Transcription factor scleraxis vitally contributes to progenitor lineage direction in wound healing of adult tendon in mice

Tendon is a dense connective tissue that transmits high mechanical forces from skeletal muscle to bone. The transcription factor scleraxis (Scx) is a highly specific marker of both precursor and mature tendon cells (tenocytes). Mice lacking scx exhibit a specific and virtually complete loss of tendons during development. However, the functional contribution of Scx to wound healing in adult tendon has not yet been fully characterized. Here, using ScxGFP-tracking and loss-of-function systems, we show in an adult mouse model of Achilles tendon injury that paratenon cells, representing a stem cell antigen-1 (Sca-1)–positive and Scx-negative progenitor subpopulation, display Scx induction, migrate to the wound site, and produce extracellular matrix (ECM) to bridge the defect, whereas resident tenocytes exhibit a delayed response. Scx induction in the progenitors is initiated by transforming growth factor β (TGF-β) signaling. scx-deficient mice had migration of Sca-1–positive progenitor cell to the lesion site but impaired ECM assembly to bridge the defect. Mechanistically, scx-null progenitors displayed higher chondrogenic potential with up-regulation of SRY-box 9 (Sox9) coactivator PPAR-γ coactivator-1α (PGC-1α) in vitro, and knock-in analysis revealed that forced expression of full-length scx significantly inhibited Sox9 expression. Accordingly, scx-null wounds formed cartilage-like tissues that developed ectopic ossification. Our findings indicate a critical role of Scx in a progenitor-cell lineage in wound healing of adult mouse tendon. These progenitor cells could represent targets in strategies to facilitate tendon repair. We propose that this lineage-regulatory mechanism in tissue progenitors could apply to a broader set of tissues or biological systems in the body

Special Relativistic Simulations of Magnetically-dominated Jets in Collapsing Massive Stars

We perform a series of two-dimensional magnetohydrodynamic core-collapse simulations of rapidly rotating and strongly magnetized massive stars. To study the properties of magnetic explosions for a longer time stretch of postbounce evolution, we develop a new code under the framework of special relativity including a realistic equation of state with a multiflavor neutrino leakage scheme. Our results show the generation of the magnetically-dominated jets in the two ways. One is launched just after the core-bounce in a prompt way and another is launched at $\sim 100$ ms after the stall of the prompt shock. We find that the shock-revival occurs when the magnetic pressure becomes strong, due to the field wrapping, enough to overwhelm the ram pressure of the accreting matter. The critical toroidal magnetic fields for the magnetic shock-revival are found to be universal of $\sim 10^{15}\mathrm{G}$ behind the jets. We point out that the time difference before the shock-revival has a strong correlation with the explosions energies. Our results suggest that the magnetically dominated jets are accompanied by the formation of the magnetars. Since the jets are mildly relativistic, we speculate that they might be the origin of some observed X-ray flashes.Comment: 50 pages, 14 figures, Accepted to ApJ, A paper with high-resolution figures available at http://www-utap.phys.s.u-tokyo.ac.jp/~takiwaki/res/index-j.htm

Post-event Processing Predicts Cortisol Recovery

There is growing evidence that individuals with social anxiety show impaired cortisol recovery after experiencing social evaluative stressors. Yet, little is known regarding the cognitive processes underlying such impaired cortisol recovery. The present study examined the effect of post-event processing (PEP), referred to as repetitive thinking about social situations, on cortisol recovery following a social stressor. Forty-two non-clinical university students (23 women, 19 men, mean age = 22.0 ± 2.0 years) completed the Trier Social Stress Test (TSST), followed by a thought sampling procedure which assessed the frequency of PEP reflecting the TSST. A growth curve model showed PEP and social anxiety interactively predicted cortisol recovery. In particular, PEP predicted impaired cortisol recovery in those with low levels of social anxiety but not in those with high levels of social anxiety, which contradicted the initial hypothesis. These findings suggest that PEP is differentially associated with cortisol recovery depending on levels of social anxiety. The possible mechanisms underlying these findings were discussed in terms of protective inhibition framework

