Burying Beetles (Coleoptera: Silphidae) of the Apostle Islands, Wisconsin: Species Diversity, Population Density and Body Size

Over 2400 burying beetles, representing six species (Nicrophorus defodiens, N. sayi, N orbicollis, N. tomentosus, N vespilloides, and N. pustulatus), were trapped from 27 June to 4 August, 1996 at nine study sites (3 small islands, 3 large islands, and 3 mainland locations) centered around the Apostle Islands National Lakeshore in northern Wisconsin. Species diversity was greatest on the mainland and least on the smallest islands « 600 ha). Nicrophorus defodiens, the smallest of the six species, was marginally overrepresented on the smallest islands. We hypothesize that this occurred because N. defodiens can maintain a larger population for a given resource base than its larger congeners. Estimates of population density for N. defodi­ens were made on the two smallest islands (Raspberry and Devils) and ranged from 16-24/ha. On the small and isolated Devils Island, N defodiens had significantly greater pronotal width compared to conspecifics at each of the other eight sites. We hypothesize that the larger body size at this site which is dominated by N. defodiens may be selected because of the greater frequency of intraspecific encounters

Hours Restrictions and Labor Supply

This study presents a model of labor supply in which individuals may face constraints on their choice of work hours, and analyzes the sensitivity of parameter estimates and policy conclusions to the usual assumption of unrestricted choice. We set up the labor supply decision asa discrete choice problem, where each worker faces a finite number of employment opportunities, each offering fixed hours of work.The distribution from which these are drawn, as well as the number of draws, is estimated along with the behavioral parameters of individual labor supply.The standard model with unconstrained hours appears as a special case where the number of draws approaches infinity. We estimate the mean absolute difference between desired and actual work hours to be about ten hours perweek. The results strongly support the notion that hours choices are constrained, and suggest that models which ignore restrictions on hours worked may yield biased estimates of the wage elasticity of desired hours. Further, we suggest that analysis of policies such as income transfers and the flat rate tax which do not consider their effects on the distribution of hours offered may be very misleading.

Performance of a small, graphite electrode, multistage depressed collector with a 500-W, continuous wave, 4.8- to 9.6-GHz traveling wave tube

A small, isotropic graphite multistage depressed collector (MDC) and a short permanent magnet refocuser were designed, fabricated, and evaluated in conjunction with a 500-W, continuous-wave (CW), 4.8 to 9.6 GHz traveling wave tube (TWT). A novel performance optimization system and technique were used to optimize the TWT-MDC performance for saturated broad-band operation. The MDC performance was evaluated in both four- and three-stage configurations. Average TWT overall and four-stage collector efficiencies of 43.8 and 82.6 percent, respectively, were obtained for saturated octave-bandwidth operation. The isotropic graphite electrode material performed well, and shows considerable promise. However, considerably more test experience is required before definitive conclusions on its suitability for space and airborne TWT's can be made

Reducing the rare event: lessons from the implementation of a ventilator bundle.

The ventilator-associated event (VAE) is a potentially avoidable complication of mechanical ventilation (MV) associated with poor outcomes. Although rare, VAEs and other nosocomial events are frequently targeted for quality improvement efforts consistent with the creed to 'do no harm'. In October 2016, VA Greater Los Angeles (GLA) was in the lowest-performing decile of VA medical centres on a composite measure of quality, owing to GLA's relatively high VAE rate. To decrease VAEs, we sought to reduce average MV duration of patients with acute respiratory failure to less than 3 days by 1 July 2017. In our first intervention (period 1), intensive care unit (ICU) attending physicians trained residents to use an existing ventilator bundle order set; in our second intervention (period 2), we updated the order set to streamline order entry and incorporate new nurse-driven and respiratory therapist (RT)-driven spontaneous awakening trial (SAT) and spontaneous breathing trial (SBT) protocols. In period 1, the proportion of eligible patients with SAT and SBT orders increased from 29.9% and 51.2% to 67.4% and 72.6%, respectively, with sustained improvements through December 2017. Mean MV duration decreased from 7.2 days at baseline to 5.5 days in period 1 and 4.7 days in period 2; statistical process control charts revealed no significant differences, but the difference between baseline and period 2 MV duration was statistically significant at p=0.049. Bedside audits showed RTs consistently performed indicated SBTs, but there were missed opportunities for SATs due to ICU staff concerns about the SAT protocol. The rarity of VAEs, small population of ventilated patients and infrequent use of sedative infusions at GLA may have decreased the opportunity to achieve staff acceptance and use of the SAT protocol. Quality improvement teams should consider frequency of targeted outcomes when planning interventions; rare events pose challenges in implementation and evaluation of change

