Enhancement of collagen deposition and cross-linking by coupling lysyl oxidase with bone morphogenetic protein-1 and its application in tissue engineering

Cultured cell-derived extracellular matrices (ECM)-based biomaterials exploit the inherent capacity of cells to create highly sophisticated supramolecular assemblies. However, standard cell culture conditions are far from ideal given the fact that the diluted microenvironment does not favor the production of ECM components, a circumstance particularly relevant for collagen. An incomplete conversion of procollagen by C-proteinase/bone morphogenetic protein 1 (BMP1) has been proposed to severely limit in vitro collagen deposition. BMP1 also catalyzes the proteolytic activation of the precursor of the collagen cross-linking enzyme, lysyl oxidase (LOX) to yield the active form, suggesting a deficit in cross-linking activity under standard conditions. We hypothesized that the implementation of fibroblast cultures with LOX and BMP1 may be an effective way to increase collagen deposition. To test it, we have generated stable cell lines overexpressing LOX and BMP1 and studied the effect of supernatants enriched in LOX and BMP1 on collagen synthesis and deposition from fibroblasts. Herein, we demonstrate that the supplementation with LOX and BMP1 strongly increased the deposition of collagen onto the insoluble matrix at the expense of the soluble fraction in the extracellular medium. Using decellularization protocols, we also show that fibroblast-derived matrices regulate adipogenic and osteogenic differentiation of human mesenchymal stem cells (MSC), and this effect was modulated by LOX/BMP1. Collectively, these data demonstrate that we have developed a convenient protocol to enhance the capacity of in vitro cell cultures to deposit collagen in the ECM, representing this approach a promising technology for application in tissue engineeringTis work was supported by grants from Ministerio de Economía y Competitividad (Plan Nacional de I+D+I: SAF2012-34916, and SAF2015-65679-R to F.R-P

Material Dependence of the Wire-Particle Casimir Interaction

We study the Casimir interaction between a metallic cylindrical wire and a metallic spherical particle by employing the scattering formalism. At large separations, we derive the asymptotic form of the interaction. In addition, we find the interaction between a metallic wire and an isotropic atom, both in the non-retarded and retarded limits. We identify the conditions under which the asymptotic Casimir interaction does not depend on the material properties of the metallic wire and the particle. Moreover, we compute the exact Casimir interaction between the particle and the wire numerically. We show that there is a complete agreement between the numerics and the asymptotic energies at large separations. For short separations, our numerical results show good agreement with the proximity force approximation

Linear Response Theory and Optical Conductivity of Floquet Topological Insulators

Motivated by the quest for experimentally accessible dynamical probes of Floquet topological insulators, we formulate the linear response theory of a periodically driven system. We illustrate the applications of this formalism by giving general expressions for optical conductivity of Floquet systems, including its homodyne and heterodyne components and beyond. We obtain the Floquet optical conductivity of specific driven models, including two-dimensional Dirac material such as the surface of a topological insulator, graphene, and the Haldane model irradiated with circularly or linearly polarized laser, as well as semiconductor quantum well driven by an ac potential. We obtain approximate analytical expressions and perform numerically exact calculations of the Floquet optical conductivity in different scenarios of the occupation of the Floquet bands, in particular, the diagonal Floquet distribution and the distribution obtained after a quench. We comment on experimental signatures and detection of Floquet topological phases using optical probes.Comment: 16 pages, 10 figure

Altitude calibration of an F100, S/N P680063, turbofan engine

An airflow and thrust calibration of an F100 engine was conducted in coordination with a flight test program to study airframe-propulsion system integration characteristics of turbofan-powered high-performance aircraft. The tests were conducted with and without augmentation for a variety of simulated flight conditions with emphasis on the transonic regime. Test results for all conditions are presented in terms of corrected airflow and corrected gross thrust as functions of corrected fan speed for nonaugmented power and an augmented thrust ratio as a function of fuel-air ratio for augmented power. Comparisons of measured and predicted data are presented along with the results of an uncertainty analysis for both corrected airflow and gross thrust

