Istraživanje medija različitog sadržaja na organiziranje meristema in vitro

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l-1 myo-inositol, 2 mg·l-1 thiamine-HCl, 1 mg·l-1 piridoxine-HCl, 1 mg·l-1 nicotinic acid and 30 g·l-1 sucrose and 8 g·l-1 agar was considered together with two concentrations of BA (0,2 and 2 mg·l-1). Half strength MS medium was also used. There was a significant variation of the proliferated shoots, depending on media. We got high level of rooting and we have investigated acclimatization of regenerants grown on sterile substrate in conditions of high humidity.Istraživani su optimalni uvjeti za razmanžanje i ukorjenjivanje kultivara Willamette in vitro. Visok stupanj proliferacije i razmanžanja nakon 90 dana kulture na Murashige i Skoog (MS) mediju sa 100 mg·l-1 mio inositola, 2 mg·l-1 tijamina-HCl, 1 mg·l-1 piridoksina-HCl, 1 mg·l-1 nikotinske kiseline, 30 g·l-1 saharoze i 8 g·l-1 agara razmatran je s dvije koncentracije BA (0,2 i 2,0 mg·l-1). Primijenjen je i medij MS pola koncentracije. Značajno je bilo odstupanje proliferiranih izboja, ovisno o mediju. Dobili smo visoku razinu ukorjenjenja te smo istražili prilagodbu ponovno naraslih izboja na sterilnom supstratu u uvjetima obilne vlage

Istraživanje medija različitog sadržaja na organiziranje meristema in vitro

Optimal conditions for in vitro multiplication and rooting of cv. Willamette were investigated. High level of proliferation and multiplication after 90 days of culture on Murashige and Skoog (MS) medium with 100 mg·l-1 myo-inositol, 2 mg·l-1 thiamine-HCl, 1 mg·l-1 piridoxine-HCl, 1 mg·l-1 nicotinic acid and 30 g·l-1 sucrose and 8 g·l-1 agar was considered together with two concentrations of BA (0,2 and 2 mg·l-1). Half strength MS medium was also used. There was a significant variation of the proliferated shoots, depending on media. We got high level of rooting and we have investigated acclimatization of regenerants grown on sterile substrate in conditions of high humidity.Istraživani su optimalni uvjeti za razmanžanje i ukorjenjivanje kultivara Willamette in vitro. Visok stupanj proliferacije i razmanžanja nakon 90 dana kulture na Murashige i Skoog (MS) mediju sa 100 mg·l-1 mio inositola, 2 mg·l-1 tijamina-HCl, 1 mg·l-1 piridoksina-HCl, 1 mg·l-1 nikotinske kiseline, 30 g·l-1 saharoze i 8 g·l-1 agara razmatran je s dvije koncentracije BA (0,2 i 2,0 mg·l-1). Primijenjen je i medij MS pola koncentracije. Značajno je bilo odstupanje proliferiranih izboja, ovisno o mediju. Dobili smo visoku razinu ukorjenjenja te smo istražili prilagodbu ponovno naraslih izboja na sterilnom supstratu u uvjetima obilne vlage