Turfgrass research report 1995

Postemergence herbicide efficacy on crabgrass / John R. Street and Renee M. Stewart -- Preemergence herbicide efficacy on crabgrass / John R. Street and Renee M. Stewart --Preemergent common chickweed weed control evaluation / William Pound -- Preemergent purple dead nettle weed control evaluation / William Pound -- General turfgrass broadleaf weed control evaluation / William Pound -- Finale/Roundup herbicide demonstration evaluation / William Pound -- Ground ivy control evaluation / William Pound -- Manage yellow nutsedge control evaluation / William Pound -- Alternative Turflon solvent tolerance evaluation / William Pound -- Wild violet control evaluation / William Pound --Leaf spot study, 1995 / Joe Rimelspach, Karl Danneberger and Jill Taylor -- Leaf spot control study - Galena, Ohio / Joe Rimelspach, Karl Danneberger and Jill Taylor -- Red thread control study, 1995 / Joe Rimelspach, Karl Danneberger -- Brown patch study, 1995 / Joe Rimelspach, Karl Danneberger, Joseph Vagnier and Jill Taylor -- Brown patch (Rhizoctonia solani) curative study / Joe Rimelspach, Karl Danneberger and Jill Taylor -- Preventive dollar spot (Sclerotinia homoeocarpa) control study, 1995 / Joe Rimelspach, Karl Danneberger, Joseph Vagnier and Jill Taylor -- Take-All control study / Joe Rimelspach and Karl Danneberger -- Yellow tuft study, 1995 / Joe Rimelspach, Karl Danneberger, Joseph Vagnier and Jill Taylor -- Summer patch control study / Rob Golembiewski, Joe Rimelspach and Karl Danneberger -- Sentinel and Daconil Ultrex gallonage study: influence on dollar spot control / Karl Danneberger and Jill Taylor -- Pink snow mold control study, 1994-1995 / Jill Taylor, Joe Rimelspach and Karl Danneberger -- Control of black cutworm, Agrotis ipsilon (Hufnagel), and sod webworms (Pyralidae: Crambinae) on short cut bentgrass, Agrostis palustris Hudson-1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Control of black cutworm, Agrotis ipsilon (Hufnagel), and sod webworms (Pyralidae: Crambinae) on short cut bentgrass, Agrostis palustris Hudson using spinosad formulations -1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Applications of insecticides for control of second generation hairy chinch bugs in turfgrass -1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Preventive application of insecticides for control of black turfgrass Ataenius larvae on golf course fairways, 1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Spring application of chemical and biological insecticides for control of overwintered white grubs in turfgrass, 1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Influence of post-treatment irrigation on the efficacy of RH 0345 and Merit applied at the time of egg hatch for control of Japanese beetle larvae in turf, 1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Application of various insecticides for preventive control of Japanese Beetle larvae in turfgrass, 1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Chemical and biological insecticides applied for control of white grubs in turfgrass, 1995 / David J. Shetlar, Harry D. Niemczyk and Kevin T. Power -- Nitrogen source, rate, and timing effect on Kentucky bluegrass / John R. Street and Renee M. Stewart -- Natural organic source evaluation on a Kentucky bluegrass-perennial ryegrass mixture / John R. Street and Renee M. Stewart -- Nitrogen source, rate, and timing effect on Kentucky bluegrass / John R. Street and Renee M. Stewart -- Polymer-coated nitrogen source effect on Kentucky bluegrass / John R. Street and Renee M. Stewart -- 1993 NTEP bentgrass test (fairway/tee) / Jill A. Taylor -- 1993 NTEP fineleaf fescue test / Jill Taylor -- 1994 NTEP perennial ryegrass test / Jill Taylor -- 'Primo' growth regulator evaluation on creeping bentgrass / William Pound -- Bermudagrass management study / John Street and Jill Taylor -- Electrophoretic evaluation of esterase isozymes from turfgrass seed blends and mixtures / G. E. Bell, M. B. McDonald Jr. and T. K. Danneberger -- Computer Imaging of Electrophoretic Gels / G. E. Bell, M. B. McDonald Jr., T. K. Danneberger and S. K. St. Martin -- Evaluation of Kentucky bluegrass blends using isoelectric focusing and computer imaging / G. E. Bell, M. B. McDonald Jr. and T. K. Danneberger -- Identification of RAFLP markers in perennial ryegrass / Patricia M. Sweeney and Karl Danneberger -- RAPD analysis of dry turfgrass seed / Patricia Sweeney, Robert Golembiewski and Karl Danneberge

