Oregon Wine Board Meeting Minutes September 11, 2012

These meeting minutes list individuals in attendance and missing at the September 11, 2012 Oregon Wine Board (OWB) meeting, held via conference call. Dewey Weddington provided a marketing update focused on planning for Oregon Wine Month and the Oregon Wine Industry Symposium. The meeting also included discussion of the 2011-2012 year-end financial review and a presentation of the budget for the following year. The meeting lasted 2 hours 6 minutes, and the Board went into Executive Session after the meeting was adjourned

Kinematical structure of the circumstellar environments of galactic B[e]-type stars

High resolution line profiles are presented for selected forbidden and permitted emission lines of a sample of galactic B[e]-type stars. The spectral resolution corresponds to 5-7 km/s with the exception of some line profiles which were observed with a resolution of 9-13 km/s. All Ha profiles are characterized by a narrow split or single emission component with a width of about 150-250 km/s (FWHM) and broad wings with a full width of ~1000-2000 km/s. The Ha profiles can be classified into three groups: double-peaked profiles representing the majority, single-peaked emission-line profiles, and normal P Cygni-type profiles. The forbidden lines exhibit in most cases double-peaked profiles. The split forbidden line profiles have peak separations of as little as 10 km/s. The ratio of violet to red emission peak intensities, V/R, is predominantly smaller or equal to 1. Theoretical profiles were calculated for the optically thin case. A latitude-dependent stellar wind with a radial expansion and a velocity decreasing from the pole to the equator was adopted. In addition an equatorial dust ring with various optical depths was assumed. This model can explain split lines and line asymmetries observed in some stars. Moreover, the V/R ratios can be understood in terms of this model. The comparison of the observed line profiles with the models thus confirms the assumption of disk-like line-formation regions as commonly adopted for B[e]-type stars.Comment: Astronomy & Astrophysics, in pres

Microbial genomic taxonomy

A need for a genomic species definition is emerging from several independent studies worldwide. In this commentary paper, we discuss recent studies on the genomic taxonomy of diverse microbial groups and a unified species definition based on genomics. Accordingly, strains from the same microbial species share >95% Average Amino Acid Identity (AAI) and Average Nucleotide Identity (ANI), >95% identity based on multiple alignment genes, 70% in silico Genome-to-Genome Hybridization similarity (GGDH). Species of the same genus will form monophyletic groups on the basis of 16S rRNA gene sequences, Multilocus Sequence Analysis (MLSA) and supertree analysis. In addition to the established requirements for species descriptions, we propose that new taxa descriptions should also include at least a draft genome sequence of the type strain in order to obtain a clear outlook on the genomic landscape of the novel microbe. The application of the new genomic species definition put forward here will allow researchers to use genome sequences to define simultaneously coherent phenotypic and genomic groups

IDENTIFICATION AND PLANT INTERACTION OF A PHYLLOBACTERIUM SP, A PREDOMINANT RHIZOBACTERIUM OF YOUNG SUGAR-BEET PLANTS

The second most abundant bacterium on the root surface of young sugar beet plants was identified as a Phyllobacterium sp. (Rhizobiaceae) based on a comparison of the results of 39 conventional identification tests, 167 API tests, 30 antibiotic susceptibility tests, and sodium dodecyl sulfate-polyacrylamide gel electrophoretic fingerprints of total cellular proteins with type strains of Phyllobacterium myrsinacearum and Phyllobacterium rubiacearum. It was found on 198 of 1,100 investigated plants between the 2nd and 10th leaf stage on three different fields in Belgium and one field in Spain. Densities ranged from 2 × 10(4) to 2 × 10(8) CFU/g of root. Five isolates exerted a broad-spectrum in vitro antifungal activity. DNA-DNA hybridizations showed that Phyllobacterium sp. does not contain DNA sequences that are homologous with the attachment genes chvA, chvB, the transferred-DNA (T-DNA) hormone genes iaaH and ipt from Agrobacterium tumefaciens, iaaM from A. tumefaciens and Pseudomonas savastanoi, or the nitrogenase genes nifHDK from Klebsiella pneumoniae. Phyllobacterium sp. produces indolylacetic acid in in vitro cultures and induces auxinlike effects when cocultivated with callus tissue of tobacco. When Phyllobacterium sp. was transformed with a Ti plasmid derivative, it gained the capacity to induce tumors on Kalanchoe daigremontiana. The potential role of Phyllobacterium sp. in this newly recognized niche is discussed

