Subtle balance of tropoelastin molecular shape and flexibility regulates dynamics and hierarchical assembly

The assembly of the tropoelastin monomer into elastin is vital for conferring elasticity on blood vessels, skin, and lungs. Tropoelastin has dual needs for flexibility and structure in self-assembly. We explore the structure-dynamics-function interplay, consider the duality of molecular order and disorder, and identify equally significant functional contributions by local and global structures. To study these organizational stratifications, we perturb a key hinge region by expressing an exon that is universally spliced out in human tropoelastins. We find a herniated nanostructure with a displaced C terminus and explain by molecular modeling that flexible helices are replaced with substantial β sheets. We see atypical higher-order cross-linking and inefficient assembly into discontinuous, thick elastic fibers. We explain this dysfunction by correlating local and global structural effects with changes in the molecule’s assembly dynamics. This work has general implications for our understanding of elastomeric proteins, which balance disordered regions with defined structural modules at multiple scales for functional assembly.United States. Office of Naval Research (Presidential Early Career Award for Scientists and Engineers)National Institutes of Health (U.S.) (U01 EB014976

Unraveling the molecular mechanisms of thermo-responsive properties of silk-elastin-like proteins by integrating multiscale modeling and experiment

Adaptive hydrogels tailor-made from silk-elastin-like proteins (SELPs) possess excellent biocompatibility and biodegradability with properties that are tunable and responsive to multiple simultaneous external stimuli. To unravel the molecular mechanisms of their physical response to external stimuli in tandem with experiments, here we predict and measure the variation in structural properties as a function of temperature through coarse-grained (CG) modeling of individual and crosslinked SE8Y and S4E8Y molecules, which have ratios of 1:8 and 4:8 of silk to elastin blocks respectively. Extensive structural reshuffling in single SE8Y molecules led to the increased compactness of the structure, whereas S4E8Y molecules did not experience any significant changes as they already adopted very compact structures at low temperatures. Crosslinking of SE8Y molecules at high concentrations impeded their structural transition at high temperatures that drastically reduced the degree of deswelling through extensive suppression of the structural shuffling and the trapping of the molecules in high potential energy states due to inter-molecular constraints. This integrative experimental and computational understanding of the thermal response in single molecules of SELPs and their crosslinked networks should lead to further improvements in the properties of SELP hydrogels through predictive designs and their wider applications in biomaterials and tissue engineering.United States. Department of Defense. Office of Naval Research (Grant N00014-16-1-233)United States. National Institutes of Health (Grant U01 EB014976)Singapore. Agency for Science, Technology and Research (Grant A1786a0031)United States. National Science Foundation. (Grant ACI-1053575

SPACs: Financial IPO Tool Gone Wrong – Awaiting SEC Recourse: A Study on the Resurgence of SPACs Following the M2 Money Supply Stimulus by the Federal Reserve in March 2020

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Приоритеты России во взаимодействии со странами Шанхайской организации сотрудничества

In the article the basic directions of activity of the Russian Federation in the framework of the Shanghai cooperation organization are considered. The conclusion is that Russia from the moment of foundation of the Organization actively supports association of the member countries’ positions of the Shanghai cooperation organization in the sphere of ensuring regional security. The analysis was carried out of economic policy concerning Organization member countries. As a result revealed that the creation of energy club lobbied by the Russian Federation shows the paramount importance of the energy component of the countries cooperation of the Organization. Highlight some positions that determine the importance of Russian energy diplomacy in the activities of the Shanghai cooperation organization

LEGAL AND ETHICAL ASPECTS OF HOSPITALIZATION INCAPACITATED PATIENTS TO PSYCHIATRIC HOSPITAL

Тhe article examines legal and ethical issues of admission of incapacitated patients to specialized psychiatric hospital. Main legislative and normative documents, necessary for decision-making are described. Private clinical case was envisaged in this article