Crosstalk Correction in Atomic Force Microscopy

Commercial atomic force microscopes usually use a four-segmented photodiode to detect the motion of the cantilever via laser beam deflection. This read-out technique enables to measure bending and torsion of the cantilever separately. A slight angle between the orientation of the photodiode and the plane of the readout beam, however, causes false signals in both readout channels, so-called crosstalk, that may lead to misinterpretation of the acquired data. We demonstrate this fault with images recorded in contact mode on ferroelectric crystals and present an electronic circuit to compensate for it, thereby enabling crosstalk-free imaging

Special issue on smart interactions in cyber-physical systems: Humans, agents, robots, machines, and sensors

In recent years, there has been increasing interaction between humans and non‐human systems as we move further beyond the industrial age, the information age, and as we move into the fourth‐generation society. The ability to distinguish between human and non‐human capabilities has become more difficult to discern. Given this, it is common that cyber‐physical systems (CPSs) are rapidly integrated with human functionality, and humans have become increasingly dependent on CPSs to perform their daily routines.The constant indicators of a future where human and non‐human CPSs relationships consistently interact and where they allow each other to navigate through a set of non‐trivial goals is an interesting and rich area of research, discovery, and practical work area. The evidence of con- vergence has rapidly gained clarity, demonstrating that we can use complex combinations of sensors, artificial intelli- gence, and data to augment human life and knowledge. To expand the knowledge in this area, we should explain how to model, design, validate, implement, and experiment with these complex systems of interaction, communication, and networking, which will be developed and explored in this special issue. This special issue will include ideas of the future that are relevant for understanding, discerning, and developing the relationship between humans and non‐ human CPSs as well as the practical nature of systems that facilitate the integration between humans, agents, robots, machines, and sensors (HARMS).Fil: Kim, Donghan. Kyung Hee University;Fil: Rodriguez, Sebastian Alberto. Universidad Tecnológica Nacional; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán; ArgentinaFil: Matson, Eric T.. Purdue University; Estados UnidosFil: Kim, Gerard Jounghyun. Korea University

Does the Galaxy-Halo Connection Vary with Environment?

SubHalo Abundance Matching (SHAM) assumes that one (sub)halo property, such as mass Mvir or peak circular velocity Vpeak, determines properties of the galaxy hosted in each (sub)halo such as its luminosity or stellar mass. This assumption implies that the dependence of Galaxy Luminosity Functions (GLFs) and the Galaxy Stellar Mass Function (GSMF) on environmental density is determined by the corresponding halo density dependence. In this paper, we test this by determining from an SDSS sample the observed dependence with environmental density of the ugriz GLFs and GSMF for all galaxies, and for central and satellite galaxies separately. We then show that the SHAM predictions are in remarkable agreement with these observations, even when the galaxy population is divided between central and satellite galaxies. However, we show that SHAM fails to reproduce the correct dependence between environmental density and g-r color for all galaxies and central galaxies, although it better reproduces the color dependence on environmental density of satellite galaxies.Comment: 21 pages, 11 figures. Accepted for publication in MNRA

Faint emission lines in the Galactic H II regions M16, M20 and NGC 3603

We present deep echelle spectrophotometry of the Galactic {\hii} regions M16, M20 and NGC 3603. The data have been taken with the Very Large Telescope Ultraviolet-Visual Echelle Spectrograph in the 3100 to 10400 \AA range. We have detected more than 200 emission lines in each region. Physical conditions have been derived using different continuum and line intensity ratios. We have derived He$^{+}$, C$^{++}$ and O$^{++}$ abundances from pure recombination lines as well as abundances from collisionally excited lines for a large number of ions of different elements. We have obtained consistent estimations of the temperature fluctuation parameter, {\ts}, using different methods. We also report the detection of deuterium Balmer lines up to D$\delta$ (M16) and to D$\gamma$ (M20) in the blue wings of the hydrogen lines, which excitation mechanism seems to be continuum fluorescence. The temperature fluctuations paradigm agree with the results obtained from optical CELs and the more uncertain ones from far IR fine structure CELs in NGC 3603, although, more observations covering the same volume of the nebula are necessary to obtain solid conclusions.Comment: 22 pages, 13 Tables, 7 Figures. Accepted for publication by MNRA