B Cells Regulate Neutrophilia during Mycobacterium tuberculosis Infection and BCG Vaccination by Modulating the Interleukin-17 Response

We have previously demonstrated that B cells can shape the immune response to Mycobacterium tuberculosis, including the level of neutrophil infiltration and granulomatous inflammation at the site of infection. The present study examined the mechanisms by which B cells regulate the host neutrophilic response upon exposure to mycobacteria and how neutrophilia may influence vaccine efficacy. To address these questions, a murine aerosol infection tuberculosis (TB) model and an intradermal (ID) ear BCG immunization mouse model, involving both the μMT strain and B cell-depleted C57BL/6 mice, were used. IL (interleukin)-17 neutralization and neutrophil depletion experiments using these systems provide evidence that B cells can regulate neutrophilia by modulating the IL-17 response during M. tuberculosis infection and BCG immunization. Exuberant neutrophilia at the site of immunization in B cell-deficient mice adversely affects dendritic cell (DC) migration to the draining lymph nodes and attenuates the development of the vaccine-induced Th1 response. The results suggest that B cells are required for the development of optimal protective anti-TB immunity upon BCG vaccination by regulating the IL-17/neutrophilic response. Administration of sera derived from M. tuberculosis-infected C57BL/6 wild-type mice reverses the lung neutrophilia phenotype in tuberculous μMT mice. Together, these observations provide insight into the mechanisms by which B cells and humoral immunity modulate vaccine-induced Th1 response and regulate neutrophila during M. tuberculosis infection and BCG immunization. © 2013 Kozakiewicz et al

Self-affirmation improves performance on tasks related to executive functioning

Objectives: The current study explored the effect of self-affirmation on two aspects of performance that have been related to executive functioning: working memory (assessed by a 2-back task) and inhibition (assessed by a Stroop task). The goal was to establish whether self-affirmation improved performance on these tasks. Method: Participants (N = 83) were randomized to either a self-affirmation or a control task and then completed the computerized tasks, in a fixed sequence. Results: Self-affirmed participants performed better than non-affirmed participants on both tasks. Conclusion: Self-affirmation can improve aspects of performance related to executive functioning. This finding may help to explain the wide range of beneficial effects that self-affirmation can have on cognition and behavior

cGAL, a temperature-robust GAL4–UAS system for Caenorhabditis elegans

The GAL4–UAS system is a powerful tool for manipulating gene expression, but its application in Caenorhabditis elegans has not been described. Here we systematically optimize the system's three main components to develop a temperature-optimized GAL4–UAS system (cGAL) that robustly controls gene expression in C. elegans from 15 to 25 °C. We demonstrate this system's utility in transcriptional reporter analysis, site-of-action experiments and exogenous transgene expression; and we provide a basic driver and effector toolkit

Lipid metabolic perturbation is an early-onset phenotype in adult spinster mutants: a Drosophila model for lysosomal storage disorders

Intracellular accumulation of lipids and swollen dysfunctional lysosomes are linked to several neurodegenerative diseases, including lysosomal storage disorders (LSD). Detailed characterization of lipid metabolic changes in relation to the onset and progression of neurodegeneration is currently missing. We systematically analyzed lipid perturbations in spinster (spin) mutants, a Drosophila model of LSD-like neurodegeneration. Our results highlight an imbalance in brain ceramide and sphingosine in the early stages of neurodegeneration, preceding the accumulation of endomembranous structures, manifestation of altered behavior, and buildup of lipofuscin. Manipulating levels of ceramidase and altering these lipids in spin mutants allowed us to conclude that ceramide homeostasis is the driving force in disease progression and is integral to spin function in the adult nervous system. We identified 29 novel physical interaction partners of Spin and focused on the lipid carrier protein, Lipophorin (Lpp). A subset of Lpp and Spin colocalize in the brain and within organs specialized for lipid metabolism (fat bodies and oenocytes). Reduced Lpp protein was observed in spin mutant tissues. Finally, increased levels of lipid metabolites produced by oenocytes in spin mutants allude to a functional interaction between Spin and Lpp, underscoring the systemic nature of lipid perturbation in LSD

