Breast Cancer Migration Is Inhibited By Ficus Glomerata Roxb Leaf Column Elute Via An Inflammatory And Apoptotic Cell Signalling Pathway

Among developed countries, breast carcinoma is one of the most common cancers in women. Breast carcinoma is the result of the fusion of several biological structures, each with its own physiological characteristics and clinical repercussions. The Ficus genus has been reported to have important pharmacological properties in a variety of plants. The current study examined at the anti-mammary action of Ficus glomerata leaf column elute (FGLCE) in 12-Dimethylbenz (α) anthracene (DMBA) driven breast cancer and the mechanism behind it. To produce tumors in female rats, a single dosage of DMBA (25 mg/kg) was injected into the mammary gland. The female rats were divided into five groups- Group I. Control group; Group II- control + FGLCE (100mg/kg of BW); Group III- DMBA treated rats; Group IV- DMBA + FGLCE treated; Group V- DMBA + DOX (5 mg/kg) treated rats. Tumor burden, incidence, and volume were measured at the end of the experiment.The lipid profile (TC, FFA, PL, and TG) in plasma, liver tissue, and mammary tissue were all calculated.At a dose of 25 mg/kg body weight, DMBA inhibits the activity of antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), and glutathione peroxidase (GPX).DMBA  at a dose concentration 25mg/ kg body weight xenobiotic  markers increased in group III rats When compared to the control group  and glycoprotein p53, and TNF-α, the dose-dependent treatment with FGLCE (P<0.05) significantly reduced .Hepatic markers such as alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), tumor necrosis factor- (TNF-α), and inflammatory mediators such as nuclear factor kappa-B(NF-kB) were considerably (P<0.05) down regulated by FGLCE. FGLCE significantly (P<0.05) reduced apoptotic regulator (Bax), B-cell lymphoma 2 (Bcl-2), and Breast Cancer gene 1 (BRCA-1) expression when compared to DMBA control group rats.Therefore, the findings suggest that FGLCE has a protective effect against DMBA-induced mammary gland in rats

In-silico molecular docking analysis of some plant derived molecules for anti-inflammatory inhibitory activity

Herbs are essential resources for drug discovery. However, numerous challenges stand in front of the scientific community to discover novel drugs from herbs. To explore the validation behind the precious knowledge of traditional medicine, we focused on achieving virtual screening to detect the potential medicines from the herbs.  Five bioactive compounds from known anti-inflammatory medicinal plants were examined through molecular docking against  cyclooxygenase-2 (COX-2) and inducible Nitric Oxide Synthase (iNOS), using AutoDock 4.2. The docking of selected ligands with COX-2 showed the binding energy varying from -6.15 Kcal/mol to ‑11.24 Kcal/mol. The docking energies of identified ligands with iNOS were generated ranges from -3.85kcal/mol to -6.99 kcal/mol.  Among the tested ligands, it was noted that 6 urs-12-en-24-oic acid showed the best binding energy than other compounds with the lowest binding energy and highest binding affinity with both anti-inflammatory target proteins COX-2 and iNOS. The in silico study validates the potential phytochemical compound of the medicinal herb that contribute to anti-inflammatory activity with low toxicity and minimal side effects

Evaluation of surface water for drinking purpose with seasonality of water quality index

The present investigation was aimed to evaluate the surface water by analysing the physico-chemical parameters in Vaigai reservoir and Pickup dam from February, 2016 to March, 2018. Physical and chemical properties were analyzed using APHA methods and the result of 16 parameters was used for Water Quality Index analysis. It was high in Vaigai reservoir than in Pickup dam. Water of Vaigai reservoir was good except Southwest monsoon I and II, Northeast monsoon I and winter II. Pickup dam quality of water was poor only during Southwest monsoon II and II, Northeast monsoon II. It was good in quality for other seasons. Poor quality of water during monsoon needs care to prevent pollution.</jats:p

In-silico molecular docking analysis of some plant derived molecules for anti-inflammatory inhibitory activity

Herbs are essential resources for drug discovery. However, numerous challenges stand in front of the scientific community to discover novel drugs from herbs. To explore the validation behind the precious knowledge of traditional medicine, we focused on achieving virtual screening to detect the potential medicines from the herbs.  Five bioactive compounds from known anti-inflammatory medicinal plants were examined through molecular docking against  cyclooxygenase-2 (COX-2) and inducible Nitric Oxide Synthase (iNOS), using AutoDock 4.2. The docking of selected ligands with COX-2 showed the binding energy varying from -6.15 Kcal/mol to ‑11.24 Kcal/mol. The docking energies of identified ligands with iNOS were generated ranges from -3.85kcal/mol to -6.99 kcal/mol.  Among the tested ligands, it was noted that 6 urs-12-en-24-oic acid showed the best binding energy than other compounds with the lowest binding energy and highest binding affinity with both anti-inflammatory target proteins COX-2 and iNOS. The in silico study validates the potential phytochemical compound of the medicinal herb that contribute to anti-inflammatory activity with low toxicity and minimal side effects.</jats:p

Conversion of poultry litter into class A biosolids using autothermal thermophilic aerobic digestion

Purpose – The purpose of this paper is to minimize environmental problems related to raw poultry manure application by stabilizing its nutrient and organic matter (OM) content. This can be achieved by prior digestion before its application to agricultural soils. Design/methodology/approach – In the present study, changes in physicochemical and microbial properties of poultry litter were studied in order to evaluate the suitability of using the digested (stabilized) product for soil amendment. The poultry litter was digested in autothermal thermophilic aerobic digestor (ATAD) where organic solids were degraded and the heat released during the microbial degradation was used to maintain the thermophilic temperatures ( &lt; 50ºC) at a hydraulic retention time of about 3 d. Findings – Results of this study showed that the poultry litter undergoes physicochemical and microbial changes similar to other digestion systems; these changes include self-heating, relative increase in Cu, Zn, Fe, Mn, P, K and NO3-N and decrease in microbial population numbers, C, OM and NH4-N contents. Originality/value – ATAD is an effective method for the conversion of poultry litter into organic fertilizer, which can be readily applied to the agriculture land. ATAD is an eco-friendly, cost effective method which also reduces the length of stabilization. </jats:sec