CRISPR/Cas9-based editing of a sensitive transcriptional regulatory element to achieve cell type-specific knockdown of the NEMO scaffold protein

The use of alternative promoters for the cell type-specific expression of a given mRNA/protein is a common cell strategy. NEMO is a scaffold protein required for canonical NF-κB signaling. Transcription of the NEMO gene is primarily controlled by two promoters: one (promoter B) drives NEMO transcription in most cell types and the second (promoter D) is largely responsible for NEMO transcription in liver cells. Herein, we have used a CRISPR/Cas9-based approach to disrupt a core sequence element of promoter B, and this genetic editing essentially eliminates expression of NEMO mRNA and protein in 293T human kidney cells. By cell subcloning, we have isolated targeted 293T cell lines that express no detectable NEMO protein, have defined genomic alterations at promoter B, and do not support activation of canonical NF-κB signaling in response to treatment with tumor necrosis factor. Nevertheless, noncanonical NF-κB signaling is intact in these NEMO-deficient cells. Expression of ectopic wildtype NEMO, but not certain human NEMO disease mutants, in the edited cells restores downstream NF-κB signaling in response to tumor necrosis factor. Targeting of the promoter B element does not substantially reduce NEMO expression (from promoter D) in the human SNU423 liver cancer cell line. Thus, we have created a strategy for selectively eliminating cell typespecific expression from an alternative promoter and have generated 293T cell lines with a functional knockout of NEMO. The implications of these findings for further studies and for therapeutic approaches to target canonical NF-κB signaling are discussed.Published versio

Generalized q-Deformed Symplectic sp(4) Algebra for Multi-shell Applications

A multi-shell generalization of a fermion representation of the q-deformed compact symplectic sp_q(4) algebra is introduced. An analytic form for the action of two or more generators of the Sp_q(4) symmetry on the basis states is determined and the result used to derive formulae for the overlap between number preserving states as well as for matrix elements of a model Hamiltonian. A second-order operator in the generators of Sp_q(4) is identified that is diagonal in the basis set and that reduces to the Casimir invariant of the sp(4) algebra in the non-deformed limit of the theory. The results can be used in nuclear structure applications to calculate beta-decay transition probabilities and to provide for a description of pairing and higher-order interactions in systems with nucleons occupying more than a single-j orbital.Comment: 10 page

An International Urogynecological Association (IUGA)/International Continence Society (ICS) joint report on the terminology for the conservative and nonpharmacological management of female pelvic floor dysfunction

There has been an increasing need for the terminology on the conservative management of female pelvic floor dysfunction to be collated in a clinically based consensus report.This Report combines the input of members and elected nominees of the Standardization and Terminology Committees of two International Organizations, the International Urogynecological Association (IUGA) and the International Continence Society (ICS), assisted at intervals by many external referees. An extensive process of nine rounds of internal and external review was developed to exhaustively examine each definition, with decision-making by collective opinion (consensus). Before opening up for comments on the webpages of ICS and IUGA, five experts from physiotherapy, neurology, urology, urogynecology, and nursing were invited to comment on the paper.A Terminology Report on the conservative management of female pelvic floor dysfunction, encompassing over 200 separate definitions, has been developed. It is clinically based, with the most common symptoms, signs, assessments, diagnoses, and treatments defined. Clarity and ease of use have been key aims to make it interpretable by practitioners and trainees in all the different specialty groups involved in female pelvic floor dysfunction. Ongoing review is not only anticipated, but will be required to keep the document updated and as widely acceptable as possible.A consensus-based terminology report for the conservative management of female pelvic floor dysfunction has been produced, aimed at being a significant aid to clinical practice and a stimulus for research

Remediasi Pemahaman Konsep Suhu dan Kalor Menggunakan Model Conceptual Understanding Procedures pada Siswa SMA

The purpose of this study is to determine the effect of remedy using Conceptual Understanding Procedures (CUPs) learning model to the students' concept understanding of temperature and heat in class X SMA Negeri 8 Pontianak. Method of this research is pre-Experimental with One-Group Pretest-Posttest Design which involves 32 students as the sample are selected by using intact group technique. Data collecting tool is multiple choice test with 14 items in opened ground. The result of data analysis show that the improvement of students' concept understanding after being given remedy is 27,23%. Wilcoxon test results, it is Z-ob tained = -4.953 at the significance level of 5%. It indicates that there is an improvement of students' concept understanding after being given remedy by using model CUPs. The effectiveness of remedy by using CUPs learning model is calculated with Cohen's Effect in order to obtain the effect size of 0.64 that is classified as moderate. The results of this study are expected to be an alternative to improve the students' concept understanding in other learning physic materials