Mapping hydroxyl variability throughout the global remote troposphere via synthesis of airborne and satellite formaldehyde observations

The hydroxyl radical (OH) fuels tropospheric ozone production and governs the lifetime of methane and many other gases. Existing methods to quantify global OH are limited to annual and global-to-hemispheric averages. Finer resolution is essential for isolating model deficiencies and building process-level understanding. In situ observations from the Atmospheric Tomography (ATom) mission demonstrate that remote tropospheric OH is tightly coupled to the production and loss of formaldehyde (HCHO), a major hydrocarbon oxidation product. Synthesis of this relationship with satellite-based HCHO retrievals and model-derived HCHO loss frequencies yields a map of total-column OH abundance throughout the remote troposphere (up to 70% of tropospheric mass) over the first two ATom missions (August 2016 and February 2017). This dataset offers unique insights on near-global oxidizing capacity. OH exhibits significant seasonality within individual hemispheres, but the domain mean concentration is nearly identical for both seasons (1.03 ± 0.25 × 10^6 cm^(−3)), and the biseasonal average North/South Hemisphere ratio is 0.89 ± 0.06, consistent with a balance of OH sources and sinks across the remote troposphere. Regional phenomena are also highlighted, such as a 10-fold OH depression in the Tropical West Pacific and enhancements in the East Pacific and South Atlantic. This method is complementary to budget-based global OH constraints and can help elucidate the spatial and temporal variability of OH production and methane loss

Experimental and modelling study of fatigue crack initiation in an aluminium beam with a hole under 4-point bending

Slip band formation and crack initiation during cyclic fatigue were investigated by in-situ experiments and non-local CPFEM simulations systematically. Experimental techniques including EBSD, digital image correlation (DIC) and SEM have been used to obtain consistent grain orientations, local strains, as well as the locations where slip bands and micro-cracks form on the sample surface. The realistic microstructure based on the EBSD map has been generated and used for finite element modelling. An advanced non-local crystal plasticity model, which considers the isotropic and kinematic hardening of the plastic strain gradient, has been adopted. The simulation results match well the corresponding experimental results. It was found that total strain and averaged slip on all slip systems, combined with accumulated slip on specific slip planes help predict the location and orientation of slip bands and micro-crack initiation correctly. Furthermore, a fatigue indicating parameter based on competition between maximum slip and the total slip has been proposed to reproduce the experimental observations

De novo CCND2 mutations leading to stabilization of cyclin D2 cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome

Activating mutations in genes encoding phosphatidylinositol 3-kinase (PI3K)-AKT pathway components cause megalencephaly-polymicrogyria-polydactyly-hydrocephalus syndrome (MPPH, OMIM 603387). Here we report that individuals with MPPH lacking upstream PI3K-AKT pathway mutations carry de novo mutations in CCND2 (encoding cyclin D2) that are clustered around a residue that can be phosphorylated by glycogen synthase kinase 3β (GSK-3β). Mutant CCND2 was resistant to proteasomal degradation in vitro compared to wild-type CCND2. The PI3K-AKT pathway modulates GSK-3β activity, and cells from individuals with PIK3CA, PIK3R2 or AKT3 mutations showed similar CCND2 accumulation. CCND2 was expressed at higher levels in brains of mouse embryos expressing activated AKT3. In utero electroporation of mutant CCND2 into embryonic mouse brains produced more proliferating transfected progenitors and a smaller fraction of progenitors exiting the cell cycle compared to cells electroporated with wild-type CCND2. These observations suggest that cyclin D2 stabilization, caused by CCND2 mutation or PI3K-AKT activation, is a unifying mechanism in PI3K-AKT–related megalencephaly syndromes