Phosphate absorption by Arabidopsis thaliana : the effects of phosphorus nutritional status : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Plant Biology and Biotechnology at Massey University, Palmerston North, New Zealand

The effect of phosphorus nutritional status on phosphate uptake within the concentration range of the high affinity uptake mechanism, and subsequent translocation to the shoot was investigated in the plant species Arabidopsis thaliana. Plants of different nutritional status were generated by exposure to different set phosphate concentrations throughout an aseptic hydroponic growing period. Alternatively phosphorus deficiency was induced by growth at high concentrations of phosphate followed by a period of 5 days in phosphate-free hydroponic solution. In effect these growth conditions resulted in plants of distinguishable phenotypic character with respect to phosphate absorption, phosphate translocation, arsenate sensitivity and root-shoot ratio. To determine absorption kinetics nutrient depletion trials were carried out in which phosphate uptake was measured by monitoring the loss of phosphate from depletion solutions of set initial phosphate concentration to which the root systems of intact plants were exposed. Km and Vmax kinetic parameters were calculated from the depletion trial data using the software package "Igor Pro". Influx and net phosphate uptake was determined by setting the initial phosphate concentration of the depletion trials using either 32 P labelled KH 2 P0 4 or non-labelled KH 2 P0 4 respectively. Radioactivity was measured by counting the Cerenkov radiation in a scintillation counter. Non-labelled phosphate depletion was measured by either spectrophotometric assay or ion chromatography. To asses the effect of the phosphate analogue arsenate on phosphate influx, 32 P labelled phosphate uptake was measured with arsenate (KH 2 AsO 4 ) present in the depletion solution at a concentration of 20 µM. Phosphate translocation was determined by counting the Cerenkov radiation in the roots and shoots separately of plants that had been exposed to the 32 P labelled depletion solutions. Under the conditions of this project, phosphorus deficient plants exhibited alterations in the kinetic parameters Km and Vmax for phosphate uptake that were dependent on how the deficiency was induced. For plants that were grown continuously at low phosphate concentrations Km was decreased without a concomitant change in Vmax. For plants that were grown at high concentrations of phosphate followed by a 5 day period of phosphate starvation, a significant increase in Vmax was recorded without an associated change to Km. Phosphate uptake was found to be severely inhibited by the presence of arsenate in the depletion solution. Greatest inhibition however was found not to occur at the level of absorption into the plant root system but rather appeared to be at a site involved in phosphate loading into the xylem. Inhibition at this site was also found to be greatest in low phosphorus status plants. From these results it is suggested that plants of low phosphorus status possess high affinity phosphate xylem loading mechanisms, induced under conditions of phosphorus deficiency, which have a greater susceptibility to arsenate competitive inhibition and toxicity than equivalent xylem loading mechanisms in high phosphorus status plants

Spechts Ferry (Middle Ordovician) bryozoan fauna from Illinois, Wisconsin, and Iowa.

Information circular / Illinois State Geological

On the Struggle To Attain Universal Competence in a Complex Skill: The Case of a Senior Capstone Experience

Seventeen years ago, a blue-ribbon committee of economists asserted that the “ideal” program for an economics major should include “a capstone experience, whereby students apply their knowledge and skills in creative and systematic ways through research and writing.” (Seigfried, Bartlett, Hansen, Kelley, McCloskey, and Tietenberg (1991)) Yet we are not aware of a data-driven assessment of an implemented senior capstone experience in the economics education literature. We have been experimenting with a senior capstone experience – based on research and writing – for more than a decade. In this paper we describe the history of our senior capstone experience, and provide a data-driven assessment of student performance in this experience. Our primary objective for this senior capstone has been to create a system that will result in all of our majors writing a competent senior thesis. In other words, we want to attain universal competence in a complex skill. This simple objective has, however, been very difficult to achieve. We will argue that undesired variation in student motivation has been our primary obstacle in attaining universal competence.

The U.S. and Irish Credit Crises: Their Distinctive Differences and Common Features

Abstract: Although the US credit crisis precipitated it, the Irish credit crisis is an identifiably separate one, which might have occurred in the absence of the U.S. crash. The distinctive differences between them are notable. Almost all the apparent causal factors of the U.S. crisis are missing in the Irish case; and the same applies vice-versa. At a deeper level, we identify four common features of the two credit crises: capital bonanzas, irrational exuberance, regulatory imprudence, and moral hazard. The particular manifestations of these four “deep” common features are quite different in the two cases.

Characterizing droplet combustion of pure and multi-component liquid fuels in a microgravity environment

The importance of understanding the effects of fuel composition, length scales, and other parameters on the combustion of liquid fuels has motivated the examination of simple flames which have easily characterized flow fields and hence, the potential of being modeled accurately. One such flame for liquid fuel combustion is the spherically symmetric droplet flame which can be achieved in an environment with sufficiently low gravity (i.e., low buoyancy). To examine fundamental characteristics of spherically symmetric droplet combustion, a drop tower facility has been employed to provide a microgravity environment to study droplet combustion. This paper gives a brief review of results obtained over the past three years under NASA sponsorship (grant NAG3-987)

Distribution of Values of Quadratic Forms at Integral Points

The number of lattice points in $d$-dimensional hyperbolic or elliptic shells $\{m : a<Q[m]<b\}$, which are restricted to rescaled and growing domains $r\;\Omega$, is approximated by the volume. An effective error bound of order $o(r^{d-2})$ for this approximation is proved based on Diophantine approximation properties of the quadratic form $Q$. These results allow to show effective variants of previous non-effective results in the quantitative Oppenheim problem and extend known effective results in dimension $d \geq 9$ to dimension $d \geq 5$. They apply to wide shells when $b-a$ is growing with $r$ and to positive definite forms $Q$. For indefinite forms they provide explicit bounds (depending on the signature or Diophantine properties of $Q$) for the size of non-zero integral points $m$ in dimension $d\geq 5$ solving the Diophantine inequality $|Q[m]| < \varepsilon$ and provide error bounds comparable with those for positive forms up to powers of $\log r$.Comment: Section 4 and 7, also bounds in sections 6 have been revised and greatly extended, e.g. box artifacts for wide hyperbolic shells are removed. Applications to Diophantine lattices are revised and reduced exponents for solutions of Diophantine inequalities depending on signature are proved for large $d$, similar to integral forms. For small $d$ larger exponents are now required in Th.1.