Resistência extrema a duas estirpes do Potato virus Y (PVY) de batata transgênica, cv. Achat, expressando o gene da capa protéica do PVYO

The coat protein (CP) gene of the potato virus Y strain “o” (PVYO) was introduced into potato, cultivar Achat, via Agrobacterium tumefaciens-mediated transformation. Sixty three putative transgenic lines were challenged against the Brazilian strains PVY-OBR and PVY-NBR. An extremely resistant phenotype, against the two strains, was observed in one line, denominated 1P. No symptoms or positive ELISA results were observed in 16 challenged plants from this line. Another clone, named as 63P, showed a lower level of resistance. Southern blot analysis showed five copies of the CP gene in the extremely resistant line and at least three copies in the other resistant line. The stability of the integrated transgenes in the extreme resistant line was examined during several in vitro multiplications over a period of three years, with no modification in the Southern pattern was observed. The stability of the transgenes, the absence of primary infections and the relatively broad spectrum of resistance suggest that the extremely resistant line obtained in this work can be useful for agricultural purposes.O gene da capa protéica (CP) do Potato virus Y estirpe “o”, foi introduzido em batata cultivar Achat, via Agrobacterium tumefaciens. Sessenta e três linhas possivelmente transgênicas foram desafiadas com as estirpes brasileiras PVY-OBR e PVY-NBR. Uma linha apresentou extrema resistência às duas estirpes inoculadas, e foi denominado clone 1P. Não foram observados sintomas sistêmicos de infecção e as plantas foram negativas em Elisa. Outra linha, denominada clone 63P, mostrou algum nível de resistência. Análises por Southern blot indicaram a presença de pelo menos cinco cópias do gen CP no clone 1P e pelo menos três cópias no clone 63P. A estabilidade do gene introduzido no clone 1P foi avaliada durante três anos, após várias multiplicações in vitro. Não foram observadas mudanças no padrão do Southern blot. A estabilidade do transgene, na ausência de infecções primárias e relativo largo espectro de resistência sugerem que o clone 1P pode ser utilizado para fins comerciais.Fil: Romano, Eduardo. Embrapa Recursos Genéticos; BrasilFil: Ferreira, Adriana T.. Embrapa Hortaliças; BrasilFil: Dusi, André N.. Embrapa Hortaliças; BrasilFil: Proite, Karina. Embrapa Recursos Genéticos; BrasilFil: Buso, Jose A.. Embrapa Hortaliças; BrasilFil: Avila, Antonio C.. Embrapa Hortaliças; BrasilFil: Nishijima, Marta L.. Embrapa Hortaliças; BrasilFil: Nascimento, Adriana S.. Embrapa Hortaliças; BrasilFil: Bravo Almonacid, Fernando Felix. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Mentaberry, Alejandro Nestor. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones en Ingeniería Genética y Biología Molecular "Dr. Héctor N. Torres"; Argentina. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Monte, Damares. Embrapa Recursos Genéticos; BrasilFil: Campos, Magnolia A.. Embrapa Recursos Genéticos; BrasilFil: Melo, Paulo Eduardo. Embrapa Hortaliças; BrasilFil: Cattony, Monica K.. No especifica;Fil: Torres, Antonio C.. Embrapa Hortaliças; Brasi

A new FSA approach for in situ γ\gamma-ray spectroscopy

An increasing demand of environmental radioactivity monitoring comes both from the scientific community and from the society. This requires accurate, reliable and fast response preferably from portable radiation detectors. Thanks to recent improvements in the technology, $\gamma$-spectroscopy with sodium iodide scintillators has been proved to be an excellent tool for in-situ measurements for the identification and quantitative determination of $\gamma$-ray emitting radioisotopes, reducing time and costs. Both for geological and civil purposes not only $^{40}$K, $^{238}$U, and $^{232}$Th have to be measured, but there is also a growing interest to determine the abundances of anthropic elements, like $^{137}$Cs and $^{131}$I, which are used to monitor the effect of nuclear accidents or other human activities. The Full Spectrum Analysis (FSA) approach has been chosen to analyze the $\gamma$-spectra. The Non Negative Least Square (NNLS) and the energy calibration adjustment have been implemented in this method for the first time in order to correct the intrinsic problem related with the $\chi ^2$ minimization which could lead to artifacts and non physical results in the analysis. A new calibration procedure has been developed for the FSA method by using in situ $\gamma$-spectra instead of calibration pad spectra. Finally, the new method has been validated by acquiring $\gamma$-spectra with a 10.16 cm x 10.16 cm sodium iodide detector in 80 different sites in the Ombrone basin, in Tuscany. The results from the FSA method have been compared with the laboratory measurements by using HPGe detectors on soil samples collected in the different sites, showing a satisfactory agreement between them. In particular, the $^{137}$Cs isotopes has been implemented in the analysis since it has been found not negligible during the in-situ measurements.Comment: accepted by Science of Total Environment: 8 pages, 10 figures, 3 table

