Seipin and the membrane-shaping protein Pex30 cooperate in organelle budding from the endoplasmic reticulum

Lipid droplets (LDs) and peroxisomes are ubiquitous organelles with central roles in eukaryotic cells. Although the mechanisms involved in biogenesis of these organelles remain elusive, both seem to require the endoplasmic reticulum (ER). Here we show that in yeast the ER budding of these structurally unrelated organelles has remarkably similar requirements and involves cooperation between Pex30 and the seipin complex. In the absence of these components, budding of both LDs and peroxisomes is inhibited, leading to the ER accumulation of their respective constituent molecules, such as triacylglycerols and peroxisomal membrane proteins, whereas COPII vesicle formation remains unaffected. This phenotype can be reversed by remodeling ER phospholipid composition highlighting a key function of these lipids in organelle biogenesis. We propose that seipin and Pex30 act in concert to organize membrane domains permissive for organelle budding, and that may have a lipid composition distinct from the bulk ER

Membrane dynamics and organelle biogenesis—lipid pipelines and vesicular carriers

Discoveries spanning several decades have pointed to vital membrane lipid trafficking pathways involving both vesicular and non-vesicular carriers. But the relative contributions for distinct membrane delivery pathways in cell growth and organelle biogenesis continue to be a puzzle. This is because lipids flow from many sources and across many paths via transport vesicles, non-vesicular transfer proteins, and dynamic interactions between organelles at membrane contact sites. This forum presents our latest understanding, appreciation, and queries regarding the lipid transport mechanisms necessary to drive membrane expansion during organelle biogenesis and cell growth

Search for heavy Majorana neutrinos in e^±e^±+ jets and e^±µ^±+ jets events in proton-proton collisions at √s = 8 TeV

A search is performed for heavy Majorana neutrinos (N) decaying into a W boson and a lepton using the CMS detector at the Large Hadron Collider. A signature of two jets and either two same sign electrons or a same sign electron-muon pair is searched for using 19.7 fb-1 of data collected during 2012 in proton-proton collisions at a centre-of-mass energy of 8TeV. The data are found to be consistent with the expected standard model (SM) background and, in the context of a Type-1 seesaw mechanism, upper limits are set on the cross section times branching fraction for production of heavy Majorana neutrinos in the mass range between 40 and 500 GeV. The results are additionally interpreted as limits on the mixing between the heavy Majorana neutrinos and the SM neutrinos. In the mass range considered, the upper limits range between 0.00015-0.72 for |VeN|2 and 6.6×10-5-0.47 for |VeNV * µN|2/(|VeN|2+|VµN|2), where V ℓN is the mixing element describing the mixing of the heavy neutrino with the SM neutrino of flavour ℓ. These limits are the most restrictive direct limits for heavy Majorana neutrino masses above 200 GeV.we acknowledge the enduring support for the construction and operation of the LHC and the CMS detector provided by the following funding agencies: BMWFW and FWF (Austria); FNRS and FWO (Belgium); CNPq, CAPES, FAPERJ, and FAPESP (Brazil); MES (Bulgaria); CERN; CAS, MoST, and NSFC (China); COLCIENCIAS (Colombia); MSES and CSF (Croatia); RPF (Cyprus); MoER, ERC IUT and ERDF (Estonia); Academy of Finland, MEC, and HIP (Finland); CEA and CNRS/IN2P3 (France); BMBF, DFG, and HGF (Germany); GSRT (Greece); OTKA and NIH (Hungary); DAE and DST (India); IPM (Iran); SFI (Ireland); INFN (Italy); MSIP and NRF (Republic of Korea); LAS (Lithuania); MOE and UM (Malaysia); CINVESTAV, CONACYT, SEP, and UASLP-FAI (Mexico); MBIE (New Zealand); PAEC (Pakistan); MSHE and NSC (Poland); FCT (Portugal); JINR (Dubna); MON, RosAtom, RAS and RFBR (Russia); MESTD (Serbia); SEIDI and CPAN (Spain); Swiss Funding Agencies (Switzerland); MST (Taipei); ThEPCenter, IPST, STAR and NSTDA (Thailand); TUBITAK and TAEK (Turkey); NASU and SFFR (Ukraine); STFC (United Kingdom); DOE and NSF (USA)

Search for heavy Majorana neutrinos in e(+/-)e(+/-)+ jets and e(+/-) mu(+/-)+jetseventsinproton-protoncollisionsat root s=8TeV

A search is performed for heavy Majorana neutrinos (N) decaying into a W boson and a lepton using the CMS detector at the Large Hadron Collider. A signature of two jets and either two same sign electrons or a same sign electron-muon pair is searched for using 19.7 fb−1 of data collected during 2012 in proton-proton collisions at a centre-of-mass energy of 8 TeV. The data are found to be consistent with the expected standard model (SM) background and, in the context of a Type-1 seesaw mechanism, upper limits are set on the cross section times branching fraction for production of heavy Majorana neutrinos in the mass range between 40 and 500 GeV. The results are additionally interpreted as limits on the mixing between the heavy Majorana neutrinos and the SM neutrinos. In the mass range considered, the upper limits range between 0.00015–0.72 for |VeN| 2 and 6.6×10−5–0.47 for |VeNV ∗ µN | 2/(|VeN| 2 +|VµN | 2 ), where V`N is the mixing element describing the mixing of the heavy neutrino with the SM neutrino of flavour `. These limits are the most restrictive direct limits for heavy Majorana neutrino masses above 200 GeV