Identification, surveillance and management of Aedes vexans in a flooded river valley in Nottinghamshire, United Kingdom

Aedes vexans is known to occur in large populations in riverine floodplains in much of Europe, where it can cause a significant biting nuisance and is often subject to large scale control strategies. Until recently it had only been reported in very small numbers in the United Kingdom. After receiving reports of nuisance biting near the river Idle, Nottinghamshire (East Midlands, England), mosquito surveillance was conducted over three years (2018-2020) using Mosquito Magnet adult traps. Ae. vexans was found in all years, in very high numbers, particularly in 2020, reaching a peak of almost 5,000 female mosquitoes per trap night, the highest reported density of trapped adult mosquitoes in the UK. Larval control was conducted in all years, and adult control in one year, however local peculiarities of flood and water management presents challenges and necessitates a multi-faceted approach. Strategies for further expansion of the control operation by developing strategies for water management, coupled with larval surveys to define the extent and seasonality of larval habitats, and application methods of Bacillus thuringiensis israelensis products are discussed

Detectionof gas hydrates infaults using azimuthal seismic velocity analysis,Vestnesa Ridge, W-Svalbard Margin

Accepted for publication in Journal of Geophysical Research. Solid Earth. Copyright 2020 American Geophysical Union. Further reproduction or electronic distribution is not permitted.Joint analysis of electrical resistivity and seismic velocity data is primarily used to detect the presence of gas hydrate‐filled faults and fractures. In this study, we present a novel approach to infer the occurrence of structurally‐controlled gas hydrate accumulations using azimuthal seismic velocity analysis. We perform this analysis using ocean‐bottom seismic (OBS) data at two sites on Vestnesa Ridge, W‐Svalbard Margin. Previous geophysical studies inferred the presence of gas hydrates at shallow depths (up to ~190‐195 m below the seafloor) in marine sediments of Vestnesa Ridge. We analyze azimuthal P‐wave seismic velocities in relation with steeply‐dipping near surface faults to study structural controls on gas hydrate distribution. This unique analysis documents directional changes in seismic velocities along and across faults. P‐wave velocities are elevated and reduced by ~0.06‐0.08 km/s in azimuths where the raypath plane lies along the fault plane in the gas hydrate stability zone (GHSZ) and below the base of the GHSZ, respectively. The resulting velocities can be explained with the presence of gas hydrate‐ and free gas‐filled faults above and below the base of the GHSZ, respectively. Moreover, the occurrence of elevated and reduced (>0.05 km/s) seismic velocities in groups of azimuths bounded by faults, suggests compartmentalization of gas hydrates and free gas by fault planes. Results from gas hydrate saturation modelling suggest that these observed changes in seismic velocities with azimuth can be due to gas hydrate saturated faults of thickness greater than 20 cm and considerably smaller than 300 cm

Modeling Activity and Target-Dependent Developmental Cell Death of Mouse Retinal Ganglion Cells Ex Vivo

Programmed cell death is widespread during the development of the central nervous system and serves multiple purposes including the establishment of neural connections. In the mouse retina a substantial reduction of retinal ganglion cells (RGCs) occurs during the first postnatal week, coinciding with the formation of retinotopic maps in the superior colliculus (SC). We previously established a retino-collicular culture preparation which recapitulates the progressive topographic ordering of RGC projections during early post-natal life. Here, we questioned whether this model could also be suitable to examine the mechanisms underlying developmental cell death of RGCs. Brn3a was used as a marker of the RGCs. A developmental decline in the number of Brn3a-immunolabelled neurons was found in the retinal explant with a timing that paralleled that observed in vivo. In contrast, the density of photoreceptors or of starburst amacrine cells increased, mimicking the evolution of these cell populations in vivo. Blockade of neural activity with tetrodotoxin increased the number of surviving Brn3a-labelled neurons in the retinal explant, as did the increase in target availability when one retinal explant was confronted with 2 or 4 collicular slices. Thus, this ex vivo model reproduces the developmental reduction of RGCs and recapitulates its regulation by neural activity and target availability. It therefore offers a simple way to analyze developmental cell death in this classic system. Using this model, we show that ephrin-A signaling does not participate to the regulation of the Brn3a population size in the retina, indicating that eprhin-A-mediated elimination of exuberant projections does not involve developmental cell death

Subanesthetic ketamine infusions for the treatment of children and adolescents with chronic pain: a longitudinal study

BACKGROUND: Chronic pain is common in children and adolescents and is often associated with severe functional disability and mood disorders. The pharmacological treatment of chronic pain in children and adolescents can be challenging, ineffective, and is mostly based on expert opinions and consensus. Ketamine, an N-methyl-D-aspartate receptor antagonist, has been used as an adjuvant for treatment of adult chronic pain and has been shown, in some instances, to improve pain and decrease opioid-requirement. We examined the effects of subanesthetic ketamine infusions on pain intensity and opioid use in children and adolescents with chronic pain syndromes treated in an outpatient setting. METHODS: Longitudinal cohort study of consecutive pediatric patients treated with subanesthetic ketamine infusions in a tertiary outpatient center. Outcome measurements included self-reported pain scores (numeric rating scale) and morphine-equivalent intake. RESULTS: Over a 15-month period, 63 children and adolescents (median age 15, interquartile range 12–17 years) with chronic pain received 277 ketamine infusions. Intravenous administration of subanesthetic doses of ketamine to children and adolescents on an outpatient basis was safe and not associated with psychotropic effects or hemodynamic perturbations. Overall, ketamine significantly reduced pain intensity (p <0.001) and yielded greater pain reduction in patients with complex regional pain syndrome (CRPS) than in patients with other chronic pain syndromes (p = 0.029). Ketamine-associated reductions in pain scores were the largest in postural orthostatic tachycardia syndrome (POTS) and trauma patients and the smallest in patients with chronic headache (p = 0.007). In 37 % of infusions, patients had a greater than 20 % reduction in pain score. Conversely, ketamine infusions did not change overall morphine-equivalent intake (p = 0.3). CONCLUSIONS: These data suggest that subanesthetic ketamine infusion is feasible in an outpatient setting and may benefit children and adolescents with chronic pain. Further, patients with CRPS, POTS, and a history of trauma-related chronic pain are more likely to benefit from this therapeutic modality