A vaccine formulated with the major outer membrane protein can protect C3H/HeN, a highly susceptible strain of mice, from a Chlamydia muridarum genital challenge.

C3H/HeN female mice were vaccinated with native Chlamydia muridarum major outer membrane protein (MOMP), using Montanide+CpG or Alum+CpG as adjuvants. Negative control groups were immunized with ovalbumin (OVA) and the same adjuvants. As positive control, mice were inoculated intranasally with live Chlamydia. Mice were challenged in the ovarian bursa with 10(5) C. muridarum inclusion forming units. Six weeks after the genital challenge the animals were caged with male mice and monitored for pregnancy. Mice vaccinated with MOMP+Montanide+CpG developed high levels of C. muridarum-specific antibodies, with a high IgG2a/IgG1 ratio and neutralizing titres. Animals immunized using Alum+CpG had low antibody levels. Cellular immune responses were significantly higher in mice vaccinated with MOMP and Montanide+CpG, but not with Alum+CpG, when compared with negative controls. Following the genital challenge, only 20% (4/20) of mice vaccinated with MOMP+CpG+Montanide had positive vaginal cultures whereas 100% (9/9) of mice immunized with MOMP+CpG+Alum had positive cultures. Of the positive control animals inoculated with live Chlamydia only 15% (3/20) had positive vaginal cultures. In contrast, 100% (20/20) of mice immunized with OVA+CpG+Montanide, or minimal essential medium, had positive cultures. Following mating, 80% (16/20) of mice vaccinated with MOMP+CpG+Montanide, and 85% (17/20) of animals inoculated intranasally with live C. muridarum carried embryos in both uterine horns. No protection against infertility was observed in mice immunized with MOMP and CpG+Alum or OVA. In conclusion, this is the first time that a subunit vaccine has been shown to elicit a protective immune response in the highly susceptible C3H/HeN strain of mice against an upper genital challenge

Arachidonic Acid as a Possible Negative Feedback Inhibitor of Nicotinic Acetylcholine Receptors on Neurons

Neuronal acetylcholine receptors, being highly permeable to calcium, are likely to regulate calcium-dependent events in neurons. Arachidonic acid is a membrane-permeant second messenger that can be released from membrane phospholipids by phospholipases in a calcium-dependent manner. We show here that activation of neuronal acetylcholine receptors triggers release of 3H-arachidonic acid in a calcium-dependent manner from neurons preloaded with the fatty acid. Moreover, low concentrations of arachidonic acid reversibly inhibit the receptors and act most efficiently on receptors likely to have the highest permeability to calcium, namely receptors containing α7 subunits. Low concentrations of arachidonic acid also reversibly inhibit α7- containing receptors expressed in Xenopus oocytes following injection of α7 cRNA. The oocyte results indicate following injection of α7 cRNA. The oocyte results indicate that the inhibition is a feature of the receptors rather than a consequence of neuron-specific machinery. The inhibition is not mediated by specific metabolites of arachidonic acid because the effects can be mimicked by other fatty acids; their effectiveness correlates with their content of double bonds. In contrast to arachidonic effects on calcium currents, inhibition of neuronal nicotinic receptors by the fatty acid cannot be prevented by blocking production of free radicals or by inhibiting protein kinase C. An alternative mechanism is that arachidonic acid binds directly to the receptors or perturbs the local environment in such a manner as to constrain receptor function

Coherent States for Isospectral Hamiltonians

We show that for the strictly isospectral Hamiltonians, the corresponding coherent states are related by a unitary transformation. As an illustration, we discuss, the example of strictly isospectral one-dimensional harmonic oscillator Hamiltonians and the associated coherent states.Comment: RevTeX, 9 pages, no figure

How the presentation of patient information and decision-support advisories influences opioid prescribing behavior: A simulation study

