On a initial value problem arising in mechanics

We study initial value problem for a system consisting of an integer order and distributed-order fractional differential equation describing forced oscillations of a body attached to a free end of a light viscoelastic rod. Explicit form of a solution for a class of linear viscoelastic solids is given in terms of a convolution integral. Restrictions on storage and loss moduli following from the Second Law of Thermodynamics play the crucial role in establishing the form of the solution. Some previous results are shown to be special cases of the present analysis

Protein-Protein Fusion Catalyzed by Sortase A

Chimeric proteins boast widespread use in areas ranging from cell biology to drug delivery. Post-translational protein fusion using the bacterial transpeptidase sortase A provides an attractive alternative when traditional gene fusion fails. We describe use of this enzyme for in vitro protein ligation and report the successful fusion of 10 pairs of protein domains with preserved functionality — demonstrating the robust and facile nature of this reaction

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The following report provides results from a geochemical survey for Thomas Range-Wasatch, Utah. Field and laboratory data are presented for 15 groundwater and 79 stream sediment samples

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The following report gives detailed results of the geochemical survey taken in Buffalo-Lignite, South Dakota. Field and laboratory data are presented for 163 groundwater and 660 stream sediment samples collected within the Custer and Slim Buttes project areas

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Documentation outlining characteristics of field data samples taken in the Buffalo-Lignite detail area

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Results of the Chinati Mountains project area of the detailed geochemical survey fro Trans-Pecos, Texas are reported

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Results of the Stillwell Mountains project area of the detailed geochemical survey for Trans-Pecos, Texas are reported

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Results of the Dryden project area of the detailed geochemical survey for Trans-Pecos, Texas are reported

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Documentation outlining characteristics of field data samples taken in the Stillwell Mountains project area

Covalent Attachment of Proteins to Solid Supports and Surfaces via Sortase-Mediated Ligation

BACKGROUND: There is growing interest in the attachment of proteins to solid supports for the development of supported catalysts, affinity matrices, and micro devices as well as for the development of planar and bead based protein arrays for multiplexed assays of protein concentration, interactions, and activity. A critical requirement for these applications is the generation of a stable linkage between the solid support and the immobilized, but still functional, protein. METHODOLOGY: Solid supports including crosslinked polymer beads, beaded agarose, and planar glass surfaces, were modified to present an oligoglycine motif to solution. A range of proteins were ligated to the various surfaces using the Sortase A enzyme of S. aureus. Reactions were carried out in aqueous buffer conditions at room temperature for times between one and twelve hours. CONCLUSIONS: The Sortase A transpeptidase of S. aureus provides a general, robust, and gentle approach to the selective covalent immobilization of proteins on three very different solid supports. The proteins remain functional and accessible to solution. Sortase mediated ligation is therefore a straightforward methodology for the preparation of solid supported enzymes and bead based assays, as well as the modification of planar surfaces for microanalytical devices and protein arrays