA) into liposomes. The initial loading of EMBA could be determined to be higher than the detection limit of EMBA in solution using MSI. However, due to leakage, mainly during the post insertion during decorating of the liposomes, the amount of loaded EMBA is decreased which made the detection of liposomes in the MSI more difficult. The investigation of the possibility do detect a single loaded liposome in the MSI instrument was carried out both in solution and immobilised. Preliminary results show that EMBA from the loaded liposomes can be detected in the MSI. The immobilization technique needs further refinement due to disruption of the liposome layer as a result of drying during MSI measurements. The obtained results are a great advancement towards a new technique to map membrane proteins in actual tissue using a liposome system and MSI

Forskning och innovation Årsrapport 2024

Årsrapport för forskning och innovation 2024. Beskriver verksamheten i siffror samt en sida med fördjupning per område/portfölj.

A new approach to detect membrane proteins : Loading of marker molecules into liposomes for detection of single membrane proteins

Membrane proteins are essential to the function of biological systems. The ability to map the placement and abundance of these proteins can facilitate several improvements within both medicine and biology. This project aims to construct a new method to map specific membrane proteins in tissue by loading marker molecules into liposomes decorated with targeting molecules for detection in mass spectrometry imaging (MSI). Loaded liposomes decorated with targeting molecules would make detection of one individual membrane protein possible in the MSI. This project resulted in a loading protocol of the marker molecule n-methyl,n-ethylbenzylamine (EMBA) into liposomes. The initial loading of EMBA could be determined to be higher than the detection limit of EMBA in solution using MSI. However, due to leakage, mainly during the post insertion during decorating of the liposomes, the amount of loaded EMBA is decreased which made the detection of liposomes in the MSI more difficult. The investigation of the possibility do detect a single loaded liposome in the MSI instrument was carried out both in solution and immobilised. Preliminary results show that EMBA from the loaded liposomes can be detected in the MSI. The immobilization technique needs further refinement due to disruption of the liposome layer as a result of drying during MSI measurements. The obtained results are a great advancement towards a new technique to map membrane proteins in actual tissue using a liposome system and MSI

A new approach to detect membrane proteins : Loading of marker molecules into liposomes for detection of single membrane proteins

Membrane proteins are essential to the function of biological systems. The ability to map the placement and abundance of these proteins can facilitate several improvements within both medicine and biology. This project aims to construct a new method to map specific membrane proteins in tissue by loading marker molecules into liposomes decorated with targeting molecules for detection in mass spectrometry imaging (MSI). Loaded liposomes decorated with targeting molecules would make detection of one individual membrane protein possible in the MSI. This project resulted in a loading protocol of the marker molecule n-methyl,n-ethylbenzylamine (EMBA) into liposomes. The initial loading of EMBA could be determined to be higher than the detection limit of EMBA in solution using MSI. However, due to leakage, mainly during the post insertion during decorating of the liposomes, the amount of loaded EMBA is decreased which made the detection of liposomes in the MSI more difficult. The investigation of the possibility do detect a single loaded liposome in the MSI instrument was carried out both in solution and immobilised. Preliminary results show that EMBA from the loaded liposomes can be detected in the MSI. The immobilization technique needs further refinement due to disruption of the liposome layer as a result of drying during MSI measurements. The obtained results are a great advancement towards a new technique to map membrane proteins in actual tissue using a liposome system and MSI