Management of Anthracnose in Soybean Using Fungicide

Experiments on soybean (Glycine max L. Meril) were carried out aiming to control anthracnose (pod blight) caused by fungus, Colletotrichum truncatum with five treatments represented by different fungicidal sprays against control receiving no spray with three replicates of each under field conditions during two consecutive years from 2012 to 2013. In 2012, the higher Percent Disease Control (PDC) and Percent Yield Increase (PYI) were estimated in plot treated with SAAF (Carbendazim 12% + Mancozeb 63%) followed by Mancozeb fungicides. The mean Pod Infection (PI) was low in plots treated with SAAF followed by Mancozeb. Almost similar trends of disease control were observed in 2013. The lower Percent Disease Index (PDI) was 46.25% and mean PI was 29.67% with higher yield value of 2431.25 kg/ha obtained from the plots sprayed with SAAF then by Mancozeb. The results showed that, the combined treatment with fungicides, SAAF followed by Mancozeb were effective to control anthracnose or pod blight disease of soybean to increase the yield.Journal of Nepal Agricultural Research Council Vol.1 2015 pp.29-3

Functional and Radiological Outcome Following Imil Nailing for Floating Knee

Introduction:  The term "Floating Knee" denoted fractures that occur simultaneously on the same side of the tibia and femur, resulting in the knee joint flailing. Typically caused by high-energy traumas, like motor vehicle accidents or falls from height, these injuries present challenges due to related consequences like vascular injuries, compartment syndrome, and ligament damage. The study aimed to evaluate various surgical treatments for floating knee injuries, considering factors influencing prognosis and complications. Patients and Methods: A prospective survey of 15 patients with Frazer type 1 floating knee injuries, conducted from April 2022-2023, involved clinical and radiographic assessments. Exclusions encompass skeletally immature patients, those unfit for surgery, extensive soft tissue injuries, knee ligament involvement, intraarticular fractures, and pathological fractures. All patients underwent thorough preoperative evaluations, with treatment including intramedullary nailing. Postoperative rehabilitation and follow-up were integral components of the study. Results: The study involved 15 patients (mean age 30.9 years) with floating knee injuries, predominantly because of RTAs (Road Traffic Accidents). Intramedullary nailing for the femur, as well as tibia fractures, exhibited an average surgery duration of 2 hours 32 minutes and an average blood loss of 270 ml. Complications, including knee stiffness and infections, were managed effectively. Follow-up assessments according to Karlstrom\u27s criteria revealed excellent results in 20%, good in 60%, and acceptable in 20% of cases. Conclusion:The floating knee injury involves complexities impacting prognosis beyond simultaneous fractures of the femur and tibia. A meticulous initial assessment, prioritizing the identification of life-threatening related injuries, is crucial. Surgical fixation, preferably through intramedullary nailing, along with early and intensive postoperative rehabilitation, is recommended for improved functional and radiological outcomes

Metal Ceramic Segmented Ring Transducer under Deep Submergence Conditions

Segmented ring transducers are widely used for low frequency, broadband, deep submergence applications. These transducers can be made out of piezoceramic wedges or slabs and metallic wedges. Higher diameter, low frequency transducers are generally made out of piezoceramic slabs and metal wedges due to ease of manufacture and low cost. In this paper, metal ceramic segmented ring transducers are modelled using ATILA, a finite element software for the design of underwater transducers. Transducer variants were modelled with different wedge and piezoceramic materials. Transducers modelled were manufactured, assembled and tested. Various stages of manufacture like piezoceramic stacking, transducer assembly, pre-stressing with fibre winding, and encapsulation are explained. Acoustic performances of the transducers manufactured were measured in an open tank and inside a pressurised vessel from 10 bar to 70 bar. Performance parameters like resonance frequency, transmitting voltage response and directivity were measured. Results indicate that the transducer has usable bandwidth of about two octaves and stable response. One of the transducers was also tested in a high pressure test facility at 600 bar to check its pressure withstanding capability

Cerebral amyloid angiopathy in the aetiology and immunotherapy of Alzheimer disease

Amyloid is deposited in the walls of arteries and capillaries as cerebral amyloid angiopathy (CAA) in the brains of older individuals and of those with Alzheimer disease (AD). CAA in AD reflects an age-related failure of elimination of amyloid-beta (Aβ) from the brain along perivascular lymphatic drainage pathways. In the absence of conventional lymphatic vessel in the brain, interstitial fluid and solutes drain from the brain to cervical lymph nodes along narrow basement membranes in the walls of capillaries and arteries, a pathway that is largely separate from the cerebrospinal fluid. In this review we focus on the pathology and pathogenesis of CAA, its role in the aetiology of AD and its impact on immunotherapy for AD. The motive force for lymphatic drainage of the brain appears to be generated by arterial pulsations. Failure of elimination of Aβ along perivascular pathways coincides with a reduction in enzymic degradation of Aβ, reduced absorption of Aβ into the blood and age-related stiffening of artery walls that appears to reduce the motive force for lymphatic drainage. Reduced clearances of Aβ and CAA are associated with the accumulation of insoluble and soluble Aβs in the brain in AD and the probable loss of homeostasis of the neuronal environment due to retention of soluble metabolites within the brain. Tau metabolism may also be affected. Immunotherapy has been successful in removing insoluble plaques of Aβ from the brain in AD but with little effect on cognitive decline. One major problem is the increase in CAA in immunised patients that probably prevents the complete removal of Aβ from the brain. Increased knowledge of the physiology and structural and genetic aspects of the lymphatic drainage of Aβ from the brain will stimulate the development of therapeutic strategies for the prevention and treatment of AD