PROVIDING REGIONAL SECURITY WITHIN THE FRAMEWORK OF THE SHANGHAI COOPERATION ORGANIZATION

События последних лет в странах Северной Африки и Ближнего Востока («арабская весна»), способствовали актуализации вопросов безопасности в деятельности региональных и глобальных организаций, в частности Шанхайской организации сотрудничества (ШОС). Основным направлением деятельности ШОС является укрепление региональной безопасности. Региональная антитеррористическая структура Шанхайской организации сотрудничества (РАТС ШОС) координирует деятельность, связанную с обменом информацией и укреплением сотрудничества в борьбе с терроризмом, экстремизмом и сепаратизмом.Целью исследования является определение эффективности работы РАТС ШОС в обеспечении региональной безопасности.При исследовании использовались методы как теоретического, так и эмпирического уровня. Особенно полезным оказался системный подход, который позволил рассмотреть деятельность внутренних структур РАТС ШОС и мероприятий проводимых в рамках данного института ШОС в совокупности.В результате выявлено, что ШОС постепенно становится важнейшим инструментом в борьбе с экстремизмом и терроризмом в центральноазиатском регионе. За последние два года, в рамках ШОС была проведена большая серия совместных антитеррористических, военных и военно-морских учений. Важно подчеркнуть, что разработанная договорно-правовая база Организации дает возможность осуществлять антитеррористические мероприятия в рамках международно-правового поля.Материалы статьи могут использоваться для сравнительного анализа работы различных структур, межгосударственных союзов, деятельность которых направлена на борьбу с региональным и международным терроризмом.Recent events in North Africa and the Middle East (the “Arab Spring”) contributed to the mainstreaming of security issues in the regional and global organizations, such as the Shanghai Cooperation Organization (SCO). The main activity of the SCO is a strengthen regional security. The Regional anti-terrorist structure of Shanghai Cooperation Organization (RATS SCO) coordinates activity connected with the information exchange and strengthening cooperation in the struggle against terrorism, extremism and separatism.The objective of the study is to determine the effectiveness of the RATS SCO in regional security.Methods of research are theoretical and empirical level. A systematic approach was particularly useful. He allowed to review the activities of the internal structures of the RATS SCO and activities in the framework of the SCO in as a whole.The results showed that SCO is gradually becoming an important tool in the struggle against extremism and terrorism in the Central Asia region. A large series of joint anti-terrorism, military and navy trainings were conducted in the framework of the SCO. It is significant that developed international legal base of the Organization makes it possible to carry out anti-terrorist activities in the international legal field.The results can be used for comparative analysis of the various interstate structures, whose activities are aimed at struggle with regional and international terrorism

Экономическое сотрудничество Китайской Народной Республики со странами Шанхайской организации сотрудничества

The article analyzes the economic policy of People's Republic of China concerning members of the Shanghai cooperation organization. It is revealed that, Shanghai cooperation organization has taken a strong position in the political life of the region and especially in Central Asia. Under the aegis of the Shanghai cooperation organization is implementing a number of major projects that could drastically change the lives of the region. At the heart of these projects is an active work of China. People's Republic of China creates interstate union with Kazakhstan, Turkmenistan and Uzbekistan on the development of energy resources of these countries

Priorities of Russia in Cooperation with Countries of the Shanghai Cooperation Organization

In the article the basic directions of activity of the Russian Federation in the framework of the Shanghai cooperation organization are considered. The conclusion is that Russia from the moment of foundation of the Organization actively supports association of the member countries’ positions of the Shanghai cooperation organization in the sphere of ensuring regional security. The analysis was carried out of economic policy concerning Organization member countries. As a result revealed that the creation of energy club lobbied by the Russian Federation shows the paramount importance of the energy component of the countries cooperation of the Organization. Highlight some positions that determine the importance of Russian energy diplomacy in the activities of the Shanghai cooperation organization

COMPUTER ANALYSIS OF THE UNDERLYING EQUILIBRIUM MODELS WITH PUBLIC GOOD

This article discusses an option of the mathematical model of economic situations with public goods consumed by several market participants simultaneously. Computer analysis of this model was performed by using the MATHCAD package which allows toillustrate some techniques of determining of market balance. It was assumed that the participants, considering the choice of the other is equal to their previous decision, comes to the agreed result in finish

Economic Cooperation of the People’s Republic of China with the Countries of the Shanghai Cooperation Organization

The article analyzes the economic policy of People’s Republic of China concerning members of the Shanghai cooperation organization. It is revealed that, Shanghai cooperation organization has taken a strong position in the political life of the region and especially in Central Asia. Under the aegis of the Shanghai cooperation organization is implementing a number of major projects that could drastically change the lives of the region. At the heart of these projects is an active work of China. People’s Republic of China creates interstate union with Kazakhstan, Turkmenistan and Uzbekistan on the development of energy resources of these countries