Standortkonversion als Chance

Die Finanzierung der Bundeswehr-Reform bereitet dem Bundesverteidigungsminister beträchtliche Schwierigkeiten. Unter anderem sollen mittels der Schließung von Bundeswehrstandorten Finanzierungsreserven mobilisiert werden. Können die erwarteten Finanzmittel realisiert werden? Wie reagieren die Betroffenen auf die Standortkonversion

Formation of a carcinogenic aromatic amine from an azo dye by human skin bacteria in vitro

Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.Azo dyes represent the major class of dyestuffs. They are metabolised to the corresponding amines by liver enzymes and the intestinal microflora following incorporation by both experimental animals and humans. For safety evaluation of the dermal exposure of consumers to azo dyes from wearing coloured textiles, a possible cleavage of azo dyes by the skin microflora should be considered since, in contrast to many dyes, aromatic amines are easily absorbed by the skin. A method for measuring the ability of human skin flora to reduce azo dyes was established. In a standard experiment, 361011 cells of a culture of Staphylococcus aureus wereincubatedinsyntheticsweat (pH 6.8, final volume 20 mL) at 288C for 24 h with Direct Blue 14 (C.I. 23850, DB 14). The reaction products were extracted and analysed using HPLC. The reduction product o-tolidine (3,3'-dimethylbenzidine, OT) could indeed be detected showing that the strain used was able to metabolise DB 14 to the corresponding aromatic amine. In addition to OT, two further metabolites of DB 14 were detected. Using mass spectrometry they were identified as 3,3'-dimethyl-4-amino-4'-hydroxybiphenyl and 3,3'-di methyl-4-aminobiphenyl. The ability to cleave azo dyes seems to be widely distributed among human skin bacteria, as, under these in vitro conditions, bacteria isolated from healthy human skin and human skin bacteria from strain collections also exhibited azo reductase activity. Further studies are in progress in order to include additional azo dyes and coloured textiles. At the moment, the meaning of the results with regard to consumer health cannot be finally assessed

Standortkonversion als Chance

Die Finanzierung der Bundeswehr-Reform bereitet dem Bundesverteidigungsminister beträchtliche Schwierigkeiten. Unter anderem sollen mittels der Schließung von Bundeswehrstandorten Finanzierungsreserven mobilisiert werden. Können die erwarteten Finanzmittel realisiert werden? Wie reagieren die Betroffenen auf die Standortkonversion? --

The Generation of Successive Unmarked Mutations and Chromosomal Insertion of Heterologous Genes in Actinobacillus pleuropneumoniae Using Natural Transformation

We have developed a simple method of generating scarless, unmarked mutations in Actinobacillus pleuropneumoniae by exploiting the ability of this bacterium to undergo natural transformation, and with no need to introduce plasmids encoding recombinases or resolvases. This method involves two successive rounds of natural transformation using linear DNA: the first introduces a cassette carrying cat (which allows selection by chloramphenicol) and sacB (which allows counter-selection using sucrose) flanked by sequences to either side of the target gene; the second transformation utilises the flanking sequences ligated directly to each other in order to remove the cat-sacB cassette. In order to ensure efficient uptake of the target DNA during transformation, A. pleuropneumoniae uptake sequences are added into the constructs used in both rounds of transformation. This method can be used to generate multiple successive deletions and can also be used to introduce targeted point mutations or insertions of heterologous genes into the A. pleuropneumoniae chromosome for development of live attenuated vaccine strains. So far, we have applied this method to highly transformable isolates of serovars 8 (MIDG2331), which is the most prevalent in the UK, and 15 (HS143). By screening clinical isolates of other serovars, it should be possible to identify other amenable strains

Density of states of helium droplets

Accurate analytical expressions for the state densities of liquid He-4 droplets are derived, incorporating the ripplon and phonon degrees of freedom. The microcanonical temperature and the ripplon angular momentum level density are also evaluated. The approach is based on inversions and systematic expansions of canonical thermodynamic properties.Comment: 20 pages, 5 figure

Design of one-dimensional Lambertian diffusers of light

We describe a method for designing a one-dimensional random surface that acts as a Lambertian diffuser. The method is tested by means of rigorous computer simulations and is shown to yield the desired scattering pattern.Comment: 6 pages, 2 figure

Weyl asymptotics: From closed to open systems

We present microwave experiments on the symmetry reduced 5-disk billiard studying the transition from a closed to an open system. The measured microwave reflection signal is analyzed by means of the harmonic inversion and the counting function of the resulting resonances is studied. For the closed system this counting function shows the Weyl asymptotic with a leading exponent equal to 2. By opening the system successively this exponent decreases smoothly to an non-integer value. For the open systems the extraction of resonances by the harmonic inversion becomes more challenging and the arising difficulties are discussed. The results can be interpreted as a first experimental indication for the fractal Weyl conjecture for resonances.Comment: 9 pages, 7 figure

Expanded boundary integral method and chaotic time-reversal doublets in quantum billiards

We present the expanded boundary integral method for solving the planar Helmholtz problem, which combines the ideas of the boundary integral method and the scaling method and is applicable to arbitrary shapes. We apply the method to a chaotic billiard with unidirectional transport, where we demonstrate existence of doublets of chaotic eigenstates, which are quasi-degenerate due to time-reversal symmetry, and a very particular level spacing distribution that attains a chaotic Shnirelman peak at short energy ranges and exhibits GUE-like statistics for large energy ranges. We show that, as a consequence of such particular level statistics or algebraic tunneling between disjoint chaotic components connected by time-reversal operation, the system exhibits quantum current reversals.Comment: 18 pages, 8 figures, with 3 additional GIF animations available at http://chaos.fiz.uni-lj.si/~veble/boundary

Discrete Symmetries in the Weyl Expansion for Quantum Billiards

We consider two and three-dimensional quantum billiards with discrete symmetries. We derive the first terms of the Weyl expansion for the level density projected onto the irreducible representations of the symmetry group. As an illustration the method is applied to the icosahedral billiard. The paper was published in J. Phys. A /27/ (1994) 4317-4323Comment: 8 printed pages Latex fil

Spectral Statistics in Chaotic Systems with Two Identical Connected Cells

Chaotic systems that decompose into two cells connected only by a narrow channel exhibit characteristic deviations of their quantum spectral statistics from the canonical random-matrix ensembles. The equilibration between the cells introduces an additional classical time scale that is manifest also in the spectral form factor. If the two cells are related by a spatial symmetry, the spectrum shows doublets, reflected in the form factor as a positive peak around the Heisenberg time. We combine a semiclassical analysis with an independent random-matrix approach to the doublet splittings to obtain the form factor on all time (energy) scales. Its only free parameter is the characteristic time of exchange between the cells in units of the Heisenberg time.Comment: 37 pages, 15 figures, changed content, additional autho