Regulation of stanniocalcin-1 secretion by BeWo cells and first trimester human placental tissue from normal pregnancies and those at increased risk of developing preeclampsia.

Stanniocalcin-1 (STC-1) is a multi-functional glycosylated peptide present in the plasma of healthy women postpartum and increased further in pregnancies complicated by preeclampsia. Although the STC-1 gene is expressed by the placenta what regulates its secretion and from which cells at the feto-maternal interface is unknown. Here, we demonstrate for the first time that the syncytiotrophoblast and cytotrophoblast are a major site of STC-1 protein expression in first trimester placental tissue. Further, in response to low oxygen, first trimester chorionic villous tissue from pregnancies at increased risk of developing preeclampsia secreted significantly more STC-1 than normal tissue under the same conditions. Using the human trophoblast cell line BeWo we have shown that low oxygen increased the secretion of STC-1 but it required co-stimulation with the Adenosine-3', 5'-cyclic monophosphate (cAMP) analogue, 8-Bromo adenosine-3', 5'-cyclic monophosphate cAMP (8 Br-cAMP) to reach significance. Inhibition of Hypoxia inducible factor 2α (HIF-2α) and the Phosphatidylinositol-3 kinase (PI3 -Kinase)/AKT/Serum and glucocorticoid-induced kinase-1(SGK-1) pathway resulted in significant inhibition of STC-1 secretion. As both low oxygen and cAMP are known to play a central role in placental function, their regulation of STC-1 points to a potentially important role in the maintenance of a normal healthy pregnancy and we would hypothesize that it may act to protect against prolonged placental hypoxia seen in preeclampsia

A Socioeconomic Well-Being Index

An annual well-being index constructed from thirteen socioeconomic factors is proposed in order to dynamically measure the mood of the US citizenry. Econometric models are fitted to the log-returns of the index in order to quantify its tail risk and perform option pricing and risk budgeting. By providing a statistically sound assessment of socioeconomic content, the index is consistent with rational finance theory, enabling the construction and valuation of insurance-type financial instruments to serve as contracts written against it. Endogenously, the VXO volatility measure of the stock market appears to be the greatest contributor to tail risk. Exogenously, "stress-testing" the index against the politically important factors of trade imbalance and legal immigration, quantify the systemic risk. For probability levels in the range of 5% to 10%, values of trade below these thresholds are associated with larger downward movements of the index than for immigration at the same level. The main intent of the index is to provide early-warning for negative changes in the mood of citizens, thus alerting policy makers and private agents to potential future market downturns

Genetic complexity of miscanthus cell wall composition and biomass quality for biofuels

BACKGROUND: Miscanthus sinensis is a high yielding perennial grass species with great potential as a bioenergy feedstock. One of the challenges that currently impedes commercial cellulosic biofuel production is the technical difficulty to efficiently convert lignocellulosic biomass into biofuel. The development of feedstocks with better biomass quality will improve conversion efficiency and the sustainability of the value-chain. Progress in the genetic improvement of biomass quality may be substantially expedited by the development of genetic markers associated to quality traits, which can be used in a marker-assisted selection program. RESULTS: To this end, a mapping population was developed by crossing two parents of contrasting cell wall composition. The performance of 182 F1 offspring individuals along with the parents was evaluated in a field trial with a randomized block design with three replicates. Plants were phenotyped for cell wall composition and conversion efficiency characters in the second and third growth season after establishment. A new SNP-based genetic map for M. sinensis was built using a genotyping-by-sequencing (GBS) approach, which resulted in 464 short-sequence uniparental markers that formed 16 linkage groups in the male map and 17 linkage groups in the female map. A total of 86 QTLs for a variety of biomass quality characteristics were identified, 20 of which were detected in both growth seasons. Twenty QTLs were directly associated to different conversion efficiency characters. Marker sequences were aligned to the sorghum reference genome to facilitate cross-species comparisons. Analyses revealed that for some traits previously identified QTLs in sorghum occurred in homologous regions on the same chromosome. CONCLUSION: In this work we report for the first time the genetic mapping of cell wall composition and bioconversion traits in the bioenergy crop miscanthus. These results are a first step towards the development of marker-assisted selection programs in miscanthus to improve biomass quality and facilitate its use as feedstock for biofuel production

Boron Stress Responsive MicroRNAs and Their Targets in Barley

Boron stress is an environmental factor affecting plant development and production. Recently, microRNAs (miRNAs) have been found to be involved in several plant processes such as growth regulation and stress responses. In this study, miRNAs associated with boron stress were identified and characterized in barley. miRNA profiles were also comparatively analyzed between root and leave samples. A total of 31 known and 3 new miRNAs were identified in barley; 25 of them were found to respond to boron treatment. Several miRNAs were expressed in a tissue specific manner; for example, miR156d, miR171a, miR397, and miR444a were only detected in leaves. Additionally, a total of 934 barley transcripts were found to be specifically targeted and degraded by miRNAs. In silico analysis of miRNA target genes demonstrated that many miRNA targets are conserved transcription factors such as Squamosa promoter-binding protein, Auxin response factor (ARF), and the MYB transcription factor family. A majority of these targets were responsible for plant growth and response to environmental changes. We also propose that some of the miRNAs in barley such as miRNA408 might play critical roles against boron exposure. In conclusion, barley may use several pathways and cellular processes targeted by miRNAs to cope with boron stress

