Protocatechuate 3, 4-Dioxygenase from Pseudomonas sp. : Partial Purification and Some Properties

Article信州大学農学部紀要 23(1): 43-49(1986)departmental bulletin pape

Activities of Angiotensin-I Converting Enzyme Inhibition in Proteolytic Hydrolyzate of Food Proteins: In View of Development of Physiologically Functional Peptides

ACE inhibitory activity in hydrolyzate of food proteins treated by some proteases was tested. The protein from soybean, when it was hydrolyzed by proteases, had larger ACE inhibitory activity, especially at neutral pH. At lower and higher pH all hydrolyzate did not show high ACE inhibitory activity contrary to our expectations. Without treatment of protease, on the contrary, soybean protein had lower ACE inhibitory activity than those of wheat and corn.Article信州大学農学部紀要 26(1・2): 13-20(1990)departmental bulletin pape

Activity of Naringinase in the Extracts from Citrus unshiu waste Treated with Brewer's Yeasts and Their Inhibitory Effects on Angiotensin Converting Enzyme

Naringinase activity and inhibitory potency against Angiotensin- converting enzyme were tested in the extract from Citrus unshiu waste treated with various brewer's yeasts. Naringinase activity in the yeasts tested was much smaller than that in some fungi. It was, however, proved that if an appropriate incubation method is adopted, analysis method is effective for this system. Inhibitory effect in the extract, on the contrary, was shown being positive in almost all strains tested. These results suggest that this approach will be a promising method to make a component of functional foods.Article信州大学農学部紀要 25(1): 37-44(1988)departmental bulletin pape

The Influence of Mixing Conditions of Reaction Medium on Protease-Catalyzed Peptide Synthesis: The Use of Sonication

Sonication was demonstrated to be a useful method mixing reactoin medium. A model reaction, Boc-Gly-OH + H-Phe-N₂H₂Ph → Boc-Gly-phe-N₂H₂Ph, was adopted to compare two mixing methods of medium, sonication and magnetic-stirring. Under biphasic system including 25% of organic solvent, sonication was preferable to magnetic-stirring for several cosolvents and showed being able to have some potentiality as mixing method. On the other hand another model reaction, Boc-Tyr (Bzl)-OH + H-Gly-N₂H₂Ph→Boc-Tyr (Bzl)-Gly-N₂H₂Ph, which had not been achieved by other investigators, was progressed by the new mixing method. This new method may form other certain reaction-field in the medium.Article信州大学農学部紀要 26(1・2): 21-26(1990)departmental bulletin pape

Protocatechuate 3, 4-Dioxygenase from Pseudomonas sp. : Partial Purification and Some Properties

Articleapplication/pdf信州大学農学部紀要 23(1): 43-49(1986)departmental bulletin pape