Drowsy Driver Detection System

Driver weariness is one of the key causes of road mishaps in the world. Detecting the drowsiness of the driver can be one of the surest ways of quantifying driver fatigue. In this project we aim to develop an archetype drowsiness detection system. This mechanism works by monitoring the eyes of the driver and sounding an alarm when he/she feels heavy eyed. The system so constructed is a non-intrusive real-time observing system. The primacy is on improving the safety of the driver. In this mechanism the eye blink of the driver is detected. If the driver’s eyes remain closed for more than a certain span of time, the driver is believed to be tired and an alarm is sounded. The programming for this is carried out in OpenCV using the Haar cascade library for the detection of facial features

Drowsy Driver Detection System (DDDS)

Driver weariness is one of the key causes of road mishaps in the world. Detecting the drowsiness of the driver can be one of the surest ways of quantifying driver fatigue. In this project we have developed an archetype drowsiness detection system. This mechanism works by monitoring the eyes of the driver and sounding an alarm when he/she feels heavy eyed. The system constructed is a non-intrusive real-time perceiving system. The priority is on improving the safety of the driver. In this mechanism the eye blink of the driver is detected. If the driver?s eyes remain closed for greater than a certain period of time, the driver is deemed to be tired and an alarm is sounded. The programming for this is carried out in OpenCV using the Haar cascade library for the detection of facial features

Biodiversity of leaf litter fungi in streams along a latitudinal gradient

Global patterns of biodiversity have emerged for soil microorganisms, plants and animals, and the extraordinary significance of microbial functions in ecosystems is also well established. Virtually unknown, however, are large- scale patterns of microbial diversity in freshwaters, although these aquatic ecosystems are hotspots of biodiversity and biogeochemical processes. Here we report on the first large-scale study of biodiversity of leaf-litter fungi in streams along a latitudinal gradient unravelled by Illumina sequencing. The study is based on fungal commu- nities colonizing standardized plant litter in 19 globally distributed stream locations between 69°N and 44°S. Fungal richness suggests a hump-shaped distribution along the latitudinal gradient. Strikingly, community com- position of fungi was more clearly related to thermal preferences than to biogeography. Our results suggest that identifying differences in key environmental drivers, such as temperature, among taxa and ecosystem types is critical to unravel the global patterns of aquatic fungal diversity

Global patterns and drivers of ecosystem functioning in rivers and riparian zones

AbstractRiver ecosystems receive and process vast quantities of terrestrial organic carbon, the fate of which depends strongly on microbial activity. Variation in and controls of processing rates, however, are poorly characterized at the global scale. In response, we used a peer-sourced research network and a highly standardized carbon processing assay to conduct a global-scale field experiment in greater than 1000 river and riparian sites. We found that Earth’s biomes have distinct carbon processing signatures. Slow processing is evident across latitudes, whereas rapid rates are restricted to lower latitudes. Both the mean rate and variability decline with latitude, suggesting temperature constraints toward the poles and greater roles for other environmental drivers (e.g., nutrient loading) toward the equator. These results and data set the stage for unprecedented “next-generation biomonitoring” by establishing baselines to help quantify environmental impacts to the functioning of ecosystems at a global scale.Abstract River ecosystems receive and process vast quantities of terrestrial organic carbon, the fate of which depends strongly on microbial activity. Variation in and controls of processing rates, however, are poorly characterized at the global scale. In response, we used a peer-sourced research network and a highly standardized carbon processing assay to conduct a global-scale field experiment in greater than 1000 river and riparian sites. We found that Earth’s biomes have distinct carbon processing signatures. Slow processing is evident across latitudes, whereas rapid rates are restricted to lower latitudes. Both the mean rate and variability decline with latitude, suggesting temperature constraints toward the poles and greater roles for other environmental drivers (e.g., nutrient loading) toward the equator. These results and data set the stage for unprecedented “next-generation biomonitoring” by establishing baselines to help quantify environmental impacts to the functioning of ecosystems at a global scale

Global patterns and drivers of ecosystem functioning in rivers and riparian zones

Os ecossistemas fluviais recebem e processam grandes quantidades de carbono orgânico terrestre, cujo destino depende fortemente da atividade microbiana. A variação e o controle das taxas de processamento, no entanto, são mal caracterizados à escala global. Em resposta, utilizamos uma rede de pesquisa de pares e um ensaio de processamento de carbono altamente padronizado para conduzir um experimento de campo em escala global em mais de 1.000 locais fluviais e ribeirinhos. Descobrimos que os biomas da Terra têm assinaturas distintas de processamento de carbono. O processamento lento é evidente em todas as latitudes, enquanto as taxas rápidas são restritas às latitudes mais baixas. Tanto a taxa média como a variabilidade diminuem com a latitude, sugerindo restrições de temperatura em direção aos pólos e papéis maiores para outros fatores ambientais (por exemplo, carga de nutrientes) em direção ao equador. Estes resultados e dados preparam o terreno para uma “biomonitorização de próxima geração” sem precedentes, estabelecendo linhas de base para ajudar a quantificar os impactos ambientais no funcionamento dos ecossistemas à escala global.River ecosystems receive and process vast quantities of terrestrial organic carbon, the fate of which depends strongly on microbial activity. Variation in and controls of processing rates, however, are poorly characterized at the global scale. In response, we used a peer-sourced research network and a highly standardized carbon processing assay to conduct a global-scale field experiment in greater than 1000 river and riparian sites. We found that Earth’s biomes have distinct carbon processing signatures. Slow processing is evident across latitudes, whereas rapid rates are restricted to lower latitudes. Both the mean rate and variability decline with latitude, suggesting temperature constraints toward the poles and greater roles for other environmental drivers (e.g., nutrient loading) toward the equator. These results and data set the stage for unprecedented “next-generation biomonitoring” by establishing baselines to help quantify environmental impacts to the functioning of ecosystems at a global scale