On a family of numerical models for couple stress based flexoelectricity for continua and beams

A family of numerical models for the phenomenological linear flexoelectric theory for continua and their particularisation to the case of three-dimensional beams based on a skew-symmetric couple stress theory is presented. In contrast to the standard strain gradient flexoelectric models which assume coupling between electric polarisation and strain gradients, we postulate an electric enthalpy in terms of linear invariants of curvature and electric field. This is achieved by introducing the axial (mean) curvature vector as a strain gradient measure. The physical implication of this assumption is many-fold. Firstly, analogous to the standard strain gradient models, for isotropic (non-piezoelectric) materials it allows constructing flexoelectric energies without breaking material’s centrosymmetry. Secondly, unlike the standard strain gradient models, nonuniform distribution of volumetric part of strains (volumetric strain gradients) do not generate electric polarisation, as also confirmed by experimental evidence to be the case for some important classes of flexoelectric materials. Thirdly, a state of plane strain generates out of plane deformation through strain gradient effects. Finally, under this theory, extension and shear coupling modes cannot be characterised individually as they contribute to the generation of electric polarisation as a whole. As a first step, a detailed comparison of the developed couple stress based flexoelectric model with the standard strain gradient flexoelectric models is performed for the case of Barium Titanate where a myriad of simple analytical solutions are assumed in order to quantitatively describe the similarities and dissimilarities in effective electromechanical coupling under these two theories. From a physical point of view, the most notable insight gained is that, if the same experimental flexoelectric constants are fitted in to both theories, the presented theory in general, reports up to 200% stronger electromechanical conversion efficiency. From the formulation point of a view, the presented flexoelectric model is also competitively simpler as it eliminates the need for high order strain gradient and coupling tensors and can be characterised by a single flexoelectric coefficient. In addition, three distinct mixed flexoelectric variational principles are presented for both continuum and beam models that facilitate incorporation of strain gradient measures in to a standard finite element scheme while maintaining the C0 continuity. Consequently, a series of low and high order mixed finite element schemes for couple stress based flexoelectricity are presented and thoroughly benchmarked against available closed form solutions in regards to electromechanical coupling efficiency. Finally, nanocompression of a complex flexoelectric conical pyramid for which analytical solution cannot be established is numerically studied where curvature induced necking of the specimen and vorticity around the frustum generate moderate electric polarisation

Left ventricular free wall impeding rupture in post-myocardial infarction period diagnosed by myocardial contrast echocardiography: Case report

BACKGROUND: Left ventricular free wall rupture occurs in up to 10% of the in-hospital deaths following myocardial infarction. It is mainly associated with posterolateral myocardial infarction and its antemortem diagnosis is rarely made. Contrast echocardiography has been increasingly used for the evaluation of myocardial perfusion in patients with acute myocardial infarction, with important prognostic implications. In this case, we reported its use for the detection of a mechanical complication following myocardial infarction. CASE PRESENTATION: A 50-year-old man with acute myocardial infarction in the lateral wall underwent myocardial contrast echocardiography for the evaluation of myocardial perfusion in the third day post-infarction. A perfusion defect was detected in lateral and inferior walls as well as the presence of contrast extrusion from the left ventricular cavity into the myocardium, forming a serpiginous duct extending from the endocardium to the epicardial region of the lateral wall, without communication with the pericardial space. Magnetic resonance imaging confirmed the diagnosis of impending rupture of the left ventricular free wall. While waiting for cardiac surgery, patient presented with cardiogenic shock and died. Anatomopathological findings were consistent with acute myocardial infarction in the lateral wall and a left ventricular free wall rupture at the infarct site. CONCLUSION: This case illustrates the early diagnosis of left ventricular free wall rupture by contrast echocardiography. Due to its ability to be performed at bedside this modality of imaging has the potential to identify this catastrophic condition in patients with acute myocardial infarction and help to treat these patients with emergent surgery

Relato de caso: a importância da atuação multiprofissional na laringectomia supracricóide

Esse estudo tem como tema a atuação multiprofissional na laringectomia supracricóide. A terapia fonoaudiológica no hospital foi realizada duas vezes por dia, após 14 sessões, a consistência pastosa foi liberada. O paciente retornou ao ambulatório de disfagia do hospital, uma vez por semana durante dois meses e atualmente retorna uma vez ao mês. A terapia nutricional por sonda nasoentérica (SNE) possibilitou a ingestão calórica necessária e hidratação e, com a liberação da consistência via oral pelo Fonoaudiólogo, o Nutricionista ampliou as opções de alimentos que o paciente poderia ingerir, favorecendo o ganho de peso e retirada da via alternativa de alimentação. A intervenção multiprofissional possibilitou que o grau de disfagia orofaríngea mecânica evoluísse de grave para disfagia orofaríngea leve apenas para líquidos, sendo a evolução da terapia constatada por meio da ingestão oral do paciente, a qual evoluiu da FOIS 1 para FOIS 3 e atualmente encontra-se na FOIS 6. A realização da terapia nutricional enteral possibilitou que o paciente recuperasse o seu peso usual, no período de três meses, haja vista que com a cirurgia, o mesmo perdeu 11 Kg. Verificou-se que por meio do acompanhamento multiprofissional, foi possível evoluir de uma nutrição enteral exclusiva para dieta por via oral, o que favoreceu a evolução do estado nutricional, com a recuperação do peso corpóreo, além de proporcionar melhora na qualidade de vida deste sujeito