Computational characterization and evaluation of deformation behavior of spherulite of high density polyethylene in mesoscale domain

We present a rigorously validated and highly sensitive confirmatory real-time RT-PCR assay (1A assay) that can be used in combination with the previously reported upE assay. Two additional RT-PCR assays for sequencing are described, targeting the RdRp gene (RdRpSeq assay) and N gene (NSeq assay), where an insertion/deletion polymorphism might exist among different hCoV-EMC strains. Finally, a simplified and biologically safe protocol for detection of antibody response by immunofluorescence microscopy was developed using convalescent patient serum

Non-structural genes of novel lemur adenoviruses reveal codivergence of virus and host.

Adenoviruses (AdVs) are important human and animal pathogens and are frequently used as vectors for gene therapy and vaccine delivery. Surprisingly, there are only scant data regarding primate AdV origin and evolution, especially in the most basal primate hosts. We detect and sequence AdVs from faeces of two Madagascan lemur species. Complete genome sequence analyses define a new AdV species with a particularly large gene encoding a protein of unknown function in the early gene region 3. Unexpectedly, the new AdV species is not most similar to human or other simian AdVs but to bat adenovirus C. Genome characterisation shows signals of virus-host codivergence in non-structural genes, which show lower diversity than structural genes. Outside a lemur species mixing zone, recombination less frequently separates structural genes, as in human adenovirus C. The evolutionary history of lemur AdVs likely involves both a host switch and codivergence with the lemur hosts

Non-structural genes of novel lemur adenoviruses reveal codivergence of virus and host

Funder: Landesforschungsförderung HamburgFunder: Berlin Institute of Health (BIH)Adenoviruses (AdVs) are important human and animal pathogens and are frequently used as vectors for gene therapy and vaccine delivery. Surprisingly, there are only scant data regarding primate AdV origin and evolution, especially in the most basal primate hosts. We detect and sequence AdVs from faeces of two Madagascan lemur species. Complete genome sequence analyses define a new AdV species with a particularly large gene encoding a protein of unknown function in the early gene region 3. Unexpectedly, the new AdV species is not most similar to human or other simian AdVs but to bat adenovirus C. Genome characterisation shows signals of virus–host codivergence in non-structural genes, which show lower diversity than structural genes. Outside a lemur species mixing zone, recombination less frequently separates structural genes, as in human adenovirus C. The evolutionary history of lemur AdVs likely involves both a host switch and codivergence with the lemur hosts

Non-structural genes of novel lemur adenoviruses reveal codivergence of virus and host

Abstract Adenoviruses are important human and animal pathogens, and are frequently used as vectors for gene therapy and vaccine delivery. Surprisingly, there are only scant data regarding primate adenovirus origin and evolution, especially in the most basal primate hosts. We detect and sequence adenoviruses from faeces of two Madagascan lemur species. Complete genome sequence analyses define a new adenovirus species with a particularly large gene encoding a protein of unknown function in the early gene region 3. Unexpectedly, the new adenovirus species is not most similar to human or other simian adenoviruses, but to bat adenovirus C. Genome characterisation shows signals of virus-host codivergence in non-structural genes, which show lower diversity than structural genes. Similar to the recombination pattern observed in human adenovirus C, recombination less frequently separates structural genes. However, with the exception of the recombination pattern in a lemur species mixing zone. The evolutionary history of lemur adenoviruses likely involves both a host switch and codivergence with the lemur hosts.</jats:p