Surface reactivity of amphibole asbestos. A comparison between crocidolite and tremolite

Among asbestos minerals, fibrous riebeckite (crocidolite) and tremolite share the amphibole structure but largely differ in terms of their iron content and oxidation state. In asbestos toxicology, iron-generated free radicals are largely held as one of the causes of asbestos malignant effect. With the aim of clarifying i) the relationship between Fe occurrence and asbestos surface reactivity, and ii) how free-radical generation is modulated by surface modifications of the minerals, UICC crocidolite and fibrous tremolite from Maryland were leached from 1 day to 1 month in an oxidative medium buffered at pH 7.4 to induce redox alterations and surface rearrangements that may occur in body fluids. Structural and chemical modifications and free radical generation were monitored by HR-TEM/EDS and spin trapping/EPR spectroscopy, respectively. Free radical yield resulted to be dependent on few specific Fe2+ and Fe3+ surface sites rather than total Fe content. The evolution of reactivity with time highlighted that low-coordinated Fe ions primarily contribute to the overall reactivity of the fibre. Current findings contribute to explain the causes of the severe asbestosinduced oxidative stress at molecular level also for iron-poor amphiboles, and demonstrate that asbestos have a sustained surface radical activity even when highly altered by oxidative leaching

Simultaneous localization and odometry self calibration for mobile robot

This paper presents both the theory and the experimental results of a method allowing simultaneous robot localization and odometry error estimation (both systematic and non-systematic) during the navigation. The estimation of the systematic components is carried out through an augmented Kalman filter, which estimates a state containing the robot configuration and the parameters characterizing the systematic component of the odometry error. It uses encoder readings as inputs and the readings from a laser range finder as observations. In this first filter, the non-systematic error is defined as constant and it is overestimated. Then, the estimation of the real non-systematic component is carried out through another Kalman filter, where the observations are obtained by two subsequent robot configurations provided by the previous augmented Kalman filter. There, the systematic parameters in the model are regularly updated with the values estimated by the first filter. The approach is theoretically developed for both the synchronous and the differential drive. A first validation is performed through very accurate simulations where both the drive systems are considered. Then, a series of experiments are carried out in an indoor environment by using a mobile platform with a differential driv

La ciencia según futuros profesores : entre la tradición y la novedad

Este trabajo se enmarca en una investigación que tiene por objetivo diseñar y estudiar escenarios de formación de docentes de ciencia, situados en contextos institucionales diversos. La perspectiva teórica asumida recoge los enfoques actuales acerca de la formación docente y el pensamiento del profesor; complementados con una visión que reconoce el valor del discurso en la construcción, no sólo del pensamiento, sino también de las realidades en que se insertan los sujetos. En este trabajo, tomamos uno de los escenarios de FD implementado con un grupo de estudiantes de profesorado y analizamos secuencias didácticas (SD) que éstos elaboran para la enseñanza de un contenido escolar de ciencias. Lo que nos interesa conocer es cómo, a través del discurso, los estudiantes construyen modos particulares de concebir la ciencia y cómo proyectan prácticas docentes al respecto

La ciencia según futuros profesores : entre la tradición y la novedad

Este trabajo se enmarca en una investigación que tiene por objetivo diseñar y estudiar escenarios de formación de docentes de ciencia, situados en contextos institucionales diversos. La perspectiva teórica asumida recoge los enfoques actuales acerca de la formación docente y el pensamiento del profesor; complementados con una visión que reconoce el valor del discurso en la construcción, no sólo del pensamiento, sino también de las realidades en que se insertan los sujetos. En este trabajo, tomamos uno de los escenarios de FD implementado con un grupo de estudiantes de profesorado y analizamos secuencias didácticas (SD) que éstos elaboran para la enseñanza de un contenido escolar de ciencias. Lo que nos interesa conocer es cómo, a través del discurso, los estudiantes construyen modos particulares de concebir la ciencia y cómo proyectan prácticas docentes al respecto