Filarial Lymphedema Is Characterized by Antigen- Specific Th1 and Th17 Proinflammatory Responses and a Lack of Regulatory T Cells

Background: Lymphatic filariasis can be associated with development of serious pathology in the form of lymphedema, hydrocele, and elephantiasis in a subset of infected patients. Methods and Findings: To elucidate the role of CD4+ T cell subsets in the development of lymphatic pathology, we examined specific sets of cytokines in individuals with filarial lymphedema in response to parasite antigen (BmA) and compared them with responses from asymptomatic infected individuals. We also examined expression patterns of Toll-like receptors (TLR1–10) and Nod-like receptors (Nod1, Nod2, and NALP3) in response to BmA. BmA induced significantly higher production of Th1-type cytokines—IFN-c and TNF-a—in patients with lymphedema compared with asymptomatic individuals. Notably, expression of the Th17 family of cytokines—IL-17A, IL-17F, IL-21, and IL-23—was also significantly upregulated by BmA stimulation in lymphedema patients. In contrast, expression of Foxp3, GITR, TGFb, and CTLA-4, known to be expressed by regulatory T cells, was significantly impaired in patients with lymphedema. BmA also induced significantly higher expression of TLR2, 4, 7, and 9 as well Nod1 and 2 mRNA in patients with lymphedema compared with asymptomatic controls. Conclusion: Our findings implicate increased Th1/Th17 responses and decreased regulatory T cells as well as regulation of Toll- and Nod-like receptors in pathogenesis of filarial lymphedema

Selection of antigenically advanced variants of seasonal influenza viruses

Influenza viruses mutate frequently, necessitating constant updates of vaccine viruses. To establish experimental approaches that may complement the current vaccine strain selection process, we selected antigenic variants from human H1N1 and H3N2 influenza virus libraries possessing random mutations in the globular head of the haemagglutinin protein (which includes the antigenic sites) by incubating them with human and/or ferret convalescent se

Mixed Th1 and Th2 Mycobacterium tuberculosis-specific CD4 T cell responses in patients with active pulmonary tuberculosis from Tanzania.

Mycobacterium tuberculosis (Mtb) and helminth infections elicit antagonistic immune effector functions and are co-endemic in several regions of the world. We therefore hypothesized that helminth infection may influence Mtb-specific T-cell immune responses. We evaluated the cytokine profile of Mtb-specific T cells in 72 individuals with pulmonary TB disease recruited from two Sub-Saharan regions with high and moderate helminth burden i.e. 55 from Tanzania (TZ) and 17 from South Africa (SA), respectively. We showed that Mtb-specific CD4 T-cell functional profile of TB patients from Tanzania are primarily composed of polyfunctional Th1 and Th2 cells, associated with increased expression of Gata-3 and reduced expression of T-bet in memory CD4 T cells. In contrast, the cytokine profile of Mtb-specific CD4 T cells of TB patients from SA was dominated by single IFN-γ and dual IFN-γ/TNF-α and associated with TB-induced systemic inflammation and elevated serum levels of type I IFNs. Of note, the proportion of patients with Mtb-specific CD8 T cells was significantly reduced in Mtb/helminth co-infected patients from TZ. It is likely that the underlying helminth infection and possibly genetic and other unknown environmental factors may have caused the induction of mixed Th1/Th2 Mtb-specific CD4 T cell responses in patients from TZ. Taken together, these results indicate that the generation of Mtb-specific CD4 and CD8 T cell responses may be substantially influenced by environmental factors in vivo. These observations may have major impact in the identification of immune biomarkers of disease status and correlates of protection

Allele-specific enhancer interaction at the Peg3 imprinted domain

Copyright: © 2019 Kim et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The parental allele specificity of mammalian imprinted genes has been evolutionarily well conserved, although its functional constraints and associated mechanisms are not fully understood. In the current study, we generated a mouse mutant with switched active alleles driving the switch from paternal-to-maternal expression for Peg3 and the maternal-to-paternal expression for Zim1. The expression levels of Peg3 and Zim1, but not the spatial expression patterns, within the brain showed clear differences between wild type and mutant animals. We identified putative enhancers localized upstream of Peg3 that displayed allele-biased DNA methylation, and that also participate in allele-biased chromosomal conformations with regional promoters. Most importantly, these data suggest for the first time that long-distance enhancers may contribute to allelic expression within imprinted domains through allele-biased interactions with regional promoters