Methodological framework for projecting the potential loss of intraspecific genetic diversity due to global climate change

Background: While research on the impact of global climate change (GCC) on ecosystems and species is flourishing, a fundamental component of biodiversity -- molecular variation -- has not yet received its due attention in such studies. Here we present a methodological framework for projecting the loss of intraspecific genetic diversity due to GCC. Methods: The framework consists of multiple steps that and combines 1) hierarchical genetic clustering methods to define comparable units of inference, 2) species accumulation curves (SAC) to infer sampling completeness, and 3) species distribution modelling (SDM) to project the genetic diversity loss under GCC. We suggest procedures for existing data sets as well as specifically designed studies. We illustrate the approach with two worked examples from a land snail (Trochulus villosus) and a caddisfly (Smicridea (S.) mucronata). Results: Sampling completeness was diagnosed on the third most coarse haplotype clade level for T. villosus and the second most coarse for S. mucronata. For both species, a substantial species range loss was projected under the chosen climate scenario. However, despite substantial differences in data set quality concerning spatial sampling and sampling depth, no loss of haplotype clades due to GCC was predicted for either species. Conclusions: The suggested approach presents a feasible method to tap the rich resources of existing phylogeographic data sets and guide the design and analysis of studies explicitly designed to estimate the impact of GCC on a currently still neglected level of biodiversity

Access via the internal jugular vein

Central venous access via the internal jugular vein (IJV) is safe, relatively easy and very commonly used in infants and children undergoing cardiac surgery for congenital heart disease. Because of the wide range of anatomical variations an ultrasound-guided technique is advantageous in many cases, in particular in patients who have had previous punctures or those in whom difficulties are anticipated for various reasons. The right internal jugular vein is the preferred vein for central venous access as it offers straight access to the superior vena cava. The rate of complications - insertion-related as well as long term - are lower compared to the femoral and the subclavian access.peer-reviewe

Access via the femoral vein

Central venous access via the femoral vein (FV) is safe, relatively easy and very usual in infants and children undergoing cardiac surgery for congenital heart disease. It has a low insertion-related complication rate. It is therefore a good choice for short-term central venous lines and a preferred insertion site for less experienced staff. The maintenance-related complications of thrombus formation and infections are higher compared to the internal jugular and the subclavian venous access. Some of these complications are reduced by the use of heparin bonded catheters, routine use of antibiotics, and timely removal of these lines in patients with persistent signs of infection but without another focus being defined.peer-reviewe

Solvent free model for self-assembling fluid bilayer membranes: Stabilization of the fluid phase based on broad attractive tail potentials

We present a simple and highly adaptable method for simulating coarse-grained lipid membranes without explicit solvent. Lipids are represented by one head-bead and two tail-beads, with the interaction between tails being of key importance in stabilizing the fluid phase. Two such tail-tail potentials were tested, with the important feature in both cases being a variable range of attraction. We examined phase diagrams of this range versus temperature for both functional forms of the tail-tail attraction and found that a certain threshold attractive width was required to stabilize the fluid phase. Within the fluid phase region we find that material properties such as area per lipid, orientational order, diffusion constant, inter-leaflet flip-flop rate and bilayer stiffness all depend strongly and monotonically on the attractive width. For three particular values of the potential width we investigate the transition between gel and fluid phases via heating or cooling and find that this transition is discontinuous with considerable hysteresis. We also investigated the stretching of a bilayer to eventually form a pore and found excellent agreement with a recently published analytic theory.Comment: 14 pages 12 figure

Health insurance and consumer welfare : The case of monopolistic drug markets

Individual moral hazard engendered by health insurance and monopolistic production are both typical phenomena of drug markets. We develop a simple model containing these two elements and show that private agents tend to overinsure themselves against health respectively drug expenses if drugs can be produced at low marginal costs. If marginal costs are negligible, health insurance should be abandoned at all

Radiative corrections to the pressure and the one-loop polarization tensor of massless modes in SU(2) Yang-Mills thermodynamics

We compute the one-loop polarization tensor $\Pi$ for the on-shell, massless mode in a thermalized SU(2) Yang-Mills theory being in its deconfining phase. Postulating that SU(2)$_{\tiny{CMB}}\stackrel{\tiny{today}}=U(1)_Y$, we discuss $\Pi$'s effect on the low-momentum part of the black-body spectrum at temperatures $\sim 2... 4$ $T_{\tiny{CMB}}$ where $T_{\tiny{CMB}}\sim 2.73$K. A table-top experiment is proposed to test the above postulate. As an application, we point out a possible connection with the stability of dilute, cold, and old innergalactic atomic hydrogen clouds. We also compute the two-loop correction to the pressure arising from the instantaneous massless mode in unitary-Coulomb gauge, which formerly was neglected, and present improved estimates for subdominant corrections.Comment: 25 pages, 17 figs, v4: consequences of a modification of the evolution equation for the effectice coupling implemented, no qualitative change of the physic

Shape oscillations of human neutrophil leukocytes: characterization and relationship to cell motility

When neutrophil leukocytes are stimulated by chemotactic factors or by substratum contact, they change their shape. Shape changes are a prerequisite for cellular migration and typically involve the extrusion of thin, veil-like lamellipods and the development of morphological polarity. Stimulation also leads to changes in the neutrophil content of filamentous actin (F-actin), which is the major cytoskeletal component. Suspensions of human neutrophils stimulated with chemoattractants exhibit sinusoidal light-scattering oscillations with a period of approximately 8 s at 37°C. These oscillations arise from periodic fluctuations in the cell body size caused by lamellipod extension and retraction cycles. The light-scattering oscillations are paralleled by corresponding oscillations in F-actin content. This raises the interesting possibility that cyclic actin polymerization constitutes the driving force for shape oscillations of suspended neutrophils. Similar periodic shape changes are present in neutrophils crawling on a surface, suggesting that shape oscillations are important for neutrophil motion. This review summarizes our present knowledge about shape oscillations in suspended and crawling neutrophils and discusses a possible role for these oscillations in neutrophil motility