Preliminary results for the characterization of the radiological levels of rocks in Tuscany Region.

The environmental background levels of natural radiation due to the nuclides in rocks vary in significant amounts that depend on the geological and geomorphological features of a territory. The main source of terrestrial gamma-ray radiation exposure to humans is from 238U, 232Th decay chains and 40K decay. This paper reports a part of the results of the Research Project “Measurement of natural radioactivity and mapping of the radioisotope abundances of Tuscany Region”, which started at August 2008 and it is supported by founds of Tuscany Region. The aim of this project is to realize the thematic maps of radioactivity content and in particular of the abundances of eU1, eTh1 and 40K. These goals will be achieved by integrating the information from measurements on samples in laboratory with in-situ investigations and airborne surveys. The Legnaro National Laboratory (LNL) is the national leader for the design and realization of high-resolution gamma-ray spectrometers, portable and massive NaI(Tl) detectors. The MCA_Rad gamma-ray spectrometry system was designed and built up at LNL for measuring large amount of samples with a minimum attendance: these features fit perfectly with the requirements of this project. This system is able to measure any type of materials (solid, liquid, gas), and due to the high efficiency and its geometric symmetry, absolute activity measurements are possible with systematic errors below 5%. http://www.fe.infn.it/u/mantovani/CV/Proceedings/Bezzon_10b.pd

Glossario de biotecnologia vegetal.

bitstream/item/107353/1/CNPH-GLOSS.-DE-BIOTEC.-VEG.-00.pd

Mapping of natural radioelements using gamma-ray spectrometry: Tuscany Region case of study.

http://www.fe.infn.it/u/mantovani/CV/Proceedings/Bezzon_11a.pd

Análise de transferibilidade de primers microssatélites de Cucumis melo para Cucurbita moschata e Luffa cylindric.

bitstream/CENARGEN/29527/1/bp203.pd

Preliminary results for the characterization of the radiological levels of rocks in Tuscany Region

http://www.fe.infn.it/u/mantovani/CV/Proceedings/Bezzon_10a.pd

Environmental performances in green labels for hotels – a critical review

The global attention towards climate change has led national governments and the international community to the definition of plans aiming to reduce greenhouse gas emissions in all economic sectors. Recently, attention has focused also on the tourism sector, and especially on the lodging industry, which consumes high amounts of resources and energy to satisfy guests expectations in terms of offered services and comfort conditions. In this sector, eco-certifications or green labels are spreading, perceived as useful marketing tools to communicate the hoteliers’ environmental efforts to consumers, who are becoming more and more sensitive to ecological matters. However, the wide offer of green labels and the lack of appropriate information are contributing to increase costumers’ confusion and perception of real “green”. The present paper focuses its attention on a set of currently available tools to evaluate the environmental performances of hotels, in order to enquire if and to which extent they are able to inform about the sustainability of accommodation structures. Starting from the wide number of certification schemes available on the market, 19 multi-attribute, third-party green labels were compared, aiming to explore the role that energy efficiency measures play in the certification procedure

Mapa genético baseado em marcadores microssatélites e RAPD utilizando populações de melhoramento contrastante para teor de fibra no feijoeiro comum (Phaseolus vulgaris L.).

O objetivo deste trabalho foi à construção de um mapa genético do feijoeiro comum, utilizando populações de melhoramento contrastante para o teor de fibra com base em marcadores microssatélites e RAPD