Age at death estimation from bone histology in Malaysian males.

Estimation of age from microscopic examination of human bone utilizes bone remodeling. This allows 2 regression equation to be determined in a specific population based on the variation in osteon turnover in different populations. The aim of this study was to provide age estimation for Malaysian males. Ground undecalcified cross sections were prepared from long limb bones of 50 deceased males aged between 21 and 78 years. Ten microstructural parameters were measured and subjected to multivariate regression analysis. Results showed that osteon count had the highest correlation with age (R = 0.43), and age was estimated to be within 10.94 years of the true value in 98% of males. Cross validation of the equation on 50 individuals showed close correspondence of true ages with estimated ages. Further studies are needed to validate and expand these results

Evaluating osteological ageing from digital data

YesAge at death estimation of human skeletal remains is one of the key issues in constructing a biological profile both in forensic and archaeological contexts. The traditional adult osteological methods evaluate macroscopically the morphological changes that occur with increasing age of specific skeletal indicators, such as the cranial sutures, the pubic bone, the auricular surface of the ilium and the sternal end of the ribs. Technologies such as CT and laser scanning are becoming more widely used in anthropology, and several new methods have been developed. This review focuses on how the osteological age-related changes have been evaluated in digital data. Firstly, the 3D virtual copies of the bones have been used to mimic the appearance of the dry bones and the application of the traditional methods. Secondly, the information directly extrapolated from CT scan has been used to qualitatively or quantitatively assess the changes of the trabecular bones, the thickness of the cortical bones, and to perform morphometric analyses. Lastly, the most innovative approach has been the mathematical quantification of the changes of the pelvic joints, calculating the complexity of the surface. The importance of new updated reference datasets, created thanks to the use of CT scanning in forensic settings, is also discussed.CV was supported from the Danish Council for Independent Research (DFF – 4005-00102B – FTP

Evaluating elbow osteoarthritis within the prehistoric Tiwanaku state using generalized estimating equations (GEE).

OBJECTIVES:Studies of osteoarthritis (OA) in human skeletal remains can come with scalar problems. If OA measurement is noted as present or absent in one joint, like the elbow, results may not identify specific articular pathology data and the sample size may be insufficient to address research questions. If calculated on a per data point basis (i.e., each articular surface within a joint), results may prove too data heavy to comprehensively understand arthritic changes, or one individual with multiple positive scores may skew results and violate the data independence required for statistical tests. The objective of this article is to show that the statistical methodology Generalized Estimating Equations (GEE) can solve scalar issues in bioarchaeological studies. MATERIALS AND METHODS:Using GEE, a population-averaged statistical model, 1,195 adults from the core and one colony of the prehistoric Tiwanaku state (AD 500-1,100) were evaluated bilaterally for OA on the seven articular surfaces of the elbow joint. RESULTS:GEE linked the articular surfaces within each individual specimen, permitting the largest possible unbiased dataset, and showed significant differences between core and colony Tiwanaku peoples in the overall elbow joint, while also pinpointing specific articular surfaces with OA. Data groupings by sex and age at death also demonstrated significant variation. A pattern of elbow rotation noted for core Tiwanaku people may indicate a specific pattern of movement. DISCUSSION:GEE is effective and should be encouraged in bioarchaeological studies as a way to address scalar issues and to retain all pathology information

A Statistical Method for Reassociating Human Tali and Calcanei from a Commingled Context.

In a commingled context, assessing that a talus and a calcaneus correspond to the same individual could become a primary step for accurately sorting human remains. For this purpose, the lengths and widths of the trochlea, posterior calcaneal articular surface, and posterior talar articular surface were measured in 197 individuals (105 males, 92 females) from the Athens Collection. A total of 12 highly accurate equations for reassociating tali and calcanei were developed, using simple and multiple linear regression analysis and they were found to be suitable for sorting commingled human remains. Bilateral asymmetry and sex did not have an effect on the accuracy of the method

Interview with Joe Zvolanek

An interview with Joe Zvolanek about conservation practices. Audio is on tape MS016_13-2_71-030https://scholars.fhsu.edu/koh/1082/thumbnail.jp

Release of Lungworm Larvae from Snails in the Environment: Potential for Alternative Transmission Pathways

