Transcriptional profile of Salmonella enterica subsp. enterica serovar Weltevreden during alfalfa sprout colonization

Sprouted seeds represent a great risk for infection by human enteric pathogens because of favourable growth conditions for pathogens during their germination. The aim of this study was to identify mechanisms of interactions of Salmonella enterica subsp. enterica Weltevreden with alfalfa sprouts. RNA-seq analysis of S. Weltevreden grown with sprouts in comparison with M9-glucose medium showed that among a total of 4158 annotated coding sequences, 177 genes (4.3%) and 345 genes (8.3%) were transcribed at higher levels with sprouts and in minimal medium respectively. Genes that were higher transcribed with sprouts are coding for proteins involved in mechanisms known to be important for attachment, motility and biofilm formation. Besides gene expression required for phenotypic adaption, genes involved in sulphate acquisition were higher transcribed, suggesting that the surface on alfalfa sprouts may be poor in sulphate. Genes encoding structural and effector proteins of Salmonella pathogenicity island 2, involved in survival within macrophages during infection of animal tissue, were higher transcribed with sprouts possibly as a response to environmental conditions. This study provides insight on additional mechanisms that may be important for pathogen interactions with sprouts

The construct validity of the Dutch personality inventory for DSM-5 personality disorders (PID-5) in a clinical sample

The factor structure and the convergent validity of the Personality Inventory for DSM-5 (PID-5), a self-report questionnaire designed to measure personality pathology as advocated in the fifth edition, Section III of Diagnostic and Statistical Manual of Mental Disorders (DSM-5), are already demonstrated in general population samples, but need replication in clinical samples. In 240 Flemish inpatients, we examined the factor structure of the PID-5 by means of exploratory structural equation modeling. Additionally, we investigated differences in PID-5 higher order domain scores according to gender, age and educational level, and explored convergent and discriminant validity by relating the PID-5 with the Dimensional Assessment of Personality PathologyBasic Questionnaire and by comparing PID-5 scores of inpatients with and without a DSM-IV categorical personality disorder diagnosis. Our results confirmed the original five-factor structure of the PID-5. The reliability and the convergent and discriminant validity of the PID-5 proved to be adequate. Implications for future research are discussed

Bilaterale osteochondritis dissecans van de laterale taluskam bij een jonge rottweiler

In this case report, a young Rottweiler is described that suffered from bilateral osteochondritis dissecans of the talus. The signalment, history and clinical signs were typical for this condition, but atypically, the lateral trochlear ridges were affected. Due to the large size of the fragments, the prognosis was reserved. However, one year after surgery, the clinical outcome was surprisingly good. The left and right trochlear ridges seemed to be filled up with osseous material

First geodetic observations using new VLBI stations ASKAP-29 and WARK12M

We report the results of a successful 7 hour 1.4 GHz VLBI experiment using two new stations, ASKAP-29 located in Western Australia and WARK12M located on the North Island of New Zealand. This was the first geodetic VLBI observing session with the participation of these new stations. We have determined the positions of ASKAP-29 and WARK12M. Random errors on position estimates are 150-200 mm for the vertical component and 40-50 mm for the horizontal component. Systematic errors caused by the unmodeled ionosphere path delay may reach 1.3 m for the vertical component.Comment: 11 pages, 6 flgures, 4 table

The effect of graphite and carbon black ratios on conductive ink performance

Conductive inks based on graphite and carbon black are used in a host of applications including energy storage, energy harvesting, electrochemical sensors and printed heaters. This requires accurate control of electrical properties tailored to the application; ink formulation is a fundamental element of this. Data on how formulation relates to properties have tended to apply to only single types of conductor at any time, with data on mixed types of carbon only empirical thus far. Therefore, screen printable carbon inks with differing graphite, carbon black and vinyl polymer content were formulated and printed to establish the effect on rheology, deposition and conductivity. The study found that at a higher total carbon loading ink of 29.4% by mass, optimal conductivity (0.029 Ω cm) was achieved at a graphite to carbon black ratio of 2.6 to 1. For a lower total carbon loading (21.7 mass %), this ratio was reduced to 1.8 to 1. Formulation affected viscosity and hence ink transfer and also surface roughness due to retention of features from the screen printing mesh and the inherent roughness of the carbon components, as well as the ability of features to be reproduced consistently

New insights into North European and North Atlantic surface pressure variability, storminess, and related climatic change since 1830

The authors present initial results of a new pan-European and international storminess since 1800 as interpreted from European and North Atlantic barometric pressure variability (SENABAR) project. This first stage analyzes results of a new daily pressure variability index, dp(abs)24, from long-running meteorological stations in Denmark, the Faroe Islands, Greenland, Iceland, the United Kingdom, and Ireland, some with data from as far back as the 1830s. It is shown that dp(abs)24 is significantly related to wind speed and is therefore a good measure of Atlantic and Northwest European storminess and climatic variations. The authors investigate the temporal and spatial consistency of dp(abs)24, the connection between annual and seasonal dp(abs)24 and the North Atlantic Oscillation Index (NAOI), as well as dp(abs)24 links with historical storm records. The results show periods of relatively high dp(abs)24 and enhanced storminess around 1900 and the early to mid-1990s, and a relatively quiescent period from about 1930 to the early 1960s, in keeping with earlier studies. There is little evidence that the mid- to late nineteenth century was less stormy than the present, and there is no sign of a sustained enhanced storminess signal associated with "global warming." The results mark the first step of a project intending to improve on earlier work by linking barometric pressure data from a wide network of stations with new gridded pressure and reanalysis datasets, GCMs, and the NAOI. This work aims to provide much improved spatial and temporal coverage of changes in European, Atlantic, and global storminess. © 2008 American Meteorological Society