Investigating the likely association between genetic ancestry and COVID-19 manifestations

AbstractBackgroundThe novel coronavirus: severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread rapidly throughout the world leading to catastrophic consequences. However, SARS-CoV-2 infection has shown discernible variability across the globe. While in some countries people are recovering relatively quickly, in others, recovery times have been comparatively longer and number of individuals succumbing to it are high. This variability in coronavirus disease 2019 (COVID-19) susceptibility is suggestive of a likely association between the genetic-make up of affected individuals modulated by their ancestry and the severity of COVID-19 manifestations.ObjectiveIn this study, we aimed to evaluate the potential association between an individual’s genetic ancestry and the extent of COVID-19 disease presentation employing Europeans as the case study. In addition, using a genome wide association (GWAS) approach we sought to discern the putative single nucleotide polymorphism (SNP) markers and genes that may be likely associated with differential COVID-19 manifestations by comparative analyses of the European and East Asian genomes.MethodTo this end, we employed 10,215 ancient and modern genomes across the globe assessing 597,573 SNPs obtained from the databank of Dr. David Reich, Harvard Medical School, USA to evaluate the likely correlation between European ancestry and COVID-19 manifestations. Ancestry proportions were determined using qpAdm program implemented in AdmixTools v5.1. Pearson’s correlation coefficient (r) between various ancestry proportions of European genomes and COVID-19 death/recovery ratio was calculated and its significance was statistically evaluated. Genome wide association study (GWAS) was performed in PLINK v1.9 to investigate SNPs with significant allele frequency variations among European and East Asian genomes that likely correlated with differential COVID-19 infectivity.ResultsWe found significant positive correlation (r=0.58, P=0.03) between West European hunter gatherers (WHG) ancestral fractions and COVID-19 death/recovery ratio for data as of 5th April 2020. This association discernibly amplified (r=0.77, P=0.009) upon reanalyses based on data as of 30th June 2020, removing countries with small sample sizes and adding those that are a bridge between Europe and Asia. Using GWAS we further identified 404 immune response related SNPs by comparing publicly available 753 genomes from various European countries against 838 genomes from various Eastern Asian countries. Prominently, we identified that SNPs associated with immune-system related pathways such as interferon stimulated antiviral response, adaptive and innate immune system and IL-6 dependent immune responses show significant differences in allele frequencies [Chi square values (≥1500; P≈0)] between Europeans and East Asians.ConclusionSo far, to the best of our knowledge, this is the first study investigating the likely association between host genetic ancestry and COVID-19 severity. These findings improve our overall understanding of the putative genetic modifiers of COVID-19 clinical presentation. We note that the development of effective therapeutics will benefit immensely from more detailed analyses of individual genomic sequence data from COVID-19 patients of varied ancestries.</jats:sec

Investigating the likely association between genetic ancestry and COVID-19 manifestation (Preprint)

UNSTRUCTURED The novel coronavirus 2019-nCoV/SARS-CoV-2 infection has shown discernible variability across the globe. While in some countries people are recovering relatively quicker, in others, recovery times have been comparatively longer and numbers of those succumbing to it high. In this study, we aimed to evaluate the likely association between an individual’s ancestry and the extent of COVID-19 manifestation employing Europeans as the case study. We employed 10,215 ancient and modern genomes across the globe assessing 597,573 single nucleotide polymorphisms (SNPs). Pearson’s correlation coefficient (r) between various ancestry proportions of European genomes and COVID-19 death/recovery ratio was calculated and its significance was statistically evaluated. We found significant positive correlation (p=0.03) between European Mesolithic hunter gatherers (WHG) ancestral fractions and COVID-19 death/recovery ratio and a marginally significant negative correlation (p=0.06) between Neolithic Iranian ancestry fractions and COVID-19 death/recovery ratio. We further identified 404 immune response related single nucleotide polymorphisms (SNPs) by comparing publicly available 753 genomes from various European countries against 838 genomes from various Eastern Asian countries in a genome wide association study (GWAS). Prominently, we identified that SNPs associated with Interferon stimulated antiviral response, Interferon-stimulated gene 15 mediated antiviral mechanism and 2′-5′ oligoadenylate synthase mediated antiviral response show large differences in allele frequencies between Europeans and East Asians. Overall, to the best of our knowledge, this is the first study evaluating the likely association between genetic ancestry and COVID-19 manifestation. While our current findings improve our overall understanding of the COVID-19, we note that the development of effective therapeutics will benefit immensely from more detailed analyses of individual genomic sequence data from COVID-19 patients of varied ancestries. </sec

Bioactive Potential of Lentinus squarrosulus and Termitomyces clypeatus from the Southwestern region of India

The antioxidants derived from biological sources are generally regarded as safe than synthetic ones owing to their less or no adverse effects. Wild mushrooms being a potential source of nutrients as well as antioxidants, bioactive properties of uncooked and cooked wood-inhabiting Lentinus squarrosulus andtermite mound-inhabiting Termitomyces clypeatus have been compared. Both mushrooms showed appreciable antioxidant potential, which is influenced by their bioactive principles (total phenolics, tannins, flavonoids and vitamin C) and proximal properties (crude protein, crude lipid, crude fibre and carbohydrates). These mushrooms were devoid of trypsin inhibition and hemagglutinin activity. Uncooked as well as cooked mushrooms with adequate nutritional components serve as sources of potential antioxidant to combat many human ailments. The present study and recent perspectives on nutraceuticals of macrofungi denote L. squarrosulus and T. clypeatus as the high-value nutraceutical sources in Southwestern India