Radiosensitization by telomerase-dependent oncolytic adenovirus

DNA修復機能阻害は放射線感受性を増強させるため，DNA修復に関与する因子の阻害剤は放射線増感剤となり得る．我々の開発したテロメラーゼ依存的腫瘍融解アデノウイルス製剤OBP-301（テロメライシン）は，アデノウイルスE1B55kDaタンパクを介して細胞のDNA修復に重要な役割を果たすMRN複合体（Mre11，Rad50，NBS1）を分解する機能を有する．このMRN複合体の分解によりATM（ataxia-telangiectasia mutated）の活性化が抑制され結果的にDNA修復機構が阻害される．我々はOBP-301と放射線との併用が強力な相乗効果を生み出すことをマウスの皮下腫瘍モデルおよび食道癌同所性モデルにおいて証明した．これらの結果はOBP-301が将来有望な放射線増感剤となり得ることだけでなく，E1B55kDaタンパクを産生する腫瘍融解アデノウイルス製剤と放射線との併用が悪性腫瘍に対する有力な治療戦略となり得ることを示す

Carbon, nitrogen and phosphorus contents of cultured Nori (Porphyra yezoensis)

有明海佐賀県沿岸で採取された紅藻スサビノリを用いて,炭素,窒素,リン含有量を測定した。炭素,窒素,リンの含有量は,それぞれ42.6±2.3μg-at mg DW^4, 8.6±0.8μg-at mg DW^,0.32±0.01μg-at mg DW^ であり,それらの比はC:N:P=132:26:1であった。Carbon, nitrogen and phosphorus contents of cultured Nori (Porphyra yezoensis) that were sampled from Saga sea region of Ariake Bay, were measured. Carbon, nitrogen and phosphorus contents of the frond were 42.6±2.3, 8.6±0.8, 0.32±0.01μg-at mg DW^ respectively, and the ratio was C:N:P=132:26:1原著論

The Role of Seaweed Bed for the Lower Trophic Ecosystem in Coastal Seas

藻場は高い基礎生産力を持っており，沿岸海域の低次生態系に対して重要な役割を果たしていると考えられる。本研究では，瀬戸内海の各海域における藻場と水中植物プランクトンとの生産力の比較を行った。水中植物プランクトン生産量に対する藻場生産量の割合は安芸灘で最も高く（10.8％），ついで備讃瀬戸で高い値を示した（3.8％）。一方，広島湾，大阪湾，播磨灘では低い値であった（0.6, 0.3, 0.2％）。季節毎に算出した水中植物プランクトン生産量に対する藻場生産量の割合は冬季に高い割合であった。また，広島湾南部海域を対象海域として，藻場の栄養塩の吸収量・放出量・蓄積量を算出し，栄養塩収支における藻場の役割を定量的に明らかにした。その結果，窒素，リンそれぞれ河川負荷量の14, 40％に相当する量が難分解性有機物として蓄積されていることが明らかになった。Seaweed bed has an important role for the lower trophic ecosystem in coastal seas because of its high primary production. In this study, the primary production of the seaweed bed and the pelagic phytoplankton was compared in each sub area of the Seto Inland Sea. The ratio of the primary production of seaweed bed to pelagic phytoplankton was high at Aki-Nada (10.8 %) and Bisan-Seto (3.8 %), while the ratio was low at Hiroshima-Bay (0.6 %), Osaka-Bay (0.3 %) and Harima-Nada (0.2 %). Seasonally, the ratio was high during winter. The uptake rate by primary production, release rate by decomposition and stock rate as a leaves of nutrients by the seaweed bed were estimated in the southern area of Hiroshima-Bay. The quantitative contribution of seagrass bed to the nutrient budget in this area was estimated. It was estimated that 14 and 40 % of load from the river were accumulated as the less degradable organic matter for nitrogen and phosphorus, respectively

Lack of Modifying Effects of Intratracheal Instillation of Quartz or Dextran Sulfate Sodium (DSS) in Drinking Water on Lung Tumor Development Initiated with 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in Female A/J Mice

The purpose of the present study was to investigate the effects of inflammation, induced by intratracheal instillation (i.t.) of quartz as an environmental factor in the lung or drinking of dextran sulfate sodium (DSS) as an environmental factor in the colon on lung tumors in female A/J mice initiated with NNK. For comparison, colonic preneoplastic lesions, aberrant crypt foci (ACF), were also assessed. A/J mice at 6 weeks of age were divided into 5 groups, and Groups 1, 2 and 3 were pretreated with NNK (2 mg / 0.1 ml saline / mouse, intraperitoneal injection) at week 0. For a week, 2% DSS in drinking water was administered to the mice in Groups 2 and 4 beginning in week 1. In week 2, the mice of Groups 3 and 5 were exposed to intratracheal instillation of quartz (0.1 mg/rat) suspended in 25 μl saline. The experiment was terminated after 16 weeks. The results for the lung tumors and colonic ACFs showed a lack of modifying effects of the inflammation in either site. Hematologically and histopathologically, the inflammation induced by 0.1 mg quartz in the lung and 2% DSS in the colon was lacking or only mild at the end of 16 weeks. These results suggest that there may be differences in sensitivity to inflammation that determine tumor promoting potential