Evidence for repetitive load in the trapezius muscle during a tapping task

Many studies describe the trapezius muscle activation pattern during repetitive key-tapping focusing on continuous activation. The objectives of this study were to determine whether the upper trapezius is phasically active during supported key tapping, whether this activity is cross-correlated with forearm muscle activity, and whether trapezius activity depends on key characteristic. Thirteen subjects (29.7±11.4years) were tested. Surface EMG of the finger's extensor and flexor and of the trapezius muscles, as well as the key on-off signal was recorded while the subject performed a 2-min session of key tapping at 4Hz. The linear envelopes obtained were cut into single tapping cycles extending from one onset to the next onset signal and subsequently time-normalized. Effect size between mean range and maximal standard deviation was calculated to determine as to whether a burst of trapezius muscle activation was present. Cross-correlation was used to determine the time-lag of the activity bursts between forearm and trapezius muscles. For each person the mean and standard deviation of the cross-correlations coefficient between forearm muscles and trapezius were determined. Results showed a burst of activation in the trapezius muscle during most of the tapping cycles. The calculated effect size was ≥0.5 in 67% of the cases. Cross-correlation factors between forearm and trapezius muscle activity were between 0.75 and 0.98 for both extensor and flexor muscles. The cross-correlated phasic trapezius activity did not depend on key characteristics. Trapezius muscle was dynamically active during key tapping; its activity was clearly correlated with forearm muscles' activit

A comparison of line extraction algorithms using 2D range data for indoor mobile robotics

This paper presents an experimental evaluation of different line extraction algorithms applied to 2D laser scans for indoor environments. Six popular algorithms in mobile robotics and computer vision are selected and tested. Real scan data collected from two office environments by using different platforms are used in the experiments in order to evaluate the algorithms. Several comparison criteria are proposed and discussed to highlight the advantages and drawbacks of each algorithm, including speed, complexity, correctness and precision. The results of the algorithms are compared with ground truth using standard statistical methods. An extended case study is performed to further evaluate the algorithms in a SLAM applicatio

Secure Communication in Vehicular Networks - PRESERVE Demo

Security and privacy are fundamental prerequisites for the deployment of vehicular communications. The near-deployment status of Safety Applications for Intelligent Transport Systems (ITS) calls for strong evidence on the applicability of proposed research solutions, notably close-to-reality situations and field-operational trials. The contribution of our work is in this direction: We present a demonstration of the integration and the interoperability among components and security mechanisms coming from different Research and Development projects, as per the PRESERVE project. In fact, we show that the components of the SeVeCom and EVITA projects with the PRESERVE architecture lead to strong and practical security and privacy solutions for Vehicular Ad-hoc Networks (VANETs)

Secretagogue stimulation of neurosecretory cells elicits filopodial extensions uncovering new functional release sites

Regulated exocytosis in neurosecretory cells relies on the timely fusion of secretory granules (SGs) with the plasma membrane. Secretagogue stimulation leads to an enlargement of the cell footprint (surface area in contact with the coverslip), an effect previously attributed to exocytic fusion of SGs with the plasma membrane. Using total internal reflection fluorescence microscopy, we reveal the formation of filopodia-like structures in bovine chromaffin and PC12 cells driving the footprint expansion, suggesting the involvement of cortical actin network remodeling in this process. Using exocytosis-incompetent PC12 cells, we demonstrate that footprint enlargement is largely independent of SG fusion, suggesting that vesicular exocytic fusion plays a relatively minor role in filopodial expansion. The footprint periphery, including filopodia, undergoes extensive F-actin remodeling, an effect abolished by the actomyosin inhibitors cytochalasin D and blebbistatin. Imaging of both Lifeact-GFP and the SG marker protein neuropeptide Y-mCherry reveals that SGs actively translocate along newly forming actin tracks before undergoing fusion. Together, these data demonstrate that neurosecretory cells regulate the number of SGs undergoing exocytosis during sustained stimulation by controlling vesicular mobilization and translocation to the plasma membrane through actin remodeling. Such remodeling facilitates the de novo formation of fusion sites

Toxicity of boehmite nanoparticles: impact of the ultrafine fraction and of the agglomerates size on cytotoxicity and pro-inflammatory response

International audienceBoehmite (γ-AlOOH) nanoparticles (NPs) are used in a wide range of industrial applications. However, little is known about their potential toxicity. This study aimed at a better understanding of the relationship between the physico-chemical properties of these NPs and their in vitro biological activity. After an extensive physico-chemical characterization, the cytotoxicity, pro-inflammatory response and oxidative stress induced by a bulk industrial powder and its ultrafine fraction were assessed using RAW264.7 macrophages. Although the bulk powder did not trigger a significant biological activity, pro-inflammatory response was highly enhanced with the ultrafine fraction. This observation was confirmed with boehmite NPs synthesized at the laboratory scale, with well-defined and tightly controlled physico-chemical features: toxicity was increased when NPs were dispersed. In conclusion, the agglomerates size of boehmite NPs has a major impact on their toxicity, highlighting the need to study not only raw industrial powders containing NPs but also the ultrafine fractions representative of respirable particles. Read More: http://informahealthcare.com/doi/abs/10.3109/08958378.2014.92599