Background: Gastropod-borne parasites may cause debilitating clinical conditions in animals and humans following the consumption of infected intermediate or paratenic hosts. However, the ingestion of fresh vegetables contaminated by snail mucus and/or water has also been proposed as a source of the infection for some zoonotic metastrongyloids (e.g., Angiostrongylus cantonensis). In the meantime, the feline lungworms Aelurostrongylus abstrusus and Troglostrongylus brevior are increasingly spreading among cat populations, along with their gastropod intermediate hosts. The aim of this study was to assess the potential of alternative transmission pathways for A. abstrusus and T. brevior L3 via the mucus of infected Helix aspersa snails and the water where gastropods died. In addition, the histological examination of snail specimens provided information on the larval localization and inflammatory reactions in the intermediate host. Methodology/Principal Findings: Twenty-four specimens of H. aspersa received ~500 L1 of A. abstrusus and T. brevior, and were assigned to six study groups. Snails were subjected to different mechanical and chemical stimuli throughout 20 days in order to elicit the production of mucus. At the end of the study, gastropods were submerged in tap water and the sediment was observed for lungworm larvae for three consecutive days. Finally, snails were artificially digested and recovered larvae were counted and morphologically and molecularly identified. The anatomical localization of A. abstrusus and T. brevior larvae within snail tissues was investigated by histology. L3 were detected in the snail mucus (i.e., 37 A. abstrusus and 19 T. brevior) and in the sediment of submerged specimens (172 A. abstrusus and 39 T. brevior). Following the artificial digestion of H. aspersa snails, a mean number of 127.8 A. abstrusus and 60.3 T. brevior larvae were recovered. The number of snail sections positive for A. abstrusus was higher than those for T. brevior. Conclusions: Results of this study indicate that A. abstrusus and T. brevior infective L3 are shed in the mucus of H. aspersa or in water where infected gastropods had died submerged. Both elimination pathways may represent alternative route(s) of environmental contamination and source of the infection for these nematodes under field conditions and may significantly affect the epidemiology of feline lungworms. Considering that snails may act as intermediate hosts for other metastrongyloid species, the environmental contamination by mucus-released larvae is discussed in a broader context

Contributions of pathological alterations to forensic anthropology interpretation

Pathology plays a key role in various aspects of interpretation within forensic anthropology.  These contributions include of the effects of pathological conditions and using them to facilitate identification efforts.  In the context of the broader definition of pathology as “something abnormal” it forms the foundation of much of the logic, methodology and practice of forensic   In fact, only unusual conditions not by many others can be utilized for positive identification.  Recovery efforts and evaluation of the evidence of foul play, as well as the estimation sex, age at death, ancestry, living stature, time since death and other important components depend upon recognition of normal and abnormal patterns.  The multifaceted contributions of pathology to the practice of forensic are firmly documented in casework applications.Pathology plays a key role in various aspects of interpretation within forensic anthropology.  These contributions include observations of the effects of pathological conditions and using them to facilitate identification efforts.  In the context of the broader definition of pathology as “something abnormal” it forms the foundation of much of the logic, methodology and practice of forensic anthropology.  In fact, only unusual conditions not shared by many others can be utilized for positive identification.  Recovery efforts and evaluation of the evidence of foul play, as well as the estimation sex, age at death, ancestry, living stature, time since death and other important components depend upon recognition of normal and abnormal patterns.  The multifaceted contributions of pathology to the practice of forensic anthropology are firmly documented in casework application

Fine Structure of Nucleoli in Cells of Encysted Hymenolepis diminuta

Studies of the fine structure of 5 day old cysticercoids of H. diminuta revealed nucleoli with well-formed lamellae in germinal cells within the body of the encysted worm. The nucleoli were located centrally in the nucleus and appeared not to be attached to the chromosomes, chromatin, or to the nuclear envelope

A Living Skills Curriculum for Adults with Developmental Disabilities

The purpose of this project was to develop a living skills curriculum for adults with developmental disabilities. It will present many of the necessary skills for succeeding at living independently in the community. This curriculum was designed to be taught in a community college setting, using the ten week quarter system. To accomplish this purpose, a review of current literature and research was conducted. The review found few curricula available for teaching living skills and none on the postsecondary level. The research established a need for a curriculum ofliving skills for adults and this project is offered in partial fulfillment of that need