Diversity of culturable moderately halophilic and halotolerant bacteria in a marsh and two salterns a protected ecosystem of Lower Loukkos (Morocco)

To study the biodiversity of halophilic bacteria in a protected wetland located in Loukkos (Northwest, Morocco), a total of 124 strains were recovered from sediment samples from a marsh and salterns. 120 isolates (98%) were found to be moderately halophilic bacteria; growing in salt ranges of 0.5 to 20%. Of 124 isolates, 102 were Gram-positive while 22 were Gram negative. All isolates were identified based on 16S rRNA gene phylogenetic analysis and characterized phenotypically and by screening for extracellular hydrolytic enzymes. The Gram-positive isolates were dominated by the genus Bacillus (89%) and the others were assigned to Jeotgalibacillus, Planococcus, Staphylococcus and Thalassobacillus. The Gram negative isolates were dominated by the genus Vibrio (41%) and the others were assigned to Halomonas, Psychrobacter, Marinobacterium, Pseudoalteromonas, Salinivibrio and Photobacterium. The growth of strains obtained under different physico-chemical conditions and the screening for hydrolytic enzymes showed a high diversity even within the same species

IAMBEE : a web-service for the identification of adaptive pathways from parallel evolved clonal populations

IAMBEE is a web server designed for the Identification of Adaptive Mutations in Bacterial Evolution Experiments (IAMBEE). Input data consist of genotype information obtained from independently evolved clonal populations or strains that show the same adapted behavior (phenotype). To distinguish adaptive from passenger mutations, IAMBEE searches for neighborhoods in an organism-specific interaction network that are recurrently mutated in the adapted populations. This search for recurrently mutated network neighborhoods, as proxies for pathways is driven by additional information on the functional impact of the observed genetic changes and their dynamics during adaptive evolution. In addition, the search explicitly accounts for the differences in mutation rate between the independently evolved populations. Using this approach, IAMBEE allows exploiting parallel evolution to identify adaptive pathways. The web-server is freely available at http://bioinformatics.intec.ugent.be/iambee/ with no login requirement

<i>Vibrio rotiferianus</i> sp. nov., isolated from cultures of the rotifer <i>Brachionus plicatilis</i>

Five Gram-negative bacterial strains, oxidase-positive, motile by means of more than one polar flagella, facultative anaerobe, arginine dihydrolase-negative, lysine- and ornithine decarboxylase-positive, sensitive to the vibriostatic agent O/129, were isolated from a flow-through rotifer culture system in Gent, Belgium, and previously characterized by fluorescent amplified fragment length polymorphism. Comparison of the 16S rDNA sequence of strain LMG 21460T indicated close relationships (~99 % similarity) to Vibrio campbellii, Vibrio harveyi, Vibrio alginolyticus and Vibrio parahaemolyticus. However, DNA hybridization experiments revealed similarity values below 70 % with its closest species V. campbellii and V. harveyi. Additionally, the analysed strains differ from related Vibrio species by the utilization of melibiose and production of acid from L-arabinose and amygdalin. Among the strains analysed, differences were observed in some phenotypic characters, particularly susceptibility to ampicillin, polymyxin B and amikacin, and urease activity. The major fatty acids identified were 16:0, 18:1 ?7c, 14:0, 12:0 3-OH and 18:0. Vibrio rotiferianus sp. nov. is proposed, with type strain LMG 21460T (=CAIM 577T); it has a DNA G+C content of 44·5 ± 0·01 mol%

SwSt 1: an O-rich planetary nebula around a C-rich central star

The hydrogen-deficient carbon-rich [WCL] type central star HD167362 and its oxygen-rich planetary nebula (PN) SwSt~1 are investigated. The nebular chemistry might indicate a recent origin for the carbon-rich stellar spectrum. Its stellar and nebular properties might therefore provide further understanding of the origin of the [WCL] central star class. The UV-IR stellar spectra are modelled with state of the codes and show ~40kK central star with a wind and a C/O~3, indicative of efficient third dredge-up. The synthetic stellar flux distribution is used to model the high density, compact PN, which has a solar C/O ratio, is still enshrouded by 1200K and 230K dust shells and, reported here for the first time, in molecular hydrogen. Although it appears that the change in C/O ratio has been recent, the published spectroscopy since 1895 has been re-examined and no clear spectral change is seen. If an event occurred that has turned it into a hydrogen-deficient central star, it did not happen in the last 100 years.Comment: 31 pages, 19 figures (some are gif files), MNRAS in pres