Cryo-EM map interpretation and protein model-building using iterative map segmentation.

A procedure for building protein chains into maps produced by single-particle electron cryo-microscopy (cryo-EM) is described. The procedure is similar to the way an experienced structural biologist might analyze a map, focusing first on secondary structure elements such as helices and sheets, then varying the contour level to identify connections between these elements. Since the high density in a map typically follows the main-chain of the protein, the main-chain connection between secondary structure elements can often be identified as the unbranched path between them with the highest minimum value along the path. This chain-tracing procedure is then combined with finding side-chain positions based on the presence of density extending away from the main path of the chain, allowing generation of a Cα model. The Cα model is converted to an all-atom model and is refined against the map. We show that this procedure is as effective as other existing methods for interpretation of cryo-EM maps and that it is considerably faster and produces models with fewer chain breaks than our previous methods that were based on approaches developed for crystallographic maps

Attachment and amae in Japanese romantic relationships

This is the post-print version for the Article. The official published version can be accessed from the link below - Copyright @ 2012 John Wiley & SonsAmae is a Japanese term that refers to an individual’s inappropriate behavior when he/she presumes indulgence from a significant other. The link between attachment style and amae has been debated, but few studies have examined this link empirically. This study examined the association of attachment style with amae behavior in Japanese dating couples over a two-week period. Results showed that for Japanese men, anxious attachment was positively associated with their amae behavior, and in turn, with their increased relationship quality. Conversely, avoidant attachment was negatively associated with their amae behavior, and in turn, with their decreased relationship quality.This research was supported by a Postdoctoral Fellowship grant awarded by the Japanese Society for the Promotion of Science to the author

Cognitive distortions as social practices: An examination of cognitive distortions in sex offender treatment froma discursive psychology perpective

This article presents a critique of the concept of cognitive distortion as it has been developed within the domain of sex-offender treatment programme theory and practice. Drawing upon a discursive psychology perspective, it is argued that cognitive distortions should not be considered as mental entities but as social practices. This argument is illustrated by closely examining how offenders’ accounts of their offences during sex offender treatment sessions were organised. Recordings and transcriptions of treatment group sessions were analysed for the occurrence of regular patterns of talk and interaction. This analysis focused on how minimisation was achieved through well documented rhetorical and conversational devices (conversational repair, narrative contrast devices). An orientation to cognitive distortions as a resource was also illustrated through examining its use by group members to admonish a focus offender and through a narrative reflexivity device. These findings suggest that the notion of cognitive distortion and its role in treatment settings should be reconsidered. Furthermore, it is suggested that a discursive psychology perspective can also make a highly relevant contribution to the evaluation of treatment group processes and that further research is needed in order to examine in detail the way that treatment groups are socially organised

Towards a New Framework for Product Development

In the mid-1980s, Andreasen and Hein first described their model of Integrated Product Development. Many Danish companies quickly embraced the principles of integrated product development and adapted the model to their specific business and product context. However, there is concern amongst many Danish companies that Integrated Product Development no longer provides a sufficient way of describing industry’s product development activity. More specifically, five of these companies have supported a programme of research activities at the Technical University of Denmark, which seeks to develop a new framework for product development. This paper will describe the research approach being taken, present some initial findings, and outline a vision of a new working approach to product development

Protecting the flight test programme

Considerable effort is put into the safety risk assessment of any flight test programme - and rightly so, since failures to apply best practice in this area may cause significant expense, and in extremis loss of life. However, it must also be remembered that the flight test programme itself is an essential component in the development of the aircraft or system, and that even if safety is never compromised, the failure of a test programme to deliver the required results on time and budget can cause failure of the entire aircraft programme. This paper considers the areas in which planning and conduct of a flight test programme should be protected. In particular it considers the conduct of flight test personnel in ways which go beyond only safety training, the important of documenting all flight test planning and conduct and the continuous justification of flight test conclusions, planning project manning to ensure that the loss (for whatever reason) of key personnel or equipment does not cause complete failure of the flight test programme, how to recover from significant programme disruptions, and most importantly whilst protecting or recovering the flight test programme – how to ensure that safety is not compromised in the process

Increasing Distributed Generation Penetration using Soft Normally-Open Points

This paper considers the effects of various voltage control solutions on facilitating an increase in allowable levels of distributed generation installation before voltage violations occur. In particular, the voltage control solution that is focused on is the implementation of `soft' normally-open points (SNOPs), a term which refers to power electronic devices installed in place of a normally-open point in a medium-voltage distribution network which allows for control of real and reactive power flows between each end point of its installation sites. While other benefits of SNOP installation are discussed, the intent of this paper is to determine whether SNOPs are a viable alternative to other voltage control strategies for this particular application. As such, the SNOPs ability to affect the voltage profile along feeders within a distribution system is focused on with other voltage control options used for comparative purposes. Results from studies on multiple network models with varying topologies are presented and a case study which considers economic benefits of increasing feasible DG penetration is also given

The role of customer experience in building brand loyalty within the service context

Customer experience theory, research, and practice represent an evolving area of study within the marketing discipline. Despite its importance, the customer experience concept remains vague and lacks a thorough theoretical foundation. This study addresses this gap in the literature and examines the antecedents and consequences of customer experience from customer perspectives. The study provides a conceptual framework building from a qualitative study and the existing literature. This article includes a formal test of the framework using a large-scale survey of British customers to examine their experience with resort-hotel brands. The results show price perception, core services, and word-of-mouth have a direct impact on how customers interpret their experiences with resort-hotel brands; perceived service quality plays a mediatory role in the relationship between servicescape, core service, and customer experience. Measuring validation strength of customer experience upon brand loyalty by best fit in combination with cross-sample predictive validity models is a valuable contribution of this study

A structural model to examine the antecedents and consequences of customer with experiential brands

Despite the increasing attention towards customer experience, empirical research to explain this notion, its antecedents and its consequences is still limited. Hence, this research aims to develop a framework of customer experience based on a number of antecedents that influence customer experience in the service sector. This paper presents the first part of the study which includes the review of literature, the application of netnography method to the development of customer experience construct, the development of the research measures and the hypotheses development for the structural model. The second part of the study, which is presented in another paper, provides the results of the empirical study and discusses the findings of the structural model

Was Schön ever right?

A few years ago, Jan Hendrik Schön impressed the world of science with exciting ideas and amazing experiments. Those experiments are believed no more. But were we wrong to admire the ideas at the time, or have we been too ready to turn our backs on novel ideas because they are associated with misconduct

Maximum Effectiveness of Electrostatic Energy Harvesters When Coupled to Interface Circuits

Accepted versio