Antioxidant and Anti-Inflammatory Potential of Cymbopogon nardus Ethanol Extract on 3T3-L1 Cells

Enny Rohmawaty,1 Hesti Lina Wiraswati,1 Tamara Aliya Zahra,2 Shabrina Nur Amalina,2 Julia Ramadhanti,1 Aziiz Mardanarian Rosdianto,1 Amila Laelalugina,3 Gita Tiara Dewi Nasution,1 Yusof Kamisah4 1Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Bandung, 40161, Indonesia; 2Undergraduate Program Medical Doctor, Faculty of Medicine, Universitas Padjadjaran, Bandung, 40161, Indonesia; 3Oncology and Stem Cell Working Group, Universitas Padjadjaran, Bandung, 40161, Indonesia; 4Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, 53000, MalaysiaCorrespondence: Enny Rohmawaty, Department of Biomedical Sciences, Faculty of Medicine, Universitas Padjadjaran, Jl. Ir. Soekarno KM. 21, Jatinangor, Sumedang, West Java, 45363, Indonesia, Email [email protected]: Cymbopogon nardus (L). Rendle has traditionally been recognized for its medicinal properties. Recent studies have suggested that its bioactive constituents possess antioxidant and anti-inflammatory properties. However, there is limited scientific evidence of its cellular effects. Given that the pathogenesis of many diseases involves oxidative stress and inflammation, this study aimed to evaluate the potential antioxidant and anti-inflammatory effects of the plant extracts in 3T3-L1 cells.Methods: Phytochemical screening of C. nardus extracts was performed to identify bioactive compounds. Antioxidant activity of the extract was assessed using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide dismutase (SOD) assays. Toxicity was evaluated using the MTT assay. Additionally, the effects of the extract on the gene expression of hypoxia-inducible factor 1α (HIF-1α) in menadione-induced 3T3-L1 cells, as well as interleukin-6 (IL-6) and cyclooxygenase-2 (COX-2) in lipopolysaccharide (LPS)-induced 3T3-L1 cells, were investigated.Results: Phytochemical screening revealed the presence of phenolics, tannins, alkaloids, and flavonoids in the ethanolic extracts. The extract demonstrated antioxidant activity, with IC50 values of 178.06 ppm for DPPH and 220 ppm for SOD. It did not affect the viability of 3T3-L1 cells at concentrations of up to 500 ppm. At 100 ppm, the extract increased cell viability (p< 0.05) and reduced HIF-1α expression in the menadione-treated cells (p< 0.05). Additionally, it decreased the expression of IL-6 and COX-2 in LPS-induced cells (p< 0.05).Conclusion: The ethanol extract of C. nardus demonstrated promising potential as an antioxidant and anti-inflammatory agent in 3T3-L1 cells. Further analysis is recommended to confirm the potential.Keywords: ROS, IL-6, COX-2, HIF-1α, menadione, lipopolysaccharid

THE INFLUENCE OF INVESTOR BEHAVIOR ON INVESTMENT DECISIONS WITH PROFIT INFORMATION AS A MODERATING VARIABLE

Abstract This research is quantitative research with an exploratory approach related to Investor Behavior, Investment Decisions, and Information Benefits. The data used in this research is of course primary data which is distributed online through a questionnaire containing 5 statement items for 14 questions covering 6 question items for the Investor Behavior variable, 4 question items for the Decision Variable variable, and 4 question items for the Information Profit variable. Researchers analyzed these data using the smart PLS 4.0 analysis tool with the hypothesis below. The result int this research show the Investor Behavior variable have a positive relationship and a significant influence on the Investment Decision variable can be accepted and even justified. This is because the P-Values results are positive and are below the significance level of 0.05, namely 0.002. These results are in line with research . The reason for creating these results, namely Investor Behavior, one of the characteristics is someone, in this case a student who is good at saving, is interested in investment, and has the desire to increase his finances many times over. Apart from this, the second hypothesis in this research can also be proven for the same reason, namely the P-Vlues value which has a positive relationship direction and is below the 0.05 significance level, namely 0.000. Thus the first and second hypotheses in this research can be proven and accepted. Keywords: Investor Behavior, Investor Decision, Profit Informatio

Cytological features of pap smear of multiparous women with mycoplasma hominis and ureaplasma urealyticum infection at outpatient clinic in medan

Abstract Mycoplasma hominis and Ureaplasma urealyticum were commensal pathogenic- potential bacterias in the female urogenital tract and may cause of newborns death. Several studies have suggested a correlation between cervical cytology and Mycoplasma genitalium and Ureaplasma urealyticum infections. It was a cross sectional design carried out on 50 multiparous women in an outpatient clinic in Medan in 2018. The cervical cytology features observed were reactive cellular change (RCC), cannonballs, predominance of coccoid bacteria, clue cells, and large number of neutrophils. All cytology features were negative from 3 (6.0%) subjects that had Mh infection. 9 (18.0%) subjects with Uu infection showed negative result of RCC, 9 (100.0%) were positive cannonballs, 3 (33.3%) were predominance of coccoid bacteria, 1 (11.1%) were positive clue cells and 7 (77.8%) had large number of neutrophils. Cytology finding of 38 (76.6%) subjects with no infection of Mh and Uu revealed 10 (26.3%) had RCC, 28 (73.7%) were positive cannonballs, 19 (50.0%) were predominance of coccoid bacteria, 2 (5.3%) were positive clue cells and 16 (42.1%) had large number of neutrophils. Non-specific cytological features were found in Mycoplasma infection. Specific features found in Ureaplasma infection but need to be traced to the infection of other organisms in group non Mycoplasma and Ureaplasma infections.</jats:p

PENGUATAN ORIENTASI KEWIRAUSAHAAN MASYARAKAT PENGRAJIN KRUPUK IKAN DI DESA BATAKAN, KECAMATAN PENYIPATAN, KABUPATEN TANAH LAUT

Kegiatan pengabdian kepada masyarakat ini bertujuan dalam rangka melaksanakan salah satu tridharma perguruan tinggi. Kegiatan ini mengambil objek kelompok usaha yang ada di desa dengan melibatkan masyarakat sekitar agar terjadi interaksi dan kolaborasi yang baik. Selain itu juga dengan melakukan belajar bersama lewat metode penyuluhan, FGD dan praktek bersama diharapkan akan dapat mengembangkan potensi kewirausahaan yang sudah ada dan dimiliki sebagian masyarakat. Selain itu bagi anggota masyarakat yang belum terbuka pikiran dan mindsetnya diharapkan akan dapat menumbuhkan dan mengembangkan potensi yang ada di dalam diri. Bagi anggota masyarakat yang sudah memiliki potensi berwirausaha diharapkan akan dapat memperkuat kemampuan berwirausahanya yang sudah ada selama ini. Melalui orientasi kewirausahaan yang terbangun dan berkembang di lingkungan rumah tangga dan makin meluas ke lingkup desa dan lainnya diharapkan akan dapat meningkatkan kesejahteraan masyarakat yang lebih besar lewat kemampuan menggali potensi diri dan desa/lingkungan dalam merubah dan menambah nilai social serta nilai ekonomi yang dapat dihasilkan.</jats:p