A New Outbreak in Sea Bass Farming in Turkey: Aeromonas veronii

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗTurkey produces most of the European sea bassin Europe and bacterial diseases are the main cause of economic loss during the production cycle. This research presents the first extended study of the Aeromonas veronii infection in sea bass on the Turkish coast of the Aegean Sea. An outbreak was observed in three different sea bass farms and diseased fish with clinical symptoms were sampled. Abdominal distention, hemorrhages on the body and anus, enlarged spleen and liver, and hemorrhages in the internal organs were detected from clinical and pathological examinations. Biochemical and molecular identification confirmed the pathogen to be A.veronii.The histopathological observations demonstrated that the pathogen caused bacterial colonies in the fibrous connective tissue, granuloma, and vacuolar degeneration.The primary causes of the disease were proved through an infection experiment. 80% and 90% mortality were calculated in 0.85 x 108 CFU ml-1 and 1.28 x 108 CFU ml-1 experimentally infected groups with clinical and pathological signs of the disease respectively. Recently, the pathological symptoms of the disease had been confused with pasteurellosis infection in cage farms but the presence of A.veronii has been confirmed in the current study. A detailed study is needed to investigate the overall status of the disease in the Aegean Sea in order to design an appropriate preventive strategy

First Report of Saprolegnia parasitica from a Marine Species: Gilthead Seabream (Sparus aurata) in Brackish Water Conditions

ΔΕΝ ΔΙΑΤΙΘΕΤΑΙ ΠΕΡΙΛΗΨΗSaprolegniosis is a serious fungal disease that mostly affects freshwater fish species and eggs. It has a cotton wool-like appearance on the body of amphibians, crustaceans and several fish species. Infected gilthead seabream (Sparus aurata) were subjected to clinical, microbiological, parasitological and pathological investigation. On the infected skin samples, grey-white cotton-like patches, erosion of the skin and scale affusion were detected. Lesions covered the whole body of S. aurata in the advanced stages. Bacterial growth and parasitic symptoms were not observed in microbiological examination. Microscopic examination showed hyphaes carrying cysts that were long and branched. In scanning electron microscopy overviews fungal zoospores were observed. In histopathological observations of sections of skin, erosive-ulcerative dermatitis and mycelium of Saprolegnia parasitica were seen in the muscle tissue. Gene sequence-based identification found Saprolegnia parasitica. S. parasitica has not until now been detected in S.aurata. The low salinity of the brackish water is believed to be the predisposing factor of Saprolegniosis in sea bream in this case

Mix Infections in Rainbow Trout (Oncorhynchus mykiss Walbaum): Lactococcus garvieae and Vibrio anguillarum O1

Vibrio anguillarum and Lactococcus garvieae which causes mixed infections were isolated for the first time from rainbow trout farms in the Southwest of Turkey. The outbreak occurred at 15 degrees C water temperature in June 2009. The diseased fish showed erratic swimming, dark discoloration, bilateral exopthalmia, haemorhages on the fins, operculum, the liver, spleen and lower intestines with mortality ranging from 30-50% in the pools. The identities of the isolates were confirmed as V. anguillarum O1 and L. garvieae by biochemical and serological tests using API 20 Strep, API 20 E and API Strept system test strips and also V. anguillarum (ATTC 43305) used as antigens for agglutination. The epizootic disease was effectively controlled by oral administration of enrofloxacin

Efficacy of a whole cell Lactococcus garvieae vaccine in rainbow trout (Oncorhynchus tnykiss)

In this research, a whole cell bacterin vaccine was prepared from Lactococcus garvieae isolated from rainbow trout farms in Turkey after an epizootic outbreak. The efficacy of vaccine on lactococcosis was tested in rainbow trout (Oncorhynchus myciss). In 25, 76 and 125 days following i.p. injection vaccination, the groups of vaccinates and non vaccinates were challenged by i.p. injection with 0.1 mL of the Lactococcus garvieae strain at concentration of approximately 10 6 cells mL -1 and monitored daily for clinical signs and mortality for 21 days. Dead fish were examined to confirm the reisolation of the inoculated strain from the internal organs. Calculated RPS values for day 25, 76 and 125 were found 83.3, 75 and 68%, respectively. © Medwell Journals, 2012

Mix infections in rainbow trout (Oncorhynchus mykiss Walbauni): Lactococcus garvieae and Vibrio anguillarum O1

Vibrio anguillarum and Lactococcus garvieae which causes mixed infections were isolated for the first time from rainbow trout farms in the Southwest of Turkey. The outbreak occurred at 15°C water temperature in June 2009. The diseased fish showed erratic swimming, dark discoloration, bilateral exopthalmia, haemorhages on the fins, operculum, the liver, spleen and lower intestines with mortality ranging from 30-50% in the pools. The identities of the isolates were confirmed as V. anguillarum O1 and L. garvieae by biochemical and serological tests using API 20 Strep, API 20 E and API Strept system test strips and also V. anguillarum (ATTC 43305) used as antigens for agglutination. The epizootic disease was effectively controlled by oral administration of enrofloxacin. © Medwell Journals, 2011