The design and construction of a PhET-based inquiry learning worksheet to develop understanding of the particle-in-a-box model

We report on the design and construction of a worksheet to develop upper secondary school students' understanding of the particle-in-a-box model. We designed a worksheet that guided students' structured-inquiry learning through peer discussion using the PhET simulation 'Quantum Bound States'. The worksheet was improved in three iterative cycles of (re)designing, testing and evaluating, leading to a validated design and tentative design principles. Students' discourse was recorded whilst they were using the worksheet and the PhET simulation in the test phase of each cycle. Analyses of students' discourse informed the redesign of the worksheet for each subsequent cycle, until the design was finalised. The results showed the potential of the simulation to introduce upper secondary school students to the particle-in-a-box model, provided care is taken to accompany student inquiry with a well-developed worksheet as learning support during the lesson

Review: The role of prolactin in the maternal investment-survival balance

Prolactin is a protein hormone that plays a regulating role in many physiological processes across mammalian species, but the two primary functions it exerts are the development of the mammary glands and milk production. This review will focus on the potential key role of prolactin in the hormonal control of maternal investment and survival in mammals. Prolactin has been demonstrated to influence maternal investment throughout the complete investment process from ovulation and implantation to gestation and lactation, highlighting its essential role in this balance. Different stressors may affect prolactin secretion, and therefore, be a trigger to lower or stop maternal investment. Hence, comprehending prolactin's function in maternal investment and survival strategies can have important implications for our understanding of reproductive success and failure, potentially benefiting conservation efforts to protect endangered species. Since the role of prolactin is inconsistent in different mammalian species, species-specific research is mandatory to determine the exact role of prolactin in individual maternal survival-investment strategies. The current knowledge on this topic is highlighted in the present review but is also illustrative of the fact that many areas of prolactin related research remain underexposed

Complete genome sequence of the fire blight pathogen Erwinia pyrifoliae DSM 12163T and comparative genomic insights into plant pathogenicity

Smits THM, Jaenicke S, Rezzonico F, et al. Complete genome sequence of the fire blight pathogen Erwinia pyrifoliae DSM 12163(T) and comparative genomic insights into plant pathogenicity. BMC Genomics. 2010;11(1): 2.Background: Erwinia pyrifoliae is a newly described necrotrophic pathogen, which causes fire blight on Asian (Nashi) pear and is geographically restricted to Eastern Asia. Relatively little is known about its genetics compared to the closely related main fire blight pathogen E. amylovora. Results: The genome of the type strain of E. pyrifoliae strain DSM 12163(T), was sequenced using both 454 and Solexa pyrosequencing and annotated. The genome contains a circular chromosome of 4.026 Mb and four small plasmids. Based on their respective role in virulence in E. amylovora or related organisms, we identified several putative virulence factors, including type III and type VI secretion systems and their effectors, flagellar genes, sorbitol metabolism, iron uptake determinants, and quorum-sensing components. A deletion in the rpoS gene covering the most conserved region of the protein was identified which may contribute to the difference in virulence/host-range compared to E. amylovora. Comparative genomics with the pome fruit epiphyte Erwinia tasmaniensis Et1/99 showed that both species are overall highly similar, although specific differences were identified, for example the presence of some phage gene-containing regions and a high number of putative genomic islands containing transposases in the E. pyrifoliae DSM 12163T genome. Conclusions: The E. pyrifoliae genome is an important addition to the published genome of E. tasmaniensis and the unfinished genome of E. amylovora providing a foundation for re-sequencing additional strains that may shed light on the evolution of the host-range and virulence/pathogenicity of this important group of plant-associated bacteria

New insights into North European and North Atlantic surface pressure variability, storminess and related climate change since 1830

The authors present initial results of a new pan-European and international storminess since 1800 as interpreted from European and North Atlantic barometric pressure variability (SENABAR) project. This first stage analyzes results of a new daily pressure variability index, dp(abs)24, from long-running meteorological stations in Denmark, the Faroe Islands, Greenland, Iceland, the United Kingdom, and Ireland, some with data from as far back as the 1830s. It is shown that dp(abs)24 is significantly related to wind speed and is therefore a good measure of Atlantic and Northwest European storminess and climatic variations. The authors investigate the temporal and spatial consistency of dp(abs)24, the connection between annual and seasonal dp(abs)24 and the North Atlantic Oscillation Index (NAOI), as well as dp(abs)24 links with historical storm records. The results show periods of relatively high dp(abs)24 and enhanced storminess around 1900 and the early to mid-1990s, and a relatively quiescent period from about 1930 to the early 1960s, in keeping with earlier studies. There is little evidence that the mid- to late nineteenth century was less stormy than the present, and there is no sign of a sustained enhanced storminess signal associated with “global warming.” The results mark the first step of a project intending to improve on earlier work by linking barometric pressure data from a wide network of stations with new gridded pressure and reanalysis datasets, GCMs, and the NAOI. This work aims to provide much improved spatial and temporal coverage of changes in European, Atlantic, and global storminess