3,694 research outputs found

    In Vivo Localization of Fas-Associated Death Domain Protein in the Nucleus and Cytoplasm of Normal Thyroid and Liver Cells

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    FADD (Fas-associated death domain) is the main death receptor adaptor molecule that transmits apoptotic signal. Recently, FADD protein was shown to be expressed both in the cytoplasm and nucleus of in vitro cell lines. In contrast to the cytoplasmic FADD, the nuclear FADD was shown to protect cells from apoptosis. However, in vivo subcellular localization of FADD was still unknown. Here, we demonstrated that FADD protein was expressed in both cytoplasmic and nuclear compartment in ex vivo thyroid cells demonstrating that nuclear sublocalization of FADD protein was a relevant phenomenon occurring in vivo. Moreover, we showed that in the nucleus of untransformed thyroid cells FADD localized mainly on euchromatin. We confirmed the nuclear localization of FADD in ex vivo liver and showed that in this organ FADD and MBD4 interact together. These results demonstrate that FADD is physiologically expressed in the nucleus of cells in at least two mouse organs. This particular localization opens new possible role of FADD in vivo either asan inhibitor of cell death, or as a transcription factor, or as a molecular link between apoptosis and genome surveillance

    Révision des espèces de Cladochonus McCoy, 1847 (Tabulata) décrites dans le Frasnien de la Belgique par LECOMPTE en 1939 - Cladochonus frasniens de Belgique

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    The original material of the three species from the Frasnian of Belgium formerly assigned to the genus Cladochonus McCoy, 1847 by LECOMPTE (1939) has been revised. Two of these species are considered valid and maintained in the genus: C. robustus LECOMPTE, 1939 and C. maillieuxi LECOMPTE, 1939; C. brevicollis devonicus is for us a synonym of the last species. A microstructural study of C. robustus affords a useful comparison with the microstructure of typical Cladochonus from the Carboniferous. A systematical hierarchy of characters is proposed for the family Pyrgiidae DE FROMENTEL, 1861

    Sedimentologie et coraux du bioherme de marbre rouge Frasnien (F2j) de Tapoumont (Massif de Phillippeville, Belgique)

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    The sedimentological study of the Frasnian Tapoumont red marble bioherm (Philippeville antiform, Belgium, «F2j» based on rugose corals) enables recognition of twelve microfacies. They range from sponge spicule mudstones to crinoidal, coral rudstones. Water agitation and light supply normally control the bioherm growth, but three environments do not follow the rule. They are characterized by the abundance of microorganic mats, now in form of irregular plurimetric fenestrae. cemented by radiaxial calcite. They are more and more abundant towards the centre of the buildup. Four lateral microfacies are studied. The bioherm geometry is discussed, and a weak sedimentary slope is proposed

    Sedimentologie paleoecologie et paleontologie des calcaires crinoidiques au voisinage de la limite couvinien-givetien a Wellin

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    The sedimentology of the Eifelian-Givetian boundary beds in the Wellin area indicates a transition from a siliciclastic-carbonate ramp to a gently sloping carbonate platform lacking a true reefal barrier. Prograding decametric sedimentary units form a littoral accretion megasequence. Corallian megafauna and algal microflora are highly diverse and abundant. Several communities are recognized in the ramp but they cannot be used for precise chronostratigraphic correlations.Comparison with other regions of the Dinant Synclinorium shows that the basin was already partitioned into blocks in Late Eifelian time. In Early Givetian several blocks of plurikilometric extension are identified. In this context, recognition of a new formation, provisionnally called "Formation X", between the Jemelle and Hanonet Formations, is significant.This local unit, some 120 meters thick, is composed of crinoidal and reefal limestones and indicates the existence of a sharply subsiding block in the Wellin area

    Gene synthétique cryic et plantes transgéniques exprimant ledit gène

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    L'invention est relative à un gène synthétique codant pour une toxine CryIC de Bacillus thuringiensis, et à des plantes transgéniques exprimant ce gène

    Cycle de développement et tables de vie de Parlatoria blanchardi Targ. (Homoptera : Diaspididae) et de son prédateur éxotique en Mauritanie, Chilocorus bipustulatus L. var. Iranensis (Coleoptera : Coccinellidae)

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    On a calculé pour Parlatoria blanchardi et son prédateur: Chilocorus bipustulatus, le taux intrinsèque d'accroissement de la population (R), le taux de multiplication par femelle et par jour, le potentiel net de reproduction, la contribution de chaque groupe d'âge à la valeur finale de "R". La comparaison de ces paramètres entre eux pour chaque espèce (à 24-25 degrés C) est très favorable à la coccinelle introduite. Mauritanie. Palmier-dattie

    Estimation du "degré d'infestation" du palmier-dattier par Parlatoria blanchardi TARG. (Hemiptera-Diaspididae)

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    Aprés avoir défini 3 zones différentes dans la frondaison du palmier, les auteurs leur attribuent une note en relation avec leur degré d'infestation par Parlatoria blanchardi (pas de cochenille: 0; infestation maximale:5). Différentes fréquences d'observation ont été etudiées. On peut suivre ainsi l'évolution des populations et connaître avec précision les périodes de multiplication de la cochenille. Il faut tenir compte de la note générale des plants surtout lorsque la parcelle est en voie d'assainissement grâce à Chilocorus bipustulatus var. Iranensis. Mauritani

    Déconvolution aveugle en microscopie de fluorescence 3D

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    National audienceDans ce papier, nous proposons une méthode de déconvolution aveugle estimant la fonction de flou (PSF) et l'image nette en microscopie de fluorescence 3D. L'idée est d'utiliser un modèle paramétré par la fonction pupille pour la PSF en décomposant le module et la phase de la partie aberration sur la base de Zernike. Les résultats tant sur des simulations que sur des données expérimentales montrent la supériorité de notre méthode comparée à l'état de l'art
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