ObjectiveThe United States faces an opioid crisis. Integrating prescription drug monitoring programs into electronic health records offers promise to improve opioid prescribing practices. This study aimed to evaluate 2 different user interface designs for prescription drug monitoring program and electronic health record integration.Materials and MethodsTwenty-four resident physicians participated in a randomized controlled experiment using 4 simulated patient cases. In the conventional condition, prescription opioid histories were presented in tabular format, and computerized clinical decision support (CDS) was provided via interruptive modal dialogs (ie, pop-ups). The alternative condition featured a graphical opioid history, a cue to visit that history, and noninterruptive CDS. Two attending pain specialists judged prescription appropriateness.ResultsParticipants in the alternative condition wrote more appropriate prescriptions. When asked after the experiment, most participants stated that they preferred the alternative design to the conventional design.ConclusionsHow patient information and CDS are presented appears to have a significant influence on opioid prescribing behavior

Annual reproductive cycle of the Rock Oyster Saccostrea cucullata (von Born)

The annual reproductive cycle of the rock oyster, Saccostrea cuculata (von Born) inhabiting Someshwar coast near Mangalore was investigated. The reproductive cycle commences with gametogenic activity during January - February, followed by gonadal development and maturation during March - May. Spawning is continuous from June to December, with two peaks, the first during late June to early September and the second during November to December

Larval development of the Rock Oyster Saccostrea cucullata (von Born)

The development of artificially fertilised eggs of the rock oyster, Saccostrea cucullata (von Born) was studied in the laboratory. The morphological and behavioural characteristics of the developing larvae were observed. A mixed diet of single celled algae consisting of Isochrysis galbana and Tetraselmis gracilis was fed to the larvae. The larval developmental stages observed include trochophore, D-veliger, late veliger, umbone stage, pediveJiger and plantigrade

Multi-Channel Inverse Scattering Problem on the Line: Thresholds and Bound States

We consider the multi-channel inverse scattering problem in one-dimension in the presence of thresholds and bound states for a potential of finite support. Utilizing the Levin representation, we derive the general Marchenko integral equation for N-coupled channels and show that, unlike to the case of the radial inverse scattering problem, the information on the bound state energies and asymptotic normalization constants can be inferred from the reflection coefficient matrix alone. Thus, given this matrix, the Marchenko inverse scattering procedure can provide us with a unique multi-channel potential. The relationship to supersymmetric partner potentials as well as possible applications are discussed. The integral equation has been implemented numerically and applied to several schematic examples showing the characteristic features of multi-channel systems. A possible application of the formalism to technological problems is briefly discussed.Comment: 19 pages, 5 figure

Phase shift effective range expansion from supersymmetric quantum mechanics

Supersymmetric or Darboux transformations are used to construct local phase equivalent deep and shallow potentials for $\ell \neq 0$ partial waves. We associate the value of the orbital angular momentum with the asymptotic form of the potential at infinity which allows us to introduce adequate long-distance transformations. The approach is shown to be effective in getting the correct phase shift effective range expansion. Applications are considered for the $^1P_1$ and $^1D_2$ partial waves of the neutron-proton scattering.Comment: 6 pages, 3 figures, Revtex4, version to be publised in Physical Review

Computational modeling of TC0583 as a putative component of the Chlamydia muridarum V-type ATP synthase complex and assessment of its protective capabilities as a vaccine antigen.

Numerous Chlamydia trachomatis proteins have been identified as potential subunit vaccines, of which the major outer-membrane protein (MOMP) has, so far, proven the most efficacious. Recently, subunit A of the V-type ATP synthase (ATPase; TC0582) complex was shown to elicit partial protection against infection. Computational modeling of a neighboring gene revealed a novel subunit of the V-type ATPase (TC0583). To determine if this newly identified subunit could induce protection and/or enhance the partial protection provided by subunit A alone, challenge studies were performed using a combination of these recombinant proteins. The TC0583 subunit alone and concurrently with TC0582, was used to vaccinate BALB/c mice utilizing CpG-1826 and Montanide ISA 720 VG as adjuvants. Vaccinated animals were challenged intranasally with Chlamydia muridarum and the course of the infection was followed. Mice immunized with individual antigens showed minimal alleviation of body weight reduction; however, mice immunized with TC0583 and TC0582 in combination, displayed weight loss levels close to those observed with MOMP. Importantly, immunization with a combination of recombinant subunit proteins reduced chlamydial inclusion forming units by approximately a log-fold. These protection levels support that, these highly conserved Chlamydia proteins, in combination with other antigens, may serve as potential vaccine candidates