VLBI Astrometry of AGB Variables with VERA -- A Semiregular Variable S Crateris --

We present a distance measurement for the semiregular variable S Crateris (S Crt) based on its annual parallax. With the unique dual beam system of the VLBI Exploration for Radio Astrometry (VERA) telescopes, we measured the absolute proper motion of a water maser spot associated with S Crt, referred to the quasar J1147-0724 located at an angular separation of 1.23$^{\circ}$. In observations spanning nearly two years, we have detected the maser spot at the LSR velocity of 34.7 km s$^{-1}$, for which we measured the annual parallax of 2.33$\pm$0.13 mas corresponding to a distance of 430$^{+25}_{-23}$ pc. This measurement has an accuracy one order of magnitude better than the parallax measurements of HIPPARCOS. The angular distribution and three-dimensional velocity field of maser spots indicate a bipolar outflow with the flow axis along northeast-southwest direction. Using the distance and photospheric temperature, we estimate the stellar radius of S Crt and compare it with those of Mira variables.Comment: 9 pages, 4 figures, accepted for publication in PASJ (Vol.60, No.5, October 25, VERA special issue

Long-term Evolution of a Supernova Remnant Hosting a Double Neutron Star Binary

An ultra-stripped supernova (USSN) is a type of core-collapse supernova explosion proposed to be a candidate formation site of a double neutron star (DNS) binary. We investigate the dynamical evolution of an ultra-stripped supernova remnant (USSNR), which should host a DNS at its center. By accounting for the mass-loss history of the progenitor binary using a model developed by a previous study, we construct the large-scale structure of the circumstellar medium (CSM) up to a radius ∼100 pc, and simulate the explosion and subsequent evolution of a USSN surrounded by such a CSM environment. We find that the CSM encompasses an extended region characterized by a hot plasma with a temperature ∼10⁸ K located around the termination shock of the wind from the progenitor binary (∼10 pc), and the USSNR blast wave is drastically weakened while penetrating through this hot plasma. Radio continuum emission from a young USSNR is sufficiently bright to be detectable if it inhabits our galaxy but faint compared to the observed Galactic supernova remnants (SNRs), and thereafter declines in luminosity through adiabatic cooling. Within our parameter space, USSNRs typically exhibit a low radio luminosity and surface brightness compared to the known Galactic SNRs. Due to the small event rate of USSNe and their relatively short observable life span, we calculate that USSNRs account for only ∼0.1%–1% of the total SNR population. This is consistent with the fact that no SNR hosting a DNS binary has been discovered in the Milky Way so far

TAT-dextran-mediated mitochondrial transfer enhances recovery from models of reperfusion injury in cultured cardiomyocytes

Acute myocardial infarction is a leading cause of death among single organ diseases. Despite successful reperfusion therapy, ischaemia reperfusion injury (IRI) can induce oxidative stress (OS), cardiomyocyte apoptosis, autophagy and release of inflammatory cytokines, resulting in increased infarct size. In IRI, mitochondrial dysfunction is a key factor, which involves the production of reactive oxygen species, activation of inflammatory signalling cascades or innate immune responses, and apoptosis. Therefore, intercellular mitochondrial transfer could be considered as a promising treatment strategy for ischaemic heart disease. However, low transfer efficiency is a challenge in clinical settings. We previously reported uptake of isolated exogenous mitochondria into cultured cells through co-incubation, mediated by macropinocytosis. Here, we report the use of transactivator of transcription dextran complexes (TAT-dextran) to enhance cellular uptake of exogenous mitochondria and improve the protective effect of mitochondrial replenishment in neonatal rat cardiomyocytes (NRCMs) against OS. TAT-dextran-modified mitochondria (TAT-Mito) showed a significantly higher level of cellular uptake. Mitochondrial transfer into NRCMs resulted in anti-apoptotic capability and prevented the suppression of oxidative phosphorylation in mitochondria after OS. Furthermore, TAT-Mito significantly reduced the apoptotic rates of cardiomyocytes after OS, compared to simple mitochondrial transfer. These results indicate the potential of mitochondrial replenishment therapy in OS